Overexpression of TdNACB improves the drought resistance of rice

Drought stress greatly affects disrupts the productivity, ecological structure, physiological and biochemical activities of wheat at different growth stages. However, drought stress tolerance is a complex quantitative trait and involves multiple metabolic pathways. We found that a wild emmer introgr...

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Autores principales: Fangyi Gong, Tian Zhang, Yusen Lu, Velu, Govindan, Ruiqin Liu, Jia Liu, Xiangquan Wang, Dengcai Liu, Youliang Zheng, Lin Huang, Bihua Wu
Formato: Journal Article
Lenguaje:Inglés
Publicado: Elsevier 2024
Materias:
Acceso en línea:https://hdl.handle.net/10568/162655
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author Fangyi Gong
Tian Zhang
Yusen Lu
Velu, Govindan
Ruiqin Liu
Jia Liu
Xiangquan Wang
Dengcai Liu
Youliang Zheng
Lin Huang
Bihua Wu
author_browse Bihua Wu
Dengcai Liu
Fangyi Gong
Jia Liu
Lin Huang
Ruiqin Liu
Tian Zhang
Velu, Govindan
Xiangquan Wang
Youliang Zheng
Yusen Lu
author_facet Fangyi Gong
Tian Zhang
Yusen Lu
Velu, Govindan
Ruiqin Liu
Jia Liu
Xiangquan Wang
Dengcai Liu
Youliang Zheng
Lin Huang
Bihua Wu
author_sort Fangyi Gong
collection Repository of Agricultural Research Outputs (CGSpace)
description Drought stress greatly affects disrupts the productivity, ecological structure, physiological and biochemical activities of wheat at different growth stages. However, drought stress tolerance is a complex quantitative trait and involves multiple metabolic pathways. We found that a wild emmer introgression line BAd7-209 had stronger drought resistance compared with drought resistant wheat Zhongmai 175. The transcriptome analysis found 14,284, 22,383 and 21,451 genes had expression corresponding responsed to drought stress at 24h, 48h, 120h, respectively and significantly enriched in ‘Arginine and proline metabolism’ and ‘Peroxisome’ in BAd7-209. 1666 transcription factors (TFs) related responsed to drought stress in which TdNACB showed high expression at 24h, 48h and 120h and had the closest relationship with TaNAC48 and OsNAC6 in phylogenetic analysis. Overexpression of TdNACB significantly enhanced drought resistance in rice and overexpression lines had significantly higher CAT, POD and SOD activity, Pro content and lower MDA content than those of the WT under drought stress. The result demonstrated that TdNACB positively regulates drought resistance through increasing proline content and enhancing activity of enzyme related to ROS scavenging. The results of this study provides candidate genes for improving wheat drought resistance and guide as reference for studying the molecular mechanisms of wheat drought resistance.
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spelling CGSpace1626552025-10-26T12:56:04Z Overexpression of TdNACB improves the drought resistance of rice Fangyi Gong Tian Zhang Yusen Lu Velu, Govindan Ruiqin Liu Jia Liu Xiangquan Wang Dengcai Liu Youliang Zheng Lin Huang Bihua Wu drought drought resistance rice wheat yield losses Drought stress greatly affects disrupts the productivity, ecological structure, physiological and biochemical activities of wheat at different growth stages. However, drought stress tolerance is a complex quantitative trait and involves multiple metabolic pathways. We found that a wild emmer introgression line BAd7-209 had stronger drought resistance compared with drought resistant wheat Zhongmai 175. The transcriptome analysis found 14,284, 22,383 and 21,451 genes had expression corresponding responsed to drought stress at 24h, 48h, 120h, respectively and significantly enriched in ‘Arginine and proline metabolism’ and ‘Peroxisome’ in BAd7-209. 1666 transcription factors (TFs) related responsed to drought stress in which TdNACB showed high expression at 24h, 48h and 120h and had the closest relationship with TaNAC48 and OsNAC6 in phylogenetic analysis. Overexpression of TdNACB significantly enhanced drought resistance in rice and overexpression lines had significantly higher CAT, POD and SOD activity, Pro content and lower MDA content than those of the WT under drought stress. The result demonstrated that TdNACB positively regulates drought resistance through increasing proline content and enhancing activity of enzyme related to ROS scavenging. The results of this study provides candidate genes for improving wheat drought resistance and guide as reference for studying the molecular mechanisms of wheat drought resistance. 2024-11 2024-11-22T18:22:23Z 2024-11-22T18:22:23Z Journal Article https://hdl.handle.net/10568/162655 en Limited Access Elsevier Gong, F., Zhang, T., Lu, Y., Velu, G., Liu, R., Liu, J., Wang, X., Liu, D., Zheng, Y., Huang, L., & Wu, B. (2024). Overexpression of TdNACB Improves the Drought Resistance of Rice. Plant Physiology And Biochemistry, 216, 109157. https://doi.org/10.1016/j.plaphy.2024.109157
spellingShingle drought
drought resistance
rice
wheat
yield losses
Fangyi Gong
Tian Zhang
Yusen Lu
Velu, Govindan
Ruiqin Liu
Jia Liu
Xiangquan Wang
Dengcai Liu
Youliang Zheng
Lin Huang
Bihua Wu
Overexpression of TdNACB improves the drought resistance of rice
title Overexpression of TdNACB improves the drought resistance of rice
title_full Overexpression of TdNACB improves the drought resistance of rice
title_fullStr Overexpression of TdNACB improves the drought resistance of rice
title_full_unstemmed Overexpression of TdNACB improves the drought resistance of rice
title_short Overexpression of TdNACB improves the drought resistance of rice
title_sort overexpression of tdnacb improves the drought resistance of rice
topic drought
drought resistance
rice
wheat
yield losses
url https://hdl.handle.net/10568/162655
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