Stripe rust resistance gene Yr15 in Turkish and Kazakhstan wheat germplasms and the potential of Turkish wild emmer for stripe rust breeding
Puccinia striiformis f. sp. tritici (Pst) is a devastating obligate fungal biotroph that causes stripe rust disease in wheat in temperate and cooler regions, especially in Kazakhstan and Turkey. Chemical control of Pst is not a sustainable or economic strategy in these countries. Therefore, a suitab...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2024
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/162619 |
| _version_ | 1855540617743958016 |
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| author | Baloch, Faheem Shehzad Ali, Amjad Tajibayev, Daniyar Nadeem, Muhammad Azhar Ölmez, Fatih Aktas, Hüsnü Alsaleh, Ahmad Cömertpay, Gönül Imren, Mustafa Mustafa, Zemran Dababat, Abdelfattah A. Yang, Seung Hwan |
| author_browse | Aktas, Hüsnü Ali, Amjad Alsaleh, Ahmad Baloch, Faheem Shehzad Cömertpay, Gönül Dababat, Abdelfattah A. Imren, Mustafa Mustafa, Zemran Nadeem, Muhammad Azhar Tajibayev, Daniyar Yang, Seung Hwan Ölmez, Fatih |
| author_facet | Baloch, Faheem Shehzad Ali, Amjad Tajibayev, Daniyar Nadeem, Muhammad Azhar Ölmez, Fatih Aktas, Hüsnü Alsaleh, Ahmad Cömertpay, Gönül Imren, Mustafa Mustafa, Zemran Dababat, Abdelfattah A. Yang, Seung Hwan |
| author_sort | Baloch, Faheem Shehzad |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Puccinia striiformis f. sp. tritici (Pst) is a devastating obligate fungal biotroph that causes stripe rust disease in wheat in temperate and cooler regions, especially in Kazakhstan and Turkey. Chemical control of Pst is not a sustainable or economic strategy in these countries. Therefore, a suitable alternative to these pesticides, such as the development of resistant wheat cultivars, is needed. During the present investigation, a large set of wheat germplasms was characterized to determine the presence of the broad-spectrum resistance Yr15 gene using Y15K1_F2/uhw30_1R primers. This collection contained 360 accessions including 233 durum and bread wheat collected from KASIB cultivars and advanced lines that were developed by eight breeding programs within the Kazakhstan-Siberia Wheat Improvement Network and Turkey, and 127 wild emmer collected from Turkey only. The positive control was wild emmer IDs WEW 83-1, WEW 84-1, and WEW-85, which previously showed positive amplification of the Yr15 gene with these primers. Yr15, a broad-spectrum R-gene derived from wild emmer wheat encoding a putative kinase-pseudokinase protein designated as wheat tandem kinase 1, comprises a unique R-gene structure in wheat against the yellow (stripe) rust. None of the tested cultivated wheat germplasms from 174 durum wheat of the KASIB Network or 59 of bread wheat from Turkey showed positive amplification, but 89 of 127 (70.07%) wild Triticum dicoccoides showed positive amplification. The collection from the Karacadağ regions showed the maximum number of genotypes (46) with positive amplification for Yr15, followed by Eruh (10), Şırnak (5), Malazgir-Tunceli (4), Tunceli (4), Şırnak-Uludere yol (3), Tunceli-Pülümür (3), and Karacadağ/Kerteş Köyü –Siverek (3). We conclude that wild wheat cultivars are a good source of the Yr15 gene and that introgression of this gene into the cultivated wheat germplasm of both countries should be performed to manage the Pst pathogen in the future. |
| format | Journal Article |
| id | CGSpace162619 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1626192025-12-08T10:11:39Z Stripe rust resistance gene Yr15 in Turkish and Kazakhstan wheat germplasms and the potential of Turkish wild emmer for stripe rust breeding Baloch, Faheem Shehzad Ali, Amjad Tajibayev, Daniyar Nadeem, Muhammad Azhar Ölmez, Fatih Aktas, Hüsnü Alsaleh, Ahmad Cömertpay, Gönül Imren, Mustafa Mustafa, Zemran Dababat, Abdelfattah A. Yang, Seung Hwan stripe rust resistance genes wheat germplasm Puccinia striiformis f. sp. tritici (Pst) is a devastating obligate fungal biotroph that causes stripe rust disease in wheat in temperate and cooler regions, especially in Kazakhstan and Turkey. Chemical control of Pst is not a sustainable or economic strategy in these countries. Therefore, a suitable alternative to these pesticides, such as the development of resistant wheat cultivars, is needed. During the present investigation, a large set of wheat germplasms was characterized to determine the presence of the broad-spectrum resistance Yr15 gene using Y15K1_F2/uhw30_1R primers. This collection contained 360 accessions including 233 durum and bread wheat collected from KASIB cultivars and advanced lines that were developed by eight breeding programs within the Kazakhstan-Siberia Wheat Improvement Network and Turkey, and 127 wild emmer collected from Turkey only. The positive control was wild emmer IDs WEW 83-1, WEW 84-1, and WEW-85, which previously showed positive amplification of the Yr15 gene with these primers. Yr15, a broad-spectrum R-gene derived from wild emmer wheat encoding a putative kinase-pseudokinase protein designated as wheat tandem kinase 1, comprises a unique R-gene structure in wheat against the yellow (stripe) rust. None of the tested cultivated wheat germplasms from 174 durum wheat of the KASIB Network or 59 of bread wheat from Turkey showed positive amplification, but 89 of 127 (70.07%) wild Triticum dicoccoides showed positive amplification. The collection from the Karacadağ regions showed the maximum number of genotypes (46) with positive amplification for Yr15, followed by Eruh (10), Şırnak (5), Malazgir-Tunceli (4), Tunceli (4), Şırnak-Uludere yol (3), Tunceli-Pülümür (3), and Karacadağ/Kerteş Köyü –Siverek (3). We conclude that wild wheat cultivars are a good source of the Yr15 gene and that introgression of this gene into the cultivated wheat germplasm of both countries should be performed to manage the Pst pathogen in the future. 2024-08 2024-11-22T14:37:27Z 2024-11-22T14:37:27Z Journal Article https://hdl.handle.net/10568/162619 en Limited Access Springer Baloch, F. S., Ali, A., Tajibayev, D., Nadeem, M. A., Ölmez, F., Aktaş, H., Alsaleh, A., Cömertpay, G., Imren, M., Mustafa, Z., Dababat, A. A., & Yang, S. H. (2024). Stripe rust resistance gene Yr15 in Turkish and Kazakhstan wheat germplasms and the potential of Turkish wild emmer for stripe rust breeding. Genetic Resources and Crop Evolution, 71(6), 2699–2719. https://doi.org/10.1007/s10722-023-01804-4 |
| spellingShingle | stripe rust resistance genes wheat germplasm Baloch, Faheem Shehzad Ali, Amjad Tajibayev, Daniyar Nadeem, Muhammad Azhar Ölmez, Fatih Aktas, Hüsnü Alsaleh, Ahmad Cömertpay, Gönül Imren, Mustafa Mustafa, Zemran Dababat, Abdelfattah A. Yang, Seung Hwan Stripe rust resistance gene Yr15 in Turkish and Kazakhstan wheat germplasms and the potential of Turkish wild emmer for stripe rust breeding |
| title | Stripe rust resistance gene Yr15 in Turkish and Kazakhstan wheat germplasms and the potential of Turkish wild emmer for stripe rust breeding |
| title_full | Stripe rust resistance gene Yr15 in Turkish and Kazakhstan wheat germplasms and the potential of Turkish wild emmer for stripe rust breeding |
| title_fullStr | Stripe rust resistance gene Yr15 in Turkish and Kazakhstan wheat germplasms and the potential of Turkish wild emmer for stripe rust breeding |
| title_full_unstemmed | Stripe rust resistance gene Yr15 in Turkish and Kazakhstan wheat germplasms and the potential of Turkish wild emmer for stripe rust breeding |
| title_short | Stripe rust resistance gene Yr15 in Turkish and Kazakhstan wheat germplasms and the potential of Turkish wild emmer for stripe rust breeding |
| title_sort | stripe rust resistance gene yr15 in turkish and kazakhstan wheat germplasms and the potential of turkish wild emmer for stripe rust breeding |
| topic | stripe rust resistance genes wheat germplasm |
| url | https://hdl.handle.net/10568/162619 |
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