Molecular characterization of QTL for grain zinc and iron concentrations in wheat landrace Chinese Spring
Wheat is a major source of dietary energy for the growing world population. Developing cultivars with enriched zinc and iron can potentially alleviate human micronutrient deficiency. In this study, a recombinant inbred line (RIL) population with 245 lines derived from cross Zhou 8425B/Chinese Spring...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/162620 |
| _version_ | 1855518202794082304 |
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| author | Mengjing Sun Jingyang Tong Yan Dong Zongjun Pu Jianmin Zheng Yelun Zhang Xueyong Zhang Chenyang Hao Xiaowan Xu Qiang Cao Awais Rasheed Mohamed Badry Ali Shuanghe Cao Xianchun Xia He Zhonghu Zhongfu Ni Yuanfeng Hao |
| author_browse | Awais Rasheed Chenyang Hao He Zhonghu Jianmin Zheng Jingyang Tong Mengjing Sun Mohamed Badry Ali Qiang Cao Shuanghe Cao Xianchun Xia Xiaowan Xu Xueyong Zhang Yan Dong Yelun Zhang Yuanfeng Hao Zhongfu Ni Zongjun Pu |
| author_facet | Mengjing Sun Jingyang Tong Yan Dong Zongjun Pu Jianmin Zheng Yelun Zhang Xueyong Zhang Chenyang Hao Xiaowan Xu Qiang Cao Awais Rasheed Mohamed Badry Ali Shuanghe Cao Xianchun Xia He Zhonghu Zhongfu Ni Yuanfeng Hao |
| author_sort | Mengjing Sun |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Wheat is a major source of dietary energy for the growing world population. Developing cultivars with enriched zinc and iron can potentially alleviate human micronutrient deficiency. In this study, a recombinant inbred line (RIL) population with 245 lines derived from cross Zhou 8425B/Chinese Spring was used to detect quantitative trait loci (QTL) for grain zinc concentration (GZnC) and grain iron concentration (GFeC) across four environments. Three stable QTL for GZnC with all favorable alleles from Chinese Spring were identified on chromosomes 3BL, 5AL, and 5BL. These QTL explaining maxima of 8.7%, 5.8%, and 7.1% of phenotypic variances were validated in 125 resequenced wheat accessions encompassing both landraces and modern cultivars using six kompetitive allele specific PCR (KASP) assays. The frequencies of favorable alleles for QGZnCzc.caas-3BL, QGZnCzc.caas-5AL and QGZnCzc.caas-5BL were higher in landraces (90.4%, 68.0%, and 100.0%, respectively) compared to modern cultivars (45.9%, 35.4%, and 40.9%), suggesting they were not selected in breeding programs. Candidate gene association studies on GZnC in the cultivar panel further delimited the QTL into 8.5 Mb, 4.1 Mb, and 47.8 Mb regions containing 46, 4, and 199 candidate genes, respectively. The 5BL QTL located in a region where recombination was suppressed. Two stable and three less stable QTL for GFeC with favorable alleles also from Chinese Spring were identified on chromosomes 4BS (Rht-B1a), 4DS (Rht-D1a), 1DS, 3AS, and 6DS. This study sheds light on the genetic basis of GZnC and GFeC in Chinese Spring and provides useful molecular markers for wheat biofortification. |
| format | Journal Article |
| id | CGSpace162620 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1626202025-12-08T09:54:28Z Molecular characterization of QTL for grain zinc and iron concentrations in wheat landrace Chinese Spring Mengjing Sun Jingyang Tong Yan Dong Zongjun Pu Jianmin Zheng Yelun Zhang Xueyong Zhang Chenyang Hao Xiaowan Xu Qiang Cao Awais Rasheed Mohamed Badry Ali Shuanghe Cao Xianchun Xia He Zhonghu Zhongfu Ni Yuanfeng Hao molecular characterization quantitative trait loci wheat zinc iron field experimentation Wheat is a major source of dietary energy for the growing world population. Developing cultivars with enriched zinc and iron can potentially alleviate human micronutrient deficiency. In this study, a recombinant inbred line (RIL) population with 245 lines derived from cross Zhou 8425B/Chinese Spring was used to detect quantitative trait loci (QTL) for grain zinc concentration (GZnC) and grain iron concentration (GFeC) across four environments. Three stable QTL for GZnC with all favorable alleles from Chinese Spring were identified on chromosomes 3BL, 5AL, and 5BL. These QTL explaining maxima of 8.7%, 5.8%, and 7.1% of phenotypic variances were validated in 125 resequenced wheat accessions encompassing both landraces and modern cultivars using six kompetitive allele specific PCR (KASP) assays. The frequencies of favorable alleles for QGZnCzc.caas-3BL, QGZnCzc.caas-5AL and QGZnCzc.caas-5BL were higher in landraces (90.4%, 68.0%, and 100.0%, respectively) compared to modern cultivars (45.9%, 35.4%, and 40.9%), suggesting they were not selected in breeding programs. Candidate gene association studies on GZnC in the cultivar panel further delimited the QTL into 8.5 Mb, 4.1 Mb, and 47.8 Mb regions containing 46, 4, and 199 candidate genes, respectively. The 5BL QTL located in a region where recombination was suppressed. Two stable and three less stable QTL for GFeC with favorable alleles also from Chinese Spring were identified on chromosomes 4BS (Rht-B1a), 4DS (Rht-D1a), 1DS, 3AS, and 6DS. This study sheds light on the genetic basis of GZnC and GFeC in Chinese Spring and provides useful molecular markers for wheat biofortification. 2024-07 2024-11-22T14:41:19Z 2024-11-22T14:41:19Z Journal Article https://hdl.handle.net/10568/162620 en Limited Access Springer Sun, M., Tong, J., Dong, Y., Pu, Z., Zheng, J., Zhang, Y., Zhang, X., Hao, C., Xu, X., Cao, Q., Rasheed, A., Ali, M. B., Cao, S., Xia, X., He, Z., Ni, Z., & Hao, Y. (2024). Molecular characterization of QTL for grain zinc and iron concentrations in wheat landrace Chinese Spring. Theoretical and Applied Genetics, 137(7), 148. https://doi.org/10.1007/s00122-024-04661-6 |
| spellingShingle | molecular characterization quantitative trait loci wheat zinc iron field experimentation Mengjing Sun Jingyang Tong Yan Dong Zongjun Pu Jianmin Zheng Yelun Zhang Xueyong Zhang Chenyang Hao Xiaowan Xu Qiang Cao Awais Rasheed Mohamed Badry Ali Shuanghe Cao Xianchun Xia He Zhonghu Zhongfu Ni Yuanfeng Hao Molecular characterization of QTL for grain zinc and iron concentrations in wheat landrace Chinese Spring |
| title | Molecular characterization of QTL for grain zinc and iron concentrations in wheat landrace Chinese Spring |
| title_full | Molecular characterization of QTL for grain zinc and iron concentrations in wheat landrace Chinese Spring |
| title_fullStr | Molecular characterization of QTL for grain zinc and iron concentrations in wheat landrace Chinese Spring |
| title_full_unstemmed | Molecular characterization of QTL for grain zinc and iron concentrations in wheat landrace Chinese Spring |
| title_short | Molecular characterization of QTL for grain zinc and iron concentrations in wheat landrace Chinese Spring |
| title_sort | molecular characterization of qtl for grain zinc and iron concentrations in wheat landrace chinese spring |
| topic | molecular characterization quantitative trait loci wheat zinc iron field experimentation |
| url | https://hdl.handle.net/10568/162620 |
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