Identification of QTL for alkylresorcinols in wheat and development of KASP markers for marker-assisted selection of health-promoting varieties
This study evaluated alkylresorcinol concentration (ARC) in recombinant inbred lines (RILs) from the cross of Zhongmai 578 and Jimai 22 in three environments. ARC exhibited a continuous distribution ranging from 337.4 to 758.0, 495.4–768.0, and 456.3–764.7 μg/g, respectively, in three environments....
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
American Chemical Society
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/162649 |
| _version_ | 1855531570904956928 |
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| author | Lei Zhi Xue Gong Hongyu Zhang Jindong Liu Shuanghe Cao Yong Zhang Jun Yan Wenfei Tian He Zhonghu |
| author_browse | He Zhonghu Hongyu Zhang Jindong Liu Jun Yan Lei Zhi Shuanghe Cao Wenfei Tian Xue Gong Yong Zhang |
| author_facet | Lei Zhi Xue Gong Hongyu Zhang Jindong Liu Shuanghe Cao Yong Zhang Jun Yan Wenfei Tian He Zhonghu |
| author_sort | Lei Zhi |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | This study evaluated alkylresorcinol concentration (ARC) in recombinant inbred lines (RILs) from the cross of Zhongmai 578 and Jimai 22 in three environments. ARC exhibited a continuous distribution ranging from 337.4 to 758.0, 495.4–768.0, and 456.3–764.7 μg/g, respectively, in three environments. Analysis of variance (ANOVA) indicated significant (P < 0.001) impacts of genotypes, environments, and their interactions. The broad-sense heritability of ARC was 0.76. Genome-wide linkage mapping analysis identified four stable quantitative trait loci (QTL) for ARC on chromosomes 2A, 3A, 4D, and 7A. Kompetitive allele-specific PCR (KASP) marker of each QTL was developed and validated in 206 representative wheat varieties. Wheat varieties harboring 0, 1, 2, 3, and 4 favorable alleles had ARC of 499.1, 587.8, 644.7, 668.5, and 711.1 μg/g, respectively. This study suggests that combining multiple minor-effect QTL through KASP markers can serve as an effective strategy for breeding high-ARC wheat, thereby enhancing innovations in functional food production. |
| format | Journal Article |
| id | CGSpace162649 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | American Chemical Society |
| publisherStr | American Chemical Society |
| record_format | dspace |
| spelling | CGSpace1626492025-10-26T12:56:05Z Identification of QTL for alkylresorcinols in wheat and development of KASP markers for marker-assisted selection of health-promoting varieties Lei Zhi Xue Gong Hongyu Zhang Jindong Liu Shuanghe Cao Yong Zhang Jun Yan Wenfei Tian He Zhonghu alkylresorcinols phytochemicals quantitative trait locus mapping marker-assisted selection wheat This study evaluated alkylresorcinol concentration (ARC) in recombinant inbred lines (RILs) from the cross of Zhongmai 578 and Jimai 22 in three environments. ARC exhibited a continuous distribution ranging from 337.4 to 758.0, 495.4–768.0, and 456.3–764.7 μg/g, respectively, in three environments. Analysis of variance (ANOVA) indicated significant (P < 0.001) impacts of genotypes, environments, and their interactions. The broad-sense heritability of ARC was 0.76. Genome-wide linkage mapping analysis identified four stable quantitative trait loci (QTL) for ARC on chromosomes 2A, 3A, 4D, and 7A. Kompetitive allele-specific PCR (KASP) marker of each QTL was developed and validated in 206 representative wheat varieties. Wheat varieties harboring 0, 1, 2, 3, and 4 favorable alleles had ARC of 499.1, 587.8, 644.7, 668.5, and 711.1 μg/g, respectively. This study suggests that combining multiple minor-effect QTL through KASP markers can serve as an effective strategy for breeding high-ARC wheat, thereby enhancing innovations in functional food production. 2024-08-07 2024-11-22T18:08:47Z 2024-11-22T18:08:47Z Journal Article https://hdl.handle.net/10568/162649 en Limited Access American Chemical Society Zhi, L., Gong, X., Zhang, H., Liu, J., Cao, S., Zhang, Y., Yan, J., Tian, W., & He, Z. (2024). Identification of QTL for Alkylresorcinols in Wheat and Development of KASP Markers for Marker-Assisted Selection of Health-Promoting Varieties. Journal of Agricultural and Food Chemistry, 72(31). https://doi.org/10.1021/acs.jafc.4c04674 |
| spellingShingle | alkylresorcinols phytochemicals quantitative trait locus mapping marker-assisted selection wheat Lei Zhi Xue Gong Hongyu Zhang Jindong Liu Shuanghe Cao Yong Zhang Jun Yan Wenfei Tian He Zhonghu Identification of QTL for alkylresorcinols in wheat and development of KASP markers for marker-assisted selection of health-promoting varieties |
| title | Identification of QTL for alkylresorcinols in wheat and development of KASP markers for marker-assisted selection of health-promoting varieties |
| title_full | Identification of QTL for alkylresorcinols in wheat and development of KASP markers for marker-assisted selection of health-promoting varieties |
| title_fullStr | Identification of QTL for alkylresorcinols in wheat and development of KASP markers for marker-assisted selection of health-promoting varieties |
| title_full_unstemmed | Identification of QTL for alkylresorcinols in wheat and development of KASP markers for marker-assisted selection of health-promoting varieties |
| title_short | Identification of QTL for alkylresorcinols in wheat and development of KASP markers for marker-assisted selection of health-promoting varieties |
| title_sort | identification of qtl for alkylresorcinols in wheat and development of kasp markers for marker assisted selection of health promoting varieties |
| topic | alkylresorcinols phytochemicals quantitative trait locus mapping marker-assisted selection wheat |
| url | https://hdl.handle.net/10568/162649 |
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