High-throughput phenotyping using hyperspectral indicators supports the genetic dissection of yield in durum wheat grown under heat and drought stress
High-throughput phenotyping (HTP) provides new opportunities for efficiently dissecting the genetic basis of drought-adaptive traits, which is essential in current wheat breeding programs. The combined use of HTP and genome-wide association (GWAS) approaches has been useful in the assessment of comp...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2024
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/163235 |
| _version_ | 1855541086382981120 |
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| author | Mérida-García, Rosa Gálvez, Sergio Solís, Ignacio Martínez-Moreno, Fernando Camino, Carlos Soriano, Jose Miguel Sansaloni, Carolina P. Ammar, Karim Bentley, Alison R. Gonzalez-Dugo, Victoria Zarco-Tejada, Pablo J. Hernandez, Pilar |
| author_browse | Ammar, Karim Bentley, Alison R. Camino, Carlos Gonzalez-Dugo, Victoria Gálvez, Sergio Hernandez, Pilar Martínez-Moreno, Fernando Mérida-García, Rosa Sansaloni, Carolina P. Solís, Ignacio Soriano, Jose Miguel Zarco-Tejada, Pablo J. |
| author_facet | Mérida-García, Rosa Gálvez, Sergio Solís, Ignacio Martínez-Moreno, Fernando Camino, Carlos Soriano, Jose Miguel Sansaloni, Carolina P. Ammar, Karim Bentley, Alison R. Gonzalez-Dugo, Victoria Zarco-Tejada, Pablo J. Hernandez, Pilar |
| author_sort | Mérida-García, Rosa |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | High-throughput phenotyping (HTP) provides new opportunities for efficiently dissecting the genetic basis of drought-adaptive traits, which is essential in current wheat breeding programs. The combined use of HTP and genome-wide association (GWAS) approaches has been useful in the assessment of complex traits such as yield, under field stress conditions including heat and drought. The aim of this study was to identify molecular markers associated with yield (YLD) in elite durum wheat that could be explained using hyperspectral indices (HSIs) under drought field conditions in Mediterranean environments in Southern Spain. The HSIs were obtained from hyperspectral imagery collected during the pre-anthesis and anthesis crop stages using an airborne platform. A panel of 536 durum wheat lines were genotyped by sequencing (GBS, DArTseq) to determine population structure, revealing a lack of genetic structure in the breeding germplasm. The material was phenotyped for YLD and 19 HSIs for six growing seasons under drought field conditions at two locations in Andalusia, in southern Spain. GWAS analysis identified 740 significant marker-trait associations (MTAs) across all the durum wheat chromosomes, several of which were common for YLD and the HSIs, and can potentially be integrated into breeding programs. Candidate gene (CG) analysis uncovered genes related to important plant processes such as photosynthesis, regulatory biological processes, and plant abiotic stress tolerance. These results are novel in that they combine high-resolution hyperspectral imaging at the field scale with GWAS analysis in wheat. They also support the use of HSIs as useful tools for identifying chromosomal regions related to the heat and drought stress response in wheat, and pave the way for the integration of field HTP in wheat breeding programs. |
| format | Journal Article |
| id | CGSpace163235 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1632352025-12-08T10:29:22Z High-throughput phenotyping using hyperspectral indicators supports the genetic dissection of yield in durum wheat grown under heat and drought stress Mérida-García, Rosa Gálvez, Sergio Solís, Ignacio Martínez-Moreno, Fernando Camino, Carlos Soriano, Jose Miguel Sansaloni, Carolina P. Ammar, Karim Bentley, Alison R. Gonzalez-Dugo, Victoria Zarco-Tejada, Pablo J. Hernandez, Pilar hard wheat heat drought stress high-throughput phenotyping yields genome-wide association studies High-throughput phenotyping (HTP) provides new opportunities for efficiently dissecting the genetic basis of drought-adaptive traits, which is essential in current wheat breeding programs. The combined use of HTP and genome-wide association (GWAS) approaches has been useful in the assessment of complex traits such as yield, under field stress conditions including heat and drought. The aim of this study was to identify molecular markers associated with yield (YLD) in elite durum wheat that could be explained using hyperspectral indices (HSIs) under drought field conditions in Mediterranean environments in Southern Spain. The HSIs were obtained from hyperspectral imagery collected during the pre-anthesis and anthesis crop stages using an airborne platform. A panel of 536 durum wheat lines were genotyped by sequencing (GBS, DArTseq) to determine population structure, revealing a lack of genetic structure in the breeding germplasm. The material was phenotyped for YLD and 19 HSIs for six growing seasons under drought field conditions at two locations in Andalusia, in southern Spain. GWAS analysis identified 740 significant marker-trait associations (MTAs) across all the durum wheat chromosomes, several of which were common for YLD and the HSIs, and can potentially be integrated into breeding programs. Candidate gene (CG) analysis uncovered genes related to important plant processes such as photosynthesis, regulatory biological processes, and plant abiotic stress tolerance. These results are novel in that they combine high-resolution hyperspectral imaging at the field scale with GWAS analysis in wheat. They also support the use of HSIs as useful tools for identifying chromosomal regions related to the heat and drought stress response in wheat, and pave the way for the integration of field HTP in wheat breeding programs. 2024-11-22 2024-12-09T18:57:49Z 2024-12-09T18:57:49Z Journal Article https://hdl.handle.net/10568/163235 en Open Access application/pdf Frontiers Media Mérida-García, R., Gálvez, S., Solís, I., Martínez-Moreno, F., Camino, C., Soriano, J. M., Sansaloni, C., Ammar, K., Bentley, A. R., Gonzalez-Dugo, V., Zarco-Tejada, P. J., & Hernandez, P. (2024). High-throughput phenotyping using hyperspectral indicators supports the genetic dissection of yield in durum wheat grown under heat and drought stress. Frontiers in Plant Science, 15, 1470520. https://doi.org/10.3389/fpls.2024.1470520 |
| spellingShingle | hard wheat heat drought stress high-throughput phenotyping yields genome-wide association studies Mérida-García, Rosa Gálvez, Sergio Solís, Ignacio Martínez-Moreno, Fernando Camino, Carlos Soriano, Jose Miguel Sansaloni, Carolina P. Ammar, Karim Bentley, Alison R. Gonzalez-Dugo, Victoria Zarco-Tejada, Pablo J. Hernandez, Pilar High-throughput phenotyping using hyperspectral indicators supports the genetic dissection of yield in durum wheat grown under heat and drought stress |
| title | High-throughput phenotyping using hyperspectral indicators supports the genetic dissection of yield in durum wheat grown under heat and drought stress |
| title_full | High-throughput phenotyping using hyperspectral indicators supports the genetic dissection of yield in durum wheat grown under heat and drought stress |
| title_fullStr | High-throughput phenotyping using hyperspectral indicators supports the genetic dissection of yield in durum wheat grown under heat and drought stress |
| title_full_unstemmed | High-throughput phenotyping using hyperspectral indicators supports the genetic dissection of yield in durum wheat grown under heat and drought stress |
| title_short | High-throughput phenotyping using hyperspectral indicators supports the genetic dissection of yield in durum wheat grown under heat and drought stress |
| title_sort | high throughput phenotyping using hyperspectral indicators supports the genetic dissection of yield in durum wheat grown under heat and drought stress |
| topic | hard wheat heat drought stress high-throughput phenotyping yields genome-wide association studies |
| url | https://hdl.handle.net/10568/163235 |
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