Specific TaqMan probed real-time quantitative RT-PCR methods and their application to differentiate the transcripts of duplicated BF or BLB genes in chicken MHC
BF and BLB genes of chicken major histocompatibility complex (MHC) are responsible for classical antigen processing and presentation; therefore they play a central role in determining the genetic resistance or susceptibility of different MHC haplotypes to some infectious diseases. In this study, we...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2012
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/16368 |
| _version_ | 1855519088292397056 |
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| author | Cai-Xia Gao Lingxia Han Liandong Qu Yu-Zhu Luo Han Jianlin |
| author_browse | Cai-Xia Gao Han Jianlin Liandong Qu Lingxia Han Yu-Zhu Luo |
| author_facet | Cai-Xia Gao Lingxia Han Liandong Qu Yu-Zhu Luo Han Jianlin |
| author_sort | Cai-Xia Gao |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | BF and BLB genes of chicken major histocompatibility complex (MHC) are responsible for classical antigen processing and presentation; therefore they play a central role in determining the genetic resistance or susceptibility of different MHC haplotypes to some infectious diseases. In this study, we developed specific TaqMan probed real-time quantitative reverse transcription PCR (TaqMan qRT-PCR) methods based on the diagnostic nucleotide polymorphisms present in duplicated BF or BLB genes in B2 and B19 haplotypes. The results showed very similar amplification efficiency but no cross-reaction between the duplicated BF or BLB genes of the same haplotype. Spleen mRNA samples of B2 and B19 chickens were used to validate these TaqMan qRT-PCR methods. We observed that BF2 or BLB2 gene was dominantly transcribed in all B2 and B19 chickens. Our findings verified the impact of diversified promoter sequences on the function of duplicated BF or BLB genes. Hence the principles adopted to establish these specific TaqMan qRT-PCR methods in this study can be applied to differentiate the transcripts of duplicated BF or BLB genes of other MHC-B haplotypes in chicken. |
| format | Journal Article |
| id | CGSpace16368 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace163682025-06-13T04:20:11Z Specific TaqMan probed real-time quantitative RT-PCR methods and their application to differentiate the transcripts of duplicated BF or BLB genes in chicken MHC Cai-Xia Gao Lingxia Han Liandong Qu Yu-Zhu Luo Han Jianlin transcription chickens immunology BF and BLB genes of chicken major histocompatibility complex (MHC) are responsible for classical antigen processing and presentation; therefore they play a central role in determining the genetic resistance or susceptibility of different MHC haplotypes to some infectious diseases. In this study, we developed specific TaqMan probed real-time quantitative reverse transcription PCR (TaqMan qRT-PCR) methods based on the diagnostic nucleotide polymorphisms present in duplicated BF or BLB genes in B2 and B19 haplotypes. The results showed very similar amplification efficiency but no cross-reaction between the duplicated BF or BLB genes of the same haplotype. Spleen mRNA samples of B2 and B19 chickens were used to validate these TaqMan qRT-PCR methods. We observed that BF2 or BLB2 gene was dominantly transcribed in all B2 and B19 chickens. Our findings verified the impact of diversified promoter sequences on the function of duplicated BF or BLB genes. Hence the principles adopted to establish these specific TaqMan qRT-PCR methods in this study can be applied to differentiate the transcripts of duplicated BF or BLB genes of other MHC-B haplotypes in chicken. 2012-02 2012-01-18T06:55:20Z 2012-01-18T06:55:20Z Journal Article https://hdl.handle.net/10568/16368 en Limited Access Elsevier Cai-Xia Gao, Ling-Xia Han, Lian-Dong Qu, Yu-Zhu Luo and Jian-Lin Han. 2012. Specific TaqMan probed real-time quantitative RT-PCR methods and their application to differentiate the transcripts of duplicated BF or BLB genes in chicken MHC. Veterinary Immunology and Immunopathology 145(3-4): 590-596 |
| spellingShingle | transcription chickens immunology Cai-Xia Gao Lingxia Han Liandong Qu Yu-Zhu Luo Han Jianlin Specific TaqMan probed real-time quantitative RT-PCR methods and their application to differentiate the transcripts of duplicated BF or BLB genes in chicken MHC |
| title | Specific TaqMan probed real-time quantitative RT-PCR methods and their application to differentiate the transcripts of duplicated BF or BLB genes in chicken MHC |
| title_full | Specific TaqMan probed real-time quantitative RT-PCR methods and their application to differentiate the transcripts of duplicated BF or BLB genes in chicken MHC |
| title_fullStr | Specific TaqMan probed real-time quantitative RT-PCR methods and their application to differentiate the transcripts of duplicated BF or BLB genes in chicken MHC |
| title_full_unstemmed | Specific TaqMan probed real-time quantitative RT-PCR methods and their application to differentiate the transcripts of duplicated BF or BLB genes in chicken MHC |
| title_short | Specific TaqMan probed real-time quantitative RT-PCR methods and their application to differentiate the transcripts of duplicated BF or BLB genes in chicken MHC |
| title_sort | specific taqman probed real time quantitative rt pcr methods and their application to differentiate the transcripts of duplicated bf or blb genes in chicken mhc |
| topic | transcription chickens immunology |
| url | https://hdl.handle.net/10568/16368 |
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