Genomic insights of a native bacterial consortium for wheat production sustainability
The use of plant growth-promoting bacteria as bioinoculants is a powerful tool to increase crop yield and quality and to improve nitrogen use efficiency (NUE) from fertilizers in plants. This study aimed to bioprospecting a native bacterial consortium ( Bacillus cabrialesii subsp. cabrialesii TE3 T...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/162539 |
| _version_ | 1855517862855180288 |
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| author | Ayala Zepeda, Marisol Valenzuela Ruiz, Valeria Parra Cota, Fannie Isela Chinchilla-Soto, Cristina de la Cruz Torres, Eulogio Ibba, Marí Itria Estrada-Alvarado, María Isabel de los Santos Villalobos, Sergio |
| author_browse | Ayala Zepeda, Marisol Chinchilla-Soto, Cristina Estrada-Alvarado, María Isabel Ibba, Marí Itria Parra Cota, Fannie Isela Valenzuela Ruiz, Valeria de la Cruz Torres, Eulogio de los Santos Villalobos, Sergio |
| author_facet | Ayala Zepeda, Marisol Valenzuela Ruiz, Valeria Parra Cota, Fannie Isela Chinchilla-Soto, Cristina de la Cruz Torres, Eulogio Ibba, Marí Itria Estrada-Alvarado, María Isabel de los Santos Villalobos, Sergio |
| author_sort | Ayala Zepeda, Marisol |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The use of plant growth-promoting bacteria as bioinoculants is a powerful tool to increase crop yield and quality and to improve nitrogen use efficiency (NUE) from fertilizers in plants. This study aimed to bioprospecting a native bacterial consortium ( Bacillus cabrialesii subsp. cabrialesii TE3 T , Priestia megaterium TRQ8, and Bacillus paralicheniformis TRQ65), through bioinformatic analysis, and to quantify the impact of its inoculation on NUE (measured through 15 N-isotopic techniques), grain yield, and grain quality of durum wheat variety CIRNO C2008 grown under three doses of urea (0, 120, and 240 kg N ha -1 ) during two consecutive agricultural cycles in the Yaqui Valley, Mexico. The inoculation of the bacterial consortium (BC) to the wheat crop, at a total N concentration of 123 -225 kg N ha -1 increased crop productivity and maintained grain quality, resulting in a yield increase of 1.1 ton ha -1 (6.0 vs. 7.1 ton ha -1 , 0 kg N ha -1 added, 123 kg N ha -1 in the soil) and of 2.0 ton ha -1 (5.9 vs. 7.9 ton ha -1 , 120 kg N ha -1 added, 104 kg N ha -1 in the soil) compared to the uninoculated controls at the same doses of N. The genomic bioinformatic analysis of the studied strains showed a great number of biofertilization-related genes regarding N and Fe acquisition, P assimilation, CO 2 fixation, Fe, P, and K solubilization, with important roles in agroecosystems, as well as genes related to the production of siderophores and stress response. A positive effect of the BC on NUE at the studied initial N content (123 and 104 kg N ha -1 ) was not observed. Nevertheless, increases of 14 % and 12.5 % on NUE (whole plant) were observed when 120 kg N ha -1 was applied compared to when wheat was fully fertilized (240 kg N ha -1 ). This work represents a link between bioinformatic approaches of a native bacterial inoculant and the quantification of its impact on durum wheat. |
| format | Journal Article |
| id | CGSpace162539 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1625392025-10-26T12:51:34Z Genomic insights of a native bacterial consortium for wheat production sustainability Ayala Zepeda, Marisol Valenzuela Ruiz, Valeria Parra Cota, Fannie Isela Chinchilla-Soto, Cristina de la Cruz Torres, Eulogio Ibba, Marí Itria Estrada-Alvarado, María Isabel de los Santos Villalobos, Sergio nitrogen-use efficiency wheat crop yield quality plant growth-promoting microorganisms The use of plant growth-promoting bacteria as bioinoculants is a powerful tool to increase crop yield and quality and to improve nitrogen use efficiency (NUE) from fertilizers in plants. This study aimed to bioprospecting a native bacterial consortium ( Bacillus cabrialesii subsp. cabrialesii TE3 T , Priestia megaterium TRQ8, and Bacillus paralicheniformis TRQ65), through bioinformatic analysis, and to quantify the impact of its inoculation on NUE (measured through 15 N-isotopic techniques), grain yield, and grain quality of durum wheat variety CIRNO C2008 grown under three doses of urea (0, 120, and 240 kg N ha -1 ) during two consecutive agricultural cycles in the Yaqui Valley, Mexico. The inoculation of the bacterial consortium (BC) to the wheat crop, at a total N concentration of 123 -225 kg N ha -1 increased crop productivity and maintained grain quality, resulting in a yield increase of 1.1 ton ha -1 (6.0 vs. 7.1 ton ha -1 , 0 kg N ha -1 added, 123 kg N ha -1 in the soil) and of 2.0 ton ha -1 (5.9 vs. 7.9 ton ha -1 , 120 kg N ha -1 added, 104 kg N ha -1 in the soil) compared to the uninoculated controls at the same doses of N. The genomic bioinformatic analysis of the studied strains showed a great number of biofertilization-related genes regarding N and Fe acquisition, P assimilation, CO 2 fixation, Fe, P, and K solubilization, with important roles in agroecosystems, as well as genes related to the production of siderophores and stress response. A positive effect of the BC on NUE at the studied initial N content (123 and 104 kg N ha -1 ) was not observed. Nevertheless, increases of 14 % and 12.5 % on NUE (whole plant) were observed when 120 kg N ha -1 was applied compared to when wheat was fully fertilized (240 kg N ha -1 ). This work represents a link between bioinformatic approaches of a native bacterial inoculant and the quantification of its impact on durum wheat. 2024 2024-11-21T15:57:34Z 2024-11-21T15:57:34Z Journal Article https://hdl.handle.net/10568/162539 en Open Access application/pdf Elsevier Ayala Zepeda, M., Valenzuela Ruiz, V., Parra Cota, F. I., Chinchilla-Soto, C., Cruz Torres, E. de la, Ibba, M. I., Estadra Alvarado, M. I., & Santos Villalobos, S. de los (2024). Genomic insights of a native bacterial consortium for wheat production sustainability. Current Research in Microbial Sciences, 6, 100230. https://doi.org/10.1016/j.crmicr.2024.100230 |
| spellingShingle | nitrogen-use efficiency wheat crop yield quality plant growth-promoting microorganisms Ayala Zepeda, Marisol Valenzuela Ruiz, Valeria Parra Cota, Fannie Isela Chinchilla-Soto, Cristina de la Cruz Torres, Eulogio Ibba, Marí Itria Estrada-Alvarado, María Isabel de los Santos Villalobos, Sergio Genomic insights of a native bacterial consortium for wheat production sustainability |
| title | Genomic insights of a native bacterial consortium for wheat production sustainability |
| title_full | Genomic insights of a native bacterial consortium for wheat production sustainability |
| title_fullStr | Genomic insights of a native bacterial consortium for wheat production sustainability |
| title_full_unstemmed | Genomic insights of a native bacterial consortium for wheat production sustainability |
| title_short | Genomic insights of a native bacterial consortium for wheat production sustainability |
| title_sort | genomic insights of a native bacterial consortium for wheat production sustainability |
| topic | nitrogen-use efficiency wheat crop yield quality plant growth-promoting microorganisms |
| url | https://hdl.handle.net/10568/162539 |
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