Genetic mapping of stripe rust resistance in a geographically diverse barley collection and selected biparental populations
Barley stripe or yellow rust (BYR) caused by Puccinia striiformis f. sp. hordei (Psh) is a significant constraint to barley production. The disease is best controlled by genetic resistance, which is considered the most economical and sustainable component of integrated disease management. In this st...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Frontiers Media
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/162574 |
| _version_ | 1855519087778594816 |
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| author | Singh, Davinder Ziems, Laura Chettri, Mumta Dracatos, Peter Forrest, Kerrie Bhavani, Sridhar Singh, Ravi Barnes, Charles W. Noroña Zapata, Patricio Javier Gangwar, Om Kumar, Subodh Bhardwaj, Subhash Park, Robert F. |
| author_browse | Barnes, Charles W. Bhardwaj, Subhash Bhavani, Sridhar Chettri, Mumta Dracatos, Peter Forrest, Kerrie Gangwar, Om Kumar, Subodh Noroña Zapata, Patricio Javier Park, Robert F. Singh, Davinder Singh, Ravi Ziems, Laura |
| author_facet | Singh, Davinder Ziems, Laura Chettri, Mumta Dracatos, Peter Forrest, Kerrie Bhavani, Sridhar Singh, Ravi Barnes, Charles W. Noroña Zapata, Patricio Javier Gangwar, Om Kumar, Subodh Bhardwaj, Subhash Park, Robert F. |
| author_sort | Singh, Davinder |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Barley stripe or yellow rust (BYR) caused by Puccinia striiformis f. sp. hordei (Psh) is a significant constraint to barley production. The disease is best controlled by genetic resistance, which is considered the most economical and sustainable component of integrated disease management. In this study, we assessed the diversity of resistance to Psh in a panel of international barley genotypes (n = 266) under multiple disease environments (Ecuador, India, and Mexico) using genome-wide association studies (GWASs). Four quantitative trait loci (QTLs) (three on chromosome 1H and one on 7H) associated with resistance to Psh were identified. The QTLs were validated by mapping resistance to Psh in five biparental populations, which detected key genomic regions on chromosomes 1H (populations Pompadour/Zhoungdamei, Pompadour/Zug161, and CI9214/Baudin), 3H (Ricardo/Gus), and 7H (Fumai8/Baronesse). The QTL RpshQ.GWA.1H.1 detected by GWAS and RpshQ.Bau.1H detected using biparental mapping populations co-located were the most consistent and stable across environments and are likely the same resistance region. RpshQ.Bau.1H was saturated using population CI9214/Baudin by enriching the target region, which placed the resistance locus between 7.9 and 8.1 Mbp (flanked by markers sun_B1H_03, 0.7 cM proximal to Rpsh_1H and sun_B1H_KASP_02, 3.2 cM distal on 1HS) in the Morex reference genome v.2. A Kompetitive Allele Specific PCR (KASP) marker sun_B1H_KASP_01 that co-segregated for RpshQ.Bau.1H was developed. The marker was validated on 50 Australian barley cultivars, showing well-defined allelic discrimination and presence in six genotypes (Baudin, Fathom, Flagship, Grout, Sakurastar, and Shepherd). This marker can be used for reliable marker-assisted selection and pyramiding of resistance to Psh and in diversifying the genetic base of resistance to stripe rust. |
| format | Journal Article |
| id | CGSpace162574 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1625742025-12-08T10:29:22Z Genetic mapping of stripe rust resistance in a geographically diverse barley collection and selected biparental populations Singh, Davinder Ziems, Laura Chettri, Mumta Dracatos, Peter Forrest, Kerrie Bhavani, Sridhar Singh, Ravi Barnes, Charles W. Noroña Zapata, Patricio Javier Gangwar, Om Kumar, Subodh Bhardwaj, Subhash Park, Robert F. barley stripe rust puccinia striiformis genome-wide association studies association mapping quantitative trait loci marker-assisted selection Barley stripe or yellow rust (BYR) caused by Puccinia striiformis f. sp. hordei (Psh) is a significant constraint to barley production. The disease is best controlled by genetic resistance, which is considered the most economical and sustainable component of integrated disease management. In this study, we assessed the diversity of resistance to Psh in a panel of international barley genotypes (n = 266) under multiple disease environments (Ecuador, India, and Mexico) using genome-wide association studies (GWASs). Four quantitative trait loci (QTLs) (three on chromosome 1H and one on 7H) associated with resistance to Psh were identified. The QTLs were validated by mapping resistance to Psh in five biparental populations, which detected key genomic regions on chromosomes 1H (populations Pompadour/Zhoungdamei, Pompadour/Zug161, and CI9214/Baudin), 3H (Ricardo/Gus), and 7H (Fumai8/Baronesse). The QTL RpshQ.GWA.1H.1 detected by GWAS and RpshQ.Bau.1H detected using biparental mapping populations co-located were the most consistent and stable across environments and are likely the same resistance region. RpshQ.Bau.1H was saturated using population CI9214/Baudin by enriching the target region, which placed the resistance locus between 7.9 and 8.1 Mbp (flanked by markers sun_B1H_03, 0.7 cM proximal to Rpsh_1H and sun_B1H_KASP_02, 3.2 cM distal on 1HS) in the Morex reference genome v.2. A Kompetitive Allele Specific PCR (KASP) marker sun_B1H_KASP_01 that co-segregated for RpshQ.Bau.1H was developed. The marker was validated on 50 Australian barley cultivars, showing well-defined allelic discrimination and presence in six genotypes (Baudin, Fathom, Flagship, Grout, Sakurastar, and Shepherd). This marker can be used for reliable marker-assisted selection and pyramiding of resistance to Psh and in diversifying the genetic base of resistance to stripe rust. 2024-07 2024-11-21T21:40:27Z 2024-11-21T21:40:27Z Journal Article https://hdl.handle.net/10568/162574 en Open Access application/pdf Frontiers Media Singh, D., Ziems, L., Chettri, M., Dracatos, P. M., Forrest, K. L., Bhavani, S., Singh, R. P., Barnes, C. W., Noroña Zapata, P. J., Gangwar, O., Kumar, S., Bhardwaj, S., & Park, R. F. (2024). Genetic mapping of stripe rust resistance in a geographically diverse barley collection and selected biparental populations. Frontiers in Plant Science, 15, 1352402. https://doi.org/10.3389/fpls.2024.1352402 |
| spellingShingle | barley stripe rust puccinia striiformis genome-wide association studies association mapping quantitative trait loci marker-assisted selection Singh, Davinder Ziems, Laura Chettri, Mumta Dracatos, Peter Forrest, Kerrie Bhavani, Sridhar Singh, Ravi Barnes, Charles W. Noroña Zapata, Patricio Javier Gangwar, Om Kumar, Subodh Bhardwaj, Subhash Park, Robert F. Genetic mapping of stripe rust resistance in a geographically diverse barley collection and selected biparental populations |
| title | Genetic mapping of stripe rust resistance in a geographically diverse barley collection and selected biparental populations |
| title_full | Genetic mapping of stripe rust resistance in a geographically diverse barley collection and selected biparental populations |
| title_fullStr | Genetic mapping of stripe rust resistance in a geographically diverse barley collection and selected biparental populations |
| title_full_unstemmed | Genetic mapping of stripe rust resistance in a geographically diverse barley collection and selected biparental populations |
| title_short | Genetic mapping of stripe rust resistance in a geographically diverse barley collection and selected biparental populations |
| title_sort | genetic mapping of stripe rust resistance in a geographically diverse barley collection and selected biparental populations |
| topic | barley stripe rust puccinia striiformis genome-wide association studies association mapping quantitative trait loci marker-assisted selection |
| url | https://hdl.handle.net/10568/162574 |
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