Maize insensitivity to culture filtrates from Exserohilum turcicum is related to quantitative resistance
Turcicum leaf blight (TLB) of maize is caused by Exserohilum turcicum. The TLB resistance is mainly associated with qualitative race-specific resistance that is linked to several Ht genes namely Ht1, Ht2, Ht3, HtM, HtN, HtNB and HtP. However, quantitative TLB resistance also occurs, but its mechanis...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/162553 |
| _version_ | 1855540832948453376 |
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| author | Muñoz-Zavala, Carlos Goodwin, Paul Leyva Mir, Santos G. Loladze, Alexander Vargas-Hernández, Mateo García‑León, Elizabeth Tovar-Pedraza, Juan M. San Vicente, Félix M. Alakonya, Amos |
| author_browse | Alakonya, Amos García‑León, Elizabeth Goodwin, Paul Leyva Mir, Santos G. Loladze, Alexander Muñoz-Zavala, Carlos San Vicente, Félix M. Tovar-Pedraza, Juan M. Vargas-Hernández, Mateo |
| author_facet | Muñoz-Zavala, Carlos Goodwin, Paul Leyva Mir, Santos G. Loladze, Alexander Vargas-Hernández, Mateo García‑León, Elizabeth Tovar-Pedraza, Juan M. San Vicente, Félix M. Alakonya, Amos |
| author_sort | Muñoz-Zavala, Carlos |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Turcicum leaf blight (TLB) of maize is caused by Exserohilum turcicum. The TLB resistance is mainly associated with qualitative race-specific resistance that is linked to several Ht genes namely Ht1, Ht2, Ht3, HtM, HtN, HtNB and HtP. However, quantitative TLB resistance also occurs, but its mechanisms are poorly understood. In this study, tolerance or insensitivity to chlorosis and necrosis of E. turcicum culture filtrate was associated with quantitative TLB resistance. A novel detached maize seedling assay was developed for E. turcicum culture filtrate using methanol-treated modified Fries medium. Screening of E. turcicum isolates of races 2, 3, 23, 3 N, 23 N and 123 N against 61 maize inbred lines with diverse levels of resistance to TLB was conducted in the greenhouse by fungal inoculation and the detached seedling culture filtrate assay. Using an isolate of race 123 N, which can overcome all the qualitative resistance genes, a simple linear regression model (R-2 = 0.68, p = .90) for the 61 inbred lines was established between disease severity from foliar inoculations and culture filtrate symptom rating of the detached seedling assay. Two maize lines CML474 and CML483 had relatively high culture filtrate symptom ratings but very low disease severity with foliar inoculation indicating that these sources of quantitative TLB resistance may be unrelated to tolerance to E. turcicum culture filtrates, whose effect on seedlings could be due to the toxin known as HT-toxin or monocerin. |
| format | Journal Article |
| id | CGSpace162553 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1625532025-10-26T12:50:35Z Maize insensitivity to culture filtrates from Exserohilum turcicum is related to quantitative resistance Muñoz-Zavala, Carlos Goodwin, Paul Leyva Mir, Santos G. Loladze, Alexander Vargas-Hernández, Mateo García‑León, Elizabeth Tovar-Pedraza, Juan M. San Vicente, Félix M. Alakonya, Amos toxins in vitro bio-assays blight setosphaeria turcica Turcicum leaf blight (TLB) of maize is caused by Exserohilum turcicum. The TLB resistance is mainly associated with qualitative race-specific resistance that is linked to several Ht genes namely Ht1, Ht2, Ht3, HtM, HtN, HtNB and HtP. However, quantitative TLB resistance also occurs, but its mechanisms are poorly understood. In this study, tolerance or insensitivity to chlorosis and necrosis of E. turcicum culture filtrate was associated with quantitative TLB resistance. A novel detached maize seedling assay was developed for E. turcicum culture filtrate using methanol-treated modified Fries medium. Screening of E. turcicum isolates of races 2, 3, 23, 3 N, 23 N and 123 N against 61 maize inbred lines with diverse levels of resistance to TLB was conducted in the greenhouse by fungal inoculation and the detached seedling culture filtrate assay. Using an isolate of race 123 N, which can overcome all the qualitative resistance genes, a simple linear regression model (R-2 = 0.68, p = .90) for the 61 inbred lines was established between disease severity from foliar inoculations and culture filtrate symptom rating of the detached seedling assay. Two maize lines CML474 and CML483 had relatively high culture filtrate symptom ratings but very low disease severity with foliar inoculation indicating that these sources of quantitative TLB resistance may be unrelated to tolerance to E. turcicum culture filtrates, whose effect on seedlings could be due to the toxin known as HT-toxin or monocerin. 2024-07 2024-11-21T16:43:40Z 2024-11-21T16:43:40Z Journal Article https://hdl.handle.net/10568/162553 en Open Access application/pdf Wiley Muñoz‐Zavala, C., Goodwin, P. H., Leyva‐Mir, S. G., Loladze, A., Vargas‐Hernández, M., García‐León, E., Tovar‐Pedraza, J. M., San Vicente, F. M., & Alakonya, A. (2024). Maize insensitivity to culture filtrates from Exserohilum turcicum is related to quantitative resistance. Journal of Phytopathology, 172(4), e13350. https://doi.org/10.1111/jph.13350 |
| spellingShingle | toxins in vitro bio-assays blight setosphaeria turcica Muñoz-Zavala, Carlos Goodwin, Paul Leyva Mir, Santos G. Loladze, Alexander Vargas-Hernández, Mateo García‑León, Elizabeth Tovar-Pedraza, Juan M. San Vicente, Félix M. Alakonya, Amos Maize insensitivity to culture filtrates from Exserohilum turcicum is related to quantitative resistance |
| title | Maize insensitivity to culture filtrates from Exserohilum turcicum is related to quantitative resistance |
| title_full | Maize insensitivity to culture filtrates from Exserohilum turcicum is related to quantitative resistance |
| title_fullStr | Maize insensitivity to culture filtrates from Exserohilum turcicum is related to quantitative resistance |
| title_full_unstemmed | Maize insensitivity to culture filtrates from Exserohilum turcicum is related to quantitative resistance |
| title_short | Maize insensitivity to culture filtrates from Exserohilum turcicum is related to quantitative resistance |
| title_sort | maize insensitivity to culture filtrates from exserohilum turcicum is related to quantitative resistance |
| topic | toxins in vitro bio-assays blight setosphaeria turcica |
| url | https://hdl.handle.net/10568/162553 |
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