Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas
Cassava frogskin disease (CFSD) emerged in the Americas in the 1970s, but its causal agent has to date remained a mystery. The clonal propagation of cassava, high incidence of mixed infections, unknown alternative hosts, and root symptoms taking two or more crop cycles to develop, have made it diffi...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Nature Portfolio
2024
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| Acceso en línea: | https://hdl.handle.net/10568/162969 |
| _version_ | 1855522204708503552 |
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| author | Jimenez, Jenyfer Caicedo, Sara Pardo, Juan M. Gil-Ordóñez, Alejandra Alvarez-Quinto, Robert Mollov, Dimitre Cuellar, Wilmer J. |
| author_browse | Alvarez-Quinto, Robert Caicedo, Sara Cuellar, Wilmer J. Gil-Ordóñez, Alejandra Jimenez, Jenyfer Mollov, Dimitre Pardo, Juan M. |
| author_facet | Jimenez, Jenyfer Caicedo, Sara Pardo, Juan M. Gil-Ordóñez, Alejandra Alvarez-Quinto, Robert Mollov, Dimitre Cuellar, Wilmer J. |
| author_sort | Jimenez, Jenyfer |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava frogskin disease (CFSD) emerged in the Americas in the 1970s, but its causal agent has to date remained a mystery. The clonal propagation of cassava, high incidence of mixed infections, unknown alternative hosts, and root symptoms taking two or more crop cycles to develop, have made it difficult to identify the causal agent. Consequently, most studies on CFSD have produced a catalogue of pathogens occurring in affected plants. Using a sentinel approach, we captured single-pathogen infections in fields with high incidence of root symptoms. Eight months after being exposed to CFSD, we detected < 6.9% incidence of root symptoms in sentinel plants. Plants were then propagated and transferred to a screenhouse for a second infection cycle and storage root development. Interestingly, molecular diagnostics did not identify an association with phytoplasma or reovirids—pathogens historically reported in CFSD-infected plants—but indicated that single-infections by torradoviruses were sufficient to cause the disease. Further analysis by high-throughput sequencing confirmed the presence of torradoviruses in symptomatic roots and allowed unveiling the occurrence of a second torradovirus species in farmers' fields in Colombia. These new findings should support early interception of infected planting material, development of cassava seed certification standards, breeding and screening for resistance programs, and ultimately significantly reduce the impact of CFSD in cassava. |
| format | Journal Article |
| id | CGSpace162969 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Nature Portfolio |
| publisherStr | Nature Portfolio |
| record_format | dspace |
| spelling | CGSpace1629692025-12-08T10:06:44Z Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas Jimenez, Jenyfer Caicedo, Sara Pardo, Juan M. Gil-Ordóñez, Alejandra Alvarez-Quinto, Robert Mollov, Dimitre Cuellar, Wilmer J. cassava disease control infective agents-infectious agents Cassava frogskin disease (CFSD) emerged in the Americas in the 1970s, but its causal agent has to date remained a mystery. The clonal propagation of cassava, high incidence of mixed infections, unknown alternative hosts, and root symptoms taking two or more crop cycles to develop, have made it difficult to identify the causal agent. Consequently, most studies on CFSD have produced a catalogue of pathogens occurring in affected plants. Using a sentinel approach, we captured single-pathogen infections in fields with high incidence of root symptoms. Eight months after being exposed to CFSD, we detected < 6.9% incidence of root symptoms in sentinel plants. Plants were then propagated and transferred to a screenhouse for a second infection cycle and storage root development. Interestingly, molecular diagnostics did not identify an association with phytoplasma or reovirids—pathogens historically reported in CFSD-infected plants—but indicated that single-infections by torradoviruses were sufficient to cause the disease. Further analysis by high-throughput sequencing confirmed the presence of torradoviruses in symptomatic roots and allowed unveiling the occurrence of a second torradovirus species in farmers' fields in Colombia. These new findings should support early interception of infected planting material, development of cassava seed certification standards, breeding and screening for resistance programs, and ultimately significantly reduce the impact of CFSD in cassava. 2024-11-28 2024-12-03T11:17:27Z 2024-12-03T11:17:27Z Journal Article https://hdl.handle.net/10568/162969 en Open Access application/pdf Nature Portfolio Jimenez, J.; Caicedo, S.; Pardo, J.M.; Gil-Ordóñez, A.; Alvarez-Quinto, R.; Mollov, D.; Cuellar, W.J. (2024) Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas. Scientific Reports 14(1): 29648. ISSN: 2045-2322 |
| spellingShingle | cassava disease control infective agents-infectious agents Jimenez, Jenyfer Caicedo, Sara Pardo, Juan M. Gil-Ordóñez, Alejandra Alvarez-Quinto, Robert Mollov, Dimitre Cuellar, Wilmer J. Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas |
| title | Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas |
| title_full | Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas |
| title_fullStr | Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas |
| title_full_unstemmed | Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas |
| title_short | Single torradovirus infections explain the mysterious cassava frogskin disease in the Americas |
| title_sort | single torradovirus infections explain the mysterious cassava frogskin disease in the americas |
| topic | cassava disease control infective agents-infectious agents |
| url | https://hdl.handle.net/10568/162969 |
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