Targeted improvement of plant-based protein: Genome-wide association mapping of a lentil (Lens culinaris Medik.) diversity panel
The world is increasingly looking to plant-based sources to meet its protein needs.Multiple factors are driving this progression, ranging from nutritional and ethical con-siderations to climate change and population growth. As a pulse crop, lentil is ideal tohelp meet this change in demand. However,...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Wiley Open Access
2024
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| Acceso en línea: | https://hdl.handle.net/10568/162584 |
| _version_ | 1855524492441288704 |
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| author | Johnson, Nathan Boatwright, Jon Lucas Bridges, William Thavarajah, Pushparajah Agrawal, Shiv Kumar Thavarajah, Dil |
| author_browse | Agrawal, Shiv Kumar Boatwright, Jon Lucas Bridges, William Johnson, Nathan Thavarajah, Dil Thavarajah, Pushparajah |
| author_facet | Johnson, Nathan Boatwright, Jon Lucas Bridges, William Thavarajah, Pushparajah Agrawal, Shiv Kumar Thavarajah, Dil |
| author_sort | Johnson, Nathan |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The world is increasingly looking to plant-based sources to meet its protein needs.Multiple factors are driving this progression, ranging from nutritional and ethical con-siderations to climate change and population growth. As a pulse crop, lentil is ideal tohelp meet this change in demand. However, plant-based proteins have limiting aminoacids and lower protein digestibility compared to animal-based proteins. Thisresearch identifies genetic markers that can be used to accelerate breeding of proteinquality traits in lentil to ultimately help meet the rising demand in high-quality plant-based protein and bolster global food and nutritional security.Summary• Lentil (Lens culinaris Medik.) contains 25% high-quality protein in addition to highconcentrations of prebiotic carbohydrates and micronutrients, such as folate, iron,zinc, and selenium. As animal-based protein's economic and environmental costsrise, plant-based protein sources, such as lentil, will become increasingly importantto global food systems. Consequently, evaluating and targeting protein quality traitsfor genomic-assisted breeding is a valuable objective for lentil breeding programs.• A diversity panel of 183 breeding lines was analyzed for protein quality traits, includ-ing amino acids and protein digestibility. Genotyping-by-sequencing (GBS) data wereused to assess population structure and conduct genome-wide association studies(GWAS). Genes in local linkage disequilibrium (LD) with significant single nucleotidepolymorphism (SNP) markers were identified and categorized by homology.• Protein quality traits showed a wide range of variation. Repeatability estimateswere low to moderate across traits. Twelve traits were strongly correlated witheach other (r > .7). Admixture analysis identified six ancestral subpopulations,which also demonstrated clustering in principal component analysis. Ten differenttraits had significant SNP associations; two loci were shared across multiple traits.Twenty-seven candidate genes, including glutathione S-transferase, protease fam-ily, and gibberellin 2-beta-dioxygenase genes, were identified. |
| format | Journal Article |
| id | CGSpace162584 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Wiley Open Access |
| publisherStr | Wiley Open Access |
| record_format | dspace |
| spelling | CGSpace1625842026-01-14T02:07:24Z Targeted improvement of plant-based protein: Genome-wide association mapping of a lentil (Lens culinaris Medik.) diversity panel Johnson, Nathan Boatwright, Jon Lucas Bridges, William Thavarajah, Pushparajah Agrawal, Shiv Kumar Thavarajah, Dil breeding pulses amino acids lentil genome-wide association study admixture lentil protein protein digestibility The world is increasingly looking to plant-based sources to meet its protein needs.Multiple factors are driving this progression, ranging from nutritional and ethical con-siderations to climate change and population growth. As a pulse crop, lentil is ideal tohelp meet this change in demand. However, plant-based proteins have limiting aminoacids and lower protein digestibility compared to animal-based proteins. Thisresearch identifies genetic markers that can be used to accelerate breeding of proteinquality traits in lentil to ultimately help meet the rising demand in high-quality plant-based protein and bolster global food and nutritional security.Summary• Lentil (Lens culinaris Medik.) contains 25% high-quality protein in addition to highconcentrations of prebiotic carbohydrates and micronutrients, such as folate, iron,zinc, and selenium. As animal-based protein's economic and environmental costsrise, plant-based protein sources, such as lentil, will become increasingly importantto global food systems. Consequently, evaluating and targeting protein quality traitsfor genomic-assisted breeding is a valuable objective for lentil breeding programs.• A diversity panel of 183 breeding lines was analyzed for protein quality traits, includ-ing amino acids and protein digestibility. Genotyping-by-sequencing (GBS) data wereused to assess population structure and conduct genome-wide association studies(GWAS). Genes in local linkage disequilibrium (LD) with significant single nucleotidepolymorphism (SNP) markers were identified and categorized by homology.• Protein quality traits showed a wide range of variation. Repeatability estimateswere low to moderate across traits. Twelve traits were strongly correlated witheach other (r > .7). Admixture analysis identified six ancestral subpopulations,which also demonstrated clustering in principal component analysis. Ten differenttraits had significant SNP associations; two loci were shared across multiple traits.Twenty-seven candidate genes, including glutathione S-transferase, protease fam-ily, and gibberellin 2-beta-dioxygenase genes, were identified. 2024-05-01 2024-11-21T22:16:41Z 2024-11-21T22:16:41Z Journal Article https://hdl.handle.net/10568/162584 en Open Access application/pdf Wiley Open Access Nathan Johnson, Jon Lucas Boatwright, William Bridges, Pushparajah Thavarajah, Shiv Kumar Agrawal, Dil Thavarajah. (1/5/2024). Targeted improvement of plant-based protein: Genome-wide association mapping of a lentil (Lens culinaris Medik. ) diversity panel. Plants People Planet, 6 (3), pp. 640-655. |
| spellingShingle | breeding pulses amino acids lentil genome-wide association study admixture lentil protein protein digestibility Johnson, Nathan Boatwright, Jon Lucas Bridges, William Thavarajah, Pushparajah Agrawal, Shiv Kumar Thavarajah, Dil Targeted improvement of plant-based protein: Genome-wide association mapping of a lentil (Lens culinaris Medik.) diversity panel |
| title | Targeted improvement of plant-based protein: Genome-wide association mapping of a lentil (Lens culinaris Medik.) diversity panel |
| title_full | Targeted improvement of plant-based protein: Genome-wide association mapping of a lentil (Lens culinaris Medik.) diversity panel |
| title_fullStr | Targeted improvement of plant-based protein: Genome-wide association mapping of a lentil (Lens culinaris Medik.) diversity panel |
| title_full_unstemmed | Targeted improvement of plant-based protein: Genome-wide association mapping of a lentil (Lens culinaris Medik.) diversity panel |
| title_short | Targeted improvement of plant-based protein: Genome-wide association mapping of a lentil (Lens culinaris Medik.) diversity panel |
| title_sort | targeted improvement of plant based protein genome wide association mapping of a lentil lens culinaris medik diversity panel |
| topic | breeding pulses amino acids lentil genome-wide association study admixture lentil protein protein digestibility |
| url | https://hdl.handle.net/10568/162584 |
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