Carbon usage in yellow-fleshed Manihot esculenta storage roots shifts from starch biosynthesis to cell wall and raffinose biosynthesis via the myo-inositol pathway
Cassava is a crucial staple crop for smallholder farmers in tropical Asia and Sub‐Saharan Africa. Although high yield remains the top priority for farmers, the significance of nutritional values has increased in cassava breeding programs. A notable negative correlation between provitamin A and starc...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/162933 |
| _version_ | 1855542872659460096 |
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| author | Gutschker, S. Ruescher, D. Rabbi, I.Y. Rosado-Souza, L. Pommerrenig, B. Pauly, M. Robertz, S. van Doorn, A.M. Schloreth, A. Neuhaus, H.E. Fernie, A.R. Reinert, S. Sonnewald, U. Zierer, W. |
| author_browse | Fernie, A.R. Gutschker, S. Neuhaus, H.E. Pauly, M. Pommerrenig, B. Rabbi, I.Y. Reinert, S. Robertz, S. Rosado-Souza, L. Ruescher, D. Schloreth, A. Sonnewald, U. Zierer, W. van Doorn, A.M. |
| author_facet | Gutschker, S. Ruescher, D. Rabbi, I.Y. Rosado-Souza, L. Pommerrenig, B. Pauly, M. Robertz, S. van Doorn, A.M. Schloreth, A. Neuhaus, H.E. Fernie, A.R. Reinert, S. Sonnewald, U. Zierer, W. |
| author_sort | Gutschker, S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava is a crucial staple crop for smallholder farmers in tropical Asia and Sub‐Saharan Africa. Although high yield remains the top priority for farmers, the significance of nutritional values has increased in cassava breeding programs. A notable negative correlation between provitamin A and starch accumulation poses a significant challenge for breeding efforts. The negative correlation between starch and carotenoid levels in conventional and genetically modified cassava plants implies the absence of a direct genomic connection between the two traits. The competition among various carbon pathways seems to account for this relationship. In this study, we conducted a thorough analysis of 49 African cassava genotypes with varying levels of starch and provitamin A. Our goal was to identify factors contributing to differential starch accumulation. Considering carotenoid levels as a confounding factor in starch production, we found that yellow‐ and white‐fleshed storage roots did not differ significantly in most measured components of starch or de novo fatty acid biosynthesis. However, genes and metabolites associated with myo‐inositol synthesis and cell wall polymer production were substantially enriched in high provitamin A genotypes. These results indicate that yellow‐fleshed cultivars, in comparison to their white‐fleshed counterparts, direct more carbon toward the synthesis of raffinose and cell wall components. This finding is underlined by a significant rise in cell wall components measured within the 20 most contrasting genotypes for carotenoid levels. Our findings enhance the comprehension of the biosynthesis of starch and carotenoids in the storage roots of cassava. |
| format | Journal Article |
| id | CGSpace162933 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| record_format | dspace |
| spelling | CGSpace1629332025-12-08T09:54:28Z Carbon usage in yellow-fleshed Manihot esculenta storage roots shifts from starch biosynthesis to cell wall and raffinose biosynthesis via the myo-inositol pathway Gutschker, S. Ruescher, D. Rabbi, I.Y. Rosado-Souza, L. Pommerrenig, B. Pauly, M. Robertz, S. van Doorn, A.M. Schloreth, A. Neuhaus, H.E. Fernie, A.R. Reinert, S. Sonnewald, U. Zierer, W. manihot esculenta cassava starch crops carotenoids myoinositol cell walls raffinose provitamins Cassava is a crucial staple crop for smallholder farmers in tropical Asia and Sub‐Saharan Africa. Although high yield remains the top priority for farmers, the significance of nutritional values has increased in cassava breeding programs. A notable negative correlation between provitamin A and starch accumulation poses a significant challenge for breeding efforts. The negative correlation between starch and carotenoid levels in conventional and genetically modified cassava plants implies the absence of a direct genomic connection between the two traits. The competition among various carbon pathways seems to account for this relationship. In this study, we conducted a thorough analysis of 49 African cassava genotypes with varying levels of starch and provitamin A. Our goal was to identify factors contributing to differential starch accumulation. Considering carotenoid levels as a confounding factor in starch production, we found that yellow‐ and white‐fleshed storage roots did not differ significantly in most measured components of starch or de novo fatty acid biosynthesis. However, genes and metabolites associated with myo‐inositol synthesis and cell wall polymer production were substantially enriched in high provitamin A genotypes. These results indicate that yellow‐fleshed cultivars, in comparison to their white‐fleshed counterparts, direct more carbon toward the synthesis of raffinose and cell wall components. This finding is underlined by a significant rise in cell wall components measured within the 20 most contrasting genotypes for carotenoid levels. Our findings enhance the comprehension of the biosynthesis of starch and carotenoids in the storage roots of cassava. 2024-08 2024-12-02T15:39:59Z 2024-12-02T15:39:59Z Journal Article https://hdl.handle.net/10568/162933 en Open Access application/pdf Gutschker, S., Ruescher, D., Rabbi, I.Y., Rosado‐Souza, L., Pommerrenig, B., Pauly, M., ... & Zierer, W. (2024). Carbon usage in yellow‐fleshed Manihot esculenta storage roots shifts from starch biosynthesis to cell wall and raffinose biosynthesis via the myo‐inositol pathway. The Plant Journal, 119(4), 2045-2062. |
| spellingShingle | manihot esculenta cassava starch crops carotenoids myoinositol cell walls raffinose provitamins Gutschker, S. Ruescher, D. Rabbi, I.Y. Rosado-Souza, L. Pommerrenig, B. Pauly, M. Robertz, S. van Doorn, A.M. Schloreth, A. Neuhaus, H.E. Fernie, A.R. Reinert, S. Sonnewald, U. Zierer, W. Carbon usage in yellow-fleshed Manihot esculenta storage roots shifts from starch biosynthesis to cell wall and raffinose biosynthesis via the myo-inositol pathway |
| title | Carbon usage in yellow-fleshed Manihot esculenta storage roots shifts from starch biosynthesis to cell wall and raffinose biosynthesis via the myo-inositol pathway |
| title_full | Carbon usage in yellow-fleshed Manihot esculenta storage roots shifts from starch biosynthesis to cell wall and raffinose biosynthesis via the myo-inositol pathway |
| title_fullStr | Carbon usage in yellow-fleshed Manihot esculenta storage roots shifts from starch biosynthesis to cell wall and raffinose biosynthesis via the myo-inositol pathway |
| title_full_unstemmed | Carbon usage in yellow-fleshed Manihot esculenta storage roots shifts from starch biosynthesis to cell wall and raffinose biosynthesis via the myo-inositol pathway |
| title_short | Carbon usage in yellow-fleshed Manihot esculenta storage roots shifts from starch biosynthesis to cell wall and raffinose biosynthesis via the myo-inositol pathway |
| title_sort | carbon usage in yellow fleshed manihot esculenta storage roots shifts from starch biosynthesis to cell wall and raffinose biosynthesis via the myo inositol pathway |
| topic | manihot esculenta cassava starch crops carotenoids myoinositol cell walls raffinose provitamins |
| url | https://hdl.handle.net/10568/162933 |
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