Polyamines mediate the inhibitory effect of drought stress on nitrogen reallocation and utilization to regulate grain number in wheat
Drought stress poses a serious threat to grain formation in wheat. Nitrogen (N) plays crucial roles in plant organ development; however, the physiological mechanisms by which drought stress affects plant N availability and mediates the formation of grains in spikes of winter wheat are still unclear....
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Oxford University Press (OUP): Policy F - Oxford Open Option D
2024
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/162578 |
| _version_ | 1855538361726402560 |
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| author | Li, Juan Li, Qi Nian, GUO Xian, Qinglin Lan, Bing Nangia, Vinay Mo, Fei Liu, Yang |
| author_browse | Lan, Bing Li, Juan Li, Qi Liu, Yang Mo, Fei Nangia, Vinay Nian, GUO Xian, Qinglin |
| author_facet | Li, Juan Li, Qi Nian, GUO Xian, Qinglin Lan, Bing Nangia, Vinay Mo, Fei Liu, Yang |
| author_sort | Li, Juan |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Drought stress poses a serious threat to grain formation in wheat. Nitrogen (N) plays crucial roles in plant organ development; however, the physiological mechanisms by which drought stress affects plant N availability and mediates the formation of grains in spikes of winter wheat are still unclear. In this study, we determined that pre-reproductive drought stress significantly reduced the number of fertile florets and the number of grains formed. Transcriptome analysis demonstrated that this was related to N metabolism, and in particular, the metabolism pathways of arginine (the main precursor for synthesis of polyamine) and proline. Continuous drought stress restricted plant N accumulation and reallocation rates, and plants preferentially allocated more N to spike development. As the activities of amino acid biosynthesis enzymes and catabolic enzymes were inhibited, more free amino acids accumulated in young spikes. The expression of polyamine synthase genes was down-regulated under drought stress, whilst expression of genes encoding catabolic enzymes was enhanced, resulting in reductions in endogenous spermidine and putrescine. Treatment with exogenous spermidine optimized N allocation in young spikes and leaves, which greatly alleviated the drought-induced reduction in the number of grains per spike. Overall, our results show that pre-reproductive drought stress affects wheat grain numbers by regulating N redistribution and polyamine metabolism. |
| format | Journal Article |
| id | CGSpace162578 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Oxford University Press (OUP): Policy F - Oxford Open Option D |
| publisherStr | Oxford University Press (OUP): Policy F - Oxford Open Option D |
| record_format | dspace |
| spelling | CGSpace1625782026-01-14T05:35:21Z Polyamines mediate the inhibitory effect of drought stress on nitrogen reallocation and utilization to regulate grain number in wheat Li, Juan Li, Qi Nian, GUO Xian, Qinglin Lan, Bing Nangia, Vinay Mo, Fei Liu, Yang wheat nitrogen use efficiency drought stress triticum aestivum spermidine wheat water deficit rna-seq grain yield amino acid fertile floret nitrogen allocation Drought stress poses a serious threat to grain formation in wheat. Nitrogen (N) plays crucial roles in plant organ development; however, the physiological mechanisms by which drought stress affects plant N availability and mediates the formation of grains in spikes of winter wheat are still unclear. In this study, we determined that pre-reproductive drought stress significantly reduced the number of fertile florets and the number of grains formed. Transcriptome analysis demonstrated that this was related to N metabolism, and in particular, the metabolism pathways of arginine (the main precursor for synthesis of polyamine) and proline. Continuous drought stress restricted plant N accumulation and reallocation rates, and plants preferentially allocated more N to spike development. As the activities of amino acid biosynthesis enzymes and catabolic enzymes were inhibited, more free amino acids accumulated in young spikes. The expression of polyamine synthase genes was down-regulated under drought stress, whilst expression of genes encoding catabolic enzymes was enhanced, resulting in reductions in endogenous spermidine and putrescine. Treatment with exogenous spermidine optimized N allocation in young spikes and leaves, which greatly alleviated the drought-induced reduction in the number of grains per spike. Overall, our results show that pre-reproductive drought stress affects wheat grain numbers by regulating N redistribution and polyamine metabolism. 2024-02-02 2024-11-21T21:53:02Z 2024-11-21T21:53:02Z Journal Article https://hdl.handle.net/10568/162578 en Limited Access Oxford University Press (OUP): Policy F - Oxford Open Option D Juan Li, Qi Li, GUO Nian, Qinglin Xian, Bing Lan, Vinay Nangia, Fei Mo, Yang Liu. (2/2/2024). Polyamines mediate the inhibitory effect of drought stress on nitrogen reallocation and utilization to regulate grain number in wheat. Journal of Experimental Botany, 75 (3), pp. 1016-1035. |
| spellingShingle | wheat nitrogen use efficiency drought stress triticum aestivum spermidine wheat water deficit rna-seq grain yield amino acid fertile floret nitrogen allocation Li, Juan Li, Qi Nian, GUO Xian, Qinglin Lan, Bing Nangia, Vinay Mo, Fei Liu, Yang Polyamines mediate the inhibitory effect of drought stress on nitrogen reallocation and utilization to regulate grain number in wheat |
| title | Polyamines mediate the inhibitory effect of drought stress on nitrogen reallocation and utilization to regulate grain number in wheat |
| title_full | Polyamines mediate the inhibitory effect of drought stress on nitrogen reallocation and utilization to regulate grain number in wheat |
| title_fullStr | Polyamines mediate the inhibitory effect of drought stress on nitrogen reallocation and utilization to regulate grain number in wheat |
| title_full_unstemmed | Polyamines mediate the inhibitory effect of drought stress on nitrogen reallocation and utilization to regulate grain number in wheat |
| title_short | Polyamines mediate the inhibitory effect of drought stress on nitrogen reallocation and utilization to regulate grain number in wheat |
| title_sort | polyamines mediate the inhibitory effect of drought stress on nitrogen reallocation and utilization to regulate grain number in wheat |
| topic | wheat nitrogen use efficiency drought stress triticum aestivum spermidine wheat water deficit rna-seq grain yield amino acid fertile floret nitrogen allocation |
| url | https://hdl.handle.net/10568/162578 |
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