Mapping seedling and adult plant leaf rust resistance genes in the durum wheat cultivar Strongfield and other Triticum turgidum lines
Durum wheat (Triticum turgidum) is threatened by the appearance of new virulent races of leaf rust, caused by Puccinia triticina, in recent years. This study was conducted to determine the leaf rust resistance in a modern Canadian durum cultivar, Strongfield. Six populations derived from crosses of...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
American Phytopathological Society
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/163373 |
| _version_ | 1855532807826178048 |
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| author | Bokore, Firdissa E. Boyle, Kerry Ruan, Yuefeng McCartney, Curt Hiebert, Colin W. Knox, Ron Xiangyu Pei Reimer, Elsa Ammar, Karim Wentao Zhang Fobert, Pierre Cuthbert, Richard D. Berraies, Samia McCallum, Brent D. |
| author_browse | Ammar, Karim Berraies, Samia Bokore, Firdissa E. Boyle, Kerry Cuthbert, Richard D. Fobert, Pierre Hiebert, Colin W. Knox, Ron McCallum, Brent D. McCartney, Curt Reimer, Elsa Ruan, Yuefeng Wentao Zhang Xiangyu Pei |
| author_facet | Bokore, Firdissa E. Boyle, Kerry Ruan, Yuefeng McCartney, Curt Hiebert, Colin W. Knox, Ron Xiangyu Pei Reimer, Elsa Ammar, Karim Wentao Zhang Fobert, Pierre Cuthbert, Richard D. Berraies, Samia McCallum, Brent D. |
| author_sort | Bokore, Firdissa E. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Durum wheat (Triticum turgidum) is threatened by the appearance of new virulent races of leaf rust, caused by Puccinia triticina, in recent years. This study was conducted to determine the leaf rust resistance in a modern Canadian durum cultivar, Strongfield. Six populations derived from crosses of Strongfield with six tetraploid wheat lines, respectively, were tested at the seedling plant stage with different P. triticina races. Two of the populations were evaluated for adult plant leaf rust infection in Canada and Mexico. A stepwise regression joint linkage quantitative trait locus (QTL) mapping and analysis by MapQTL were performed. Strongfield contributed the majority of QTLs detected, contributing seven QTLs detected in field tests and eight QTLs conditioning seedling resistance. A 1B QTL, QLr-Spa-1B.1, from Strongfield had a significant effect in both Canadian and Mexican field tests and corresponded with Lr46/Yr29. The remaining field QTLs were found in only the Canadian or the Mexican environment, not both. The QTL from Strongfield on 3A, QLr-Spa-3A, conferred seedling resistance to all races tested and had a significant effect in the field in Canada. This is the first report of QLr-Spa-3A and Lr46/Yr29 as key components of genetic resistance in Canadian durum wheat. KASP markers were developed to detect QLr-Spa-3A for use in marker-assisted leaf rust resistance breeding. The susceptible parental lines contributed QTLs on 1A, 2B, and 5B that were effective in Mexican field tests and may be good targets to integrate into modern durum varieties to improve resistance to new durum virulent races. |
| format | Journal Article |
| id | CGSpace163373 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | American Phytopathological Society |
| publisherStr | American Phytopathological Society |
| record_format | dspace |
| spelling | CGSpace1633732025-12-08T10:06:44Z Mapping seedling and adult plant leaf rust resistance genes in the durum wheat cultivar Strongfield and other Triticum turgidum lines Bokore, Firdissa E. Boyle, Kerry Ruan, Yuefeng McCartney, Curt Hiebert, Colin W. Knox, Ron Xiangyu Pei Reimer, Elsa Ammar, Karim Wentao Zhang Fobert, Pierre Cuthbert, Richard D. Berraies, Samia McCallum, Brent D. puccinia recondite quantitative trait loci mapping seedlings single nucleotide polymorphisms triticum durum Durum wheat (Triticum turgidum) is threatened by the appearance of new virulent races of leaf rust, caused by Puccinia triticina, in recent years. This study was conducted to determine the leaf rust resistance in a modern Canadian durum cultivar, Strongfield. Six populations derived from crosses of Strongfield with six tetraploid wheat lines, respectively, were tested at the seedling plant stage with different P. triticina races. Two of the populations were evaluated for adult plant leaf rust infection in Canada and Mexico. A stepwise regression joint linkage quantitative trait locus (QTL) mapping and analysis by MapQTL were performed. Strongfield contributed the majority of QTLs detected, contributing seven QTLs detected in field tests and eight QTLs conditioning seedling resistance. A 1B QTL, QLr-Spa-1B.1, from Strongfield had a significant effect in both Canadian and Mexican field tests and corresponded with Lr46/Yr29. The remaining field QTLs were found in only the Canadian or the Mexican environment, not both. The QTL from Strongfield on 3A, QLr-Spa-3A, conferred seedling resistance to all races tested and had a significant effect in the field in Canada. This is the first report of QLr-Spa-3A and Lr46/Yr29 as key components of genetic resistance in Canadian durum wheat. KASP markers were developed to detect QLr-Spa-3A for use in marker-assisted leaf rust resistance breeding. The susceptible parental lines contributed QTLs on 1A, 2B, and 5B that were effective in Mexican field tests and may be good targets to integrate into modern durum varieties to improve resistance to new durum virulent races. 2024-11-01 2024-12-11T17:04:13Z 2024-12-11T17:04:13Z Journal Article https://hdl.handle.net/10568/163373 en Limited Access American Phytopathological Society Bokore, F. E., Boyle, K., Ruan, Y., McCartney, C. A., Hiebert, C. W., Knox, R. E., Pei, X., Reimer, E., Ammar, K., Zhang, W., Fobert, P., Cuthbert, R. D., Berraies, S., & McCallum, B. D. (2024). Mapping seedling and adult plant leaf rust resistance genes in the durum wheat cultivar strongfield and other triticum turgidum lines. Phytopathology, 114(11), 2401–2411. https://doi.org/10.1094/PHYTO-09-23-0348-R |
| spellingShingle | puccinia recondite quantitative trait loci mapping seedlings single nucleotide polymorphisms triticum durum Bokore, Firdissa E. Boyle, Kerry Ruan, Yuefeng McCartney, Curt Hiebert, Colin W. Knox, Ron Xiangyu Pei Reimer, Elsa Ammar, Karim Wentao Zhang Fobert, Pierre Cuthbert, Richard D. Berraies, Samia McCallum, Brent D. Mapping seedling and adult plant leaf rust resistance genes in the durum wheat cultivar Strongfield and other Triticum turgidum lines |
| title | Mapping seedling and adult plant leaf rust resistance genes in the durum wheat cultivar Strongfield and other Triticum turgidum lines |
| title_full | Mapping seedling and adult plant leaf rust resistance genes in the durum wheat cultivar Strongfield and other Triticum turgidum lines |
| title_fullStr | Mapping seedling and adult plant leaf rust resistance genes in the durum wheat cultivar Strongfield and other Triticum turgidum lines |
| title_full_unstemmed | Mapping seedling and adult plant leaf rust resistance genes in the durum wheat cultivar Strongfield and other Triticum turgidum lines |
| title_short | Mapping seedling and adult plant leaf rust resistance genes in the durum wheat cultivar Strongfield and other Triticum turgidum lines |
| title_sort | mapping seedling and adult plant leaf rust resistance genes in the durum wheat cultivar strongfield and other triticum turgidum lines |
| topic | puccinia recondite quantitative trait loci mapping seedlings single nucleotide polymorphisms triticum durum |
| url | https://hdl.handle.net/10568/163373 |
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