Allelic variation in Puroindoline genes in synthetic hexaploid wheats and development of allele-specific PCR markers for new soft-texture alleles
Grain hardness is an important end-use quality trait which can significantly affect milling and processing quality of wheat. It is controlled by Puroindoline (Pin) genes in wheat, of which Pina-D1 and Pinb-D1 control major phenotypic variation, while Pinb-B2 explain moderate variation. We analyzed t...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2024
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/162650 |
| _version_ | 1855526715251490816 |
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| author | Khan, Muhammad Aamir Yuanyuan Tian Humaira Qayyum Awais Rasheed He Zhonghu |
| author_browse | Awais Rasheed He Zhonghu Humaira Qayyum Khan, Muhammad Aamir Yuanyuan Tian |
| author_facet | Khan, Muhammad Aamir Yuanyuan Tian Humaira Qayyum Awais Rasheed He Zhonghu |
| author_sort | Khan, Muhammad Aamir |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Grain hardness is an important end-use quality trait which can significantly affect milling and processing quality of wheat. It is controlled by Puroindoline (Pin) genes in wheat, of which Pina-D1 and Pinb-D1 control major phenotypic variation, while Pinb-B2 explain moderate variation. We analyzed the allelic variation of three Pin genes in a panel of 227 synthetic hexaploid wheats (SHWs) using allele-specific markers either previously available or newly developed in this study to identify new alleles and facilitate their introgression. Eight different alleles were identified in Pina-D1 gene using six newly developed markers. The wild-type alleles, Pina-D1a and Pinb-D1a, were identified in 11.6 and 24.2% of accessions, respectively. It was found that Pina-D1c was most frequent (30.7%), followed by Pina-D1e allele (18%) at Pina-D1. For Pinb-D1, three new markers were developed which classified synthetic wheats into four haplotype groups, of which Hap-IV (Pinb-D1j/Pinb-D1o) was most frequent (52.8%). A cleaved amplified polymorphic site (CAPS) marker using ApoI restriction enzyme successfully classified accessions into wild-type and non-wild-type groups. At Pinb-B2 gene, the frequency of Pinb-B2b allele was higher (76.2%) which is associated with relatively hard texture compared to Pinb-B2a allele. The results provide a deeper insight into the allelic composition of grain of grain texture genes in SHWs which can be introgressed to develop required texture market class using allele-specific markers developed in this study. |
| format | Journal Article |
| id | CGSpace162650 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1626502025-10-26T12:51:40Z Allelic variation in Puroindoline genes in synthetic hexaploid wheats and development of allele-specific PCR markers for new soft-texture alleles Khan, Muhammad Aamir Yuanyuan Tian Humaira Qayyum Awais Rasheed He Zhonghu cold tolerance alleles genetic variation genetic markers introgression Grain hardness is an important end-use quality trait which can significantly affect milling and processing quality of wheat. It is controlled by Puroindoline (Pin) genes in wheat, of which Pina-D1 and Pinb-D1 control major phenotypic variation, while Pinb-B2 explain moderate variation. We analyzed the allelic variation of three Pin genes in a panel of 227 synthetic hexaploid wheats (SHWs) using allele-specific markers either previously available or newly developed in this study to identify new alleles and facilitate their introgression. Eight different alleles were identified in Pina-D1 gene using six newly developed markers. The wild-type alleles, Pina-D1a and Pinb-D1a, were identified in 11.6 and 24.2% of accessions, respectively. It was found that Pina-D1c was most frequent (30.7%), followed by Pina-D1e allele (18%) at Pina-D1. For Pinb-D1, three new markers were developed which classified synthetic wheats into four haplotype groups, of which Hap-IV (Pinb-D1j/Pinb-D1o) was most frequent (52.8%). A cleaved amplified polymorphic site (CAPS) marker using ApoI restriction enzyme successfully classified accessions into wild-type and non-wild-type groups. At Pinb-B2 gene, the frequency of Pinb-B2b allele was higher (76.2%) which is associated with relatively hard texture compared to Pinb-B2a allele. The results provide a deeper insight into the allelic composition of grain of grain texture genes in SHWs which can be introgressed to develop required texture market class using allele-specific markers developed in this study. 2024-12 2024-11-22T18:08:52Z 2024-11-22T18:08:52Z Journal Article https://hdl.handle.net/10568/162650 en Limited Access Springer Khan, M. A., Tian, Y., Qayyum, H., Rasheed, A., & He, Z. (2024). Allelic variation in Puroindoline genes in synthetic hexaploid wheats and development of allele-specific PCR markers for new soft-texture alleles. Cereal Research Communications. https://doi.org/10.1007/s42976-024-00497-4 |
| spellingShingle | cold tolerance alleles genetic variation genetic markers introgression Khan, Muhammad Aamir Yuanyuan Tian Humaira Qayyum Awais Rasheed He Zhonghu Allelic variation in Puroindoline genes in synthetic hexaploid wheats and development of allele-specific PCR markers for new soft-texture alleles |
| title | Allelic variation in Puroindoline genes in synthetic hexaploid wheats and development of allele-specific PCR markers for new soft-texture alleles |
| title_full | Allelic variation in Puroindoline genes in synthetic hexaploid wheats and development of allele-specific PCR markers for new soft-texture alleles |
| title_fullStr | Allelic variation in Puroindoline genes in synthetic hexaploid wheats and development of allele-specific PCR markers for new soft-texture alleles |
| title_full_unstemmed | Allelic variation in Puroindoline genes in synthetic hexaploid wheats and development of allele-specific PCR markers for new soft-texture alleles |
| title_short | Allelic variation in Puroindoline genes in synthetic hexaploid wheats and development of allele-specific PCR markers for new soft-texture alleles |
| title_sort | allelic variation in puroindoline genes in synthetic hexaploid wheats and development of allele specific pcr markers for new soft texture alleles |
| topic | cold tolerance alleles genetic variation genetic markers introgression |
| url | https://hdl.handle.net/10568/162650 |
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