Physico-chemical characterization of Anopheles gambiae s.l. breeding sites and kdr mutations in urban areas of Cotonou and Natitingou, Benin
Background This study aimed to investigate the relationship between the physicochemical characteristics of An. gambiae s.s. and An. coluzzii breeding sites, the susceptibility profiles to commonly used insecticides in public health, and the underlying insecticide resistance mechanisms. Methods Anop...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/162697 |
| _version_ | 1855528730285309952 |
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| author | Djegbe, I. Hessou-Djossou, D. Boukari, M.Y.G. Nonfodji, O. Tchigossou, G. Djouaka, R. Cornelie, S. Akogbeto, M. Djogbenou, S.L. Chandre, F. |
| author_browse | Akogbeto, M. Boukari, M.Y.G. Chandre, F. Cornelie, S. Djegbe, I. Djogbenou, S.L. Djouaka, R. Hessou-Djossou, D. Nonfodji, O. Tchigossou, G. |
| author_facet | Djegbe, I. Hessou-Djossou, D. Boukari, M.Y.G. Nonfodji, O. Tchigossou, G. Djouaka, R. Cornelie, S. Akogbeto, M. Djogbenou, S.L. Chandre, F. |
| author_sort | Djegbe, I. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Background
This study aimed to investigate the relationship between the physicochemical characteristics of An. gambiae s.s. and An. coluzzii breeding sites, the susceptibility profiles to commonly used insecticides in public health, and the underlying insecticide resistance mechanisms.
Methods
Anopheles breeding sites surveys were conducted in Cotonou and Natitingou in September 2020, January and August 2021. Physicochemical properties and bacterial loads were determined in individual breeding sites. The WHO susceptibility assays were carried out using the female of the emerging adult mosquitoes. Anopheles species were identified through PCR techniques. Kdr L1014F/S, N1575Y and G119S mutations were investigated using TaqMan genotyping assays.
Results
Molecular analysis showed that all mosquitoes analyzed in Cotonou were Anopheles coluzzii, while those of Natitingou were Anopheles gambiae s.s. Fecal coliforms were identified as playing a role in this distribution through their significant influence on the presence of An. coluzzii larvae. WHO susceptibility assay indicated a high level of resistance to deltamethrin in the two cities. The resistance levels to deltamethrin were higher in Cotonou (X2 = 31.689; DF = 1; P < 0.0001). There was a suspected resistance to bendiocarb in Cotonou, whereas the mosquito population in Natitingou was resistant. The kdr L1014F mutation was highly observed in both mosquito populations (frequence: 86–91%), while the Ace-1 mutation was found in a small proportion of mosquitoes. In Cotonou, salinity was the only recorded physicochemical parameter that significantly correlated with the resistance of Anopheles mosquitoes to deltamethrin (P < 0.05). In Natitingou, significant correlations were observed between the allelic frequencies of the kdr L1014F mutation and pH, conductivity, and TDS.
Conclusion
These results indicate a high level of pyrethroid resistance in the anopheles populations of both Cotonou and Natitingou. Moreover, this study report the involvement of abiotic factors influencing Anopheles susceptibility profile. |
| format | Journal Article |
| id | CGSpace162697 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| record_format | dspace |
| spelling | CGSpace1626972025-12-08T10:11:39Z Physico-chemical characterization of Anopheles gambiae s.l. breeding sites and kdr mutations in urban areas of Cotonou and Natitingou, Benin Djegbe, I. Hessou-Djossou, D. Boukari, M.Y.G. Nonfodji, O. Tchigossou, G. Djouaka, R. Cornelie, S. Akogbeto, M. Djogbenou, S.L. Chandre, F. malaria anopheles gambiae breeding chemicophysical properties Background This study aimed to investigate the relationship between the physicochemical characteristics of An. gambiae s.s. and An. coluzzii breeding sites, the susceptibility profiles to commonly used insecticides in public health, and the underlying insecticide resistance mechanisms. Methods Anopheles breeding sites surveys were conducted in Cotonou and Natitingou in September 2020, January and August 2021. Physicochemical properties and bacterial loads were determined in individual breeding sites. The WHO susceptibility assays were carried out using the female of the emerging adult mosquitoes. Anopheles species were identified through PCR techniques. Kdr L1014F/S, N1575Y and G119S mutations were investigated using TaqMan genotyping assays. Results Molecular analysis showed that all mosquitoes analyzed in Cotonou were Anopheles coluzzii, while those of Natitingou were Anopheles gambiae s.s. Fecal coliforms were identified as playing a role in this distribution through their significant influence on the presence of An. coluzzii larvae. WHO susceptibility assay indicated a high level of resistance to deltamethrin in the two cities. The resistance levels to deltamethrin were higher in Cotonou (X2 = 31.689; DF = 1; P < 0.0001). There was a suspected resistance to bendiocarb in Cotonou, whereas the mosquito population in Natitingou was resistant. The kdr L1014F mutation was highly observed in both mosquito populations (frequence: 86–91%), while the Ace-1 mutation was found in a small proportion of mosquitoes. In Cotonou, salinity was the only recorded physicochemical parameter that significantly correlated with the resistance of Anopheles mosquitoes to deltamethrin (P < 0.05). In Natitingou, significant correlations were observed between the allelic frequencies of the kdr L1014F mutation and pH, conductivity, and TDS. Conclusion These results indicate a high level of pyrethroid resistance in the anopheles populations of both Cotonou and Natitingou. Moreover, this study report the involvement of abiotic factors influencing Anopheles susceptibility profile. 2024 2024-11-25T10:44:29Z 2024-11-25T10:44:29Z Journal Article https://hdl.handle.net/10568/162697 en Open Access application/pdf Djègbè, I., Hessou-Djossou, D., Gounou Boukari, M.K.Y., Nonfodji, O., Tchigossou, G., Djouaka, R., ... & Chandre, F. (2024). Physico-chemical characterization of Anopheles gambiae sl breeding sites and kdr mutations in urban areas of Cotonou and Natitingou, Benin. BMC Infectious Diseases, 24(1): 545, 1-12. |
| spellingShingle | malaria anopheles gambiae breeding chemicophysical properties Djegbe, I. Hessou-Djossou, D. Boukari, M.Y.G. Nonfodji, O. Tchigossou, G. Djouaka, R. Cornelie, S. Akogbeto, M. Djogbenou, S.L. Chandre, F. Physico-chemical characterization of Anopheles gambiae s.l. breeding sites and kdr mutations in urban areas of Cotonou and Natitingou, Benin |
| title | Physico-chemical characterization of Anopheles gambiae s.l. breeding sites and kdr mutations in urban areas of Cotonou and Natitingou, Benin |
| title_full | Physico-chemical characterization of Anopheles gambiae s.l. breeding sites and kdr mutations in urban areas of Cotonou and Natitingou, Benin |
| title_fullStr | Physico-chemical characterization of Anopheles gambiae s.l. breeding sites and kdr mutations in urban areas of Cotonou and Natitingou, Benin |
| title_full_unstemmed | Physico-chemical characterization of Anopheles gambiae s.l. breeding sites and kdr mutations in urban areas of Cotonou and Natitingou, Benin |
| title_short | Physico-chemical characterization of Anopheles gambiae s.l. breeding sites and kdr mutations in urban areas of Cotonou and Natitingou, Benin |
| title_sort | physico chemical characterization of anopheles gambiae s l breeding sites and kdr mutations in urban areas of cotonou and natitingou benin |
| topic | malaria anopheles gambiae breeding chemicophysical properties |
| url | https://hdl.handle.net/10568/162697 |
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