The wheat sucrose synthase gene TaSus1 is a determinant of grain number per spike
Some haplotypes of the sucrose synthase gene TaSus1 are associated with thousand-grain weight (TGW) in wheat (Triticum aestivum L.). However, no mutations have been identified within the gene to test this association. The effects of TaSus1 on grain number per spike (GNS) also are largely unknown. Ou...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2024
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/162520 |
| _version_ | 1855523588646371328 |
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| author | Liping Shen Lili Zhang Changbin Yin Xiaowan Xu Yangyang Liu Kuocheng Shen He Wu Zhiwen Sun Ke Wang Zhonghu He Xueyong Zhang Chenyang Hao Jian Hou Aoyue Bi Xuebo Zhao Daxing Xu Botao Ye Xuchang Yu Ziying Wang Danni Liu Yuanfeng Hao Fei Lu Zifeng Guo |
| author_browse | Aoyue Bi Botao Ye Changbin Yin Chenyang Hao Danni Liu Daxing Xu Fei Lu He Wu Jian Hou Ke Wang Kuocheng Shen Lili Zhang Liping Shen Xiaowan Xu Xuchang Yu Xuebo Zhao Xueyong Zhang Yangyang Liu Yuanfeng Hao Zhiwen Sun Zhonghu He Zifeng Guo Ziying Wang |
| author_facet | Liping Shen Lili Zhang Changbin Yin Xiaowan Xu Yangyang Liu Kuocheng Shen He Wu Zhiwen Sun Ke Wang Zhonghu He Xueyong Zhang Chenyang Hao Jian Hou Aoyue Bi Xuebo Zhao Daxing Xu Botao Ye Xuchang Yu Ziying Wang Danni Liu Yuanfeng Hao Fei Lu Zifeng Guo |
| author_sort | Liping Shen |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Some haplotypes of the sucrose synthase gene TaSus1 are associated with thousand-grain weight (TGW) in wheat (Triticum aestivum L.). However, no mutations have been identified within the gene to test this association. The effects of TaSus1 on grain number per spike (GNS) also are largely unknown. Our previous genome-wide association study identified TaSus-A1 as a candidate gene controlling fertile spikelet number per spike (FSN). In the present study, we generated two independent mutants for the three TaSus1 homoeologs by CRISPR/Cas9-mediated genome editing. The triple mutants displayed lower FSN, GNS, grain number per spikelet (GNST), and TGW than wild-type plants. In 306 hexaploid wheat accessions, two single-nucleotide polymorphisms in TaSus-A1 contributed differently to GNS. Introgression of the two alleles into a wheat genetic background confirmed their effects. The alleles differed in geographical distribution among the accessions. |
| format | Journal Article |
| id | CGSpace162520 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1625202025-10-26T12:52:02Z The wheat sucrose synthase gene TaSus1 is a determinant of grain number per spike Liping Shen Lili Zhang Changbin Yin Xiaowan Xu Yangyang Liu Kuocheng Shen He Wu Zhiwen Sun Ke Wang Zhonghu He Xueyong Zhang Chenyang Hao Jian Hou Aoyue Bi Xuebo Zhao Daxing Xu Botao Ye Xuchang Yu Ziying Wang Danni Liu Yuanfeng Hao Fei Lu Zifeng Guo grain haplotypes selection wheat Some haplotypes of the sucrose synthase gene TaSus1 are associated with thousand-grain weight (TGW) in wheat (Triticum aestivum L.). However, no mutations have been identified within the gene to test this association. The effects of TaSus1 on grain number per spike (GNS) also are largely unknown. Our previous genome-wide association study identified TaSus-A1 as a candidate gene controlling fertile spikelet number per spike (FSN). In the present study, we generated two independent mutants for the three TaSus1 homoeologs by CRISPR/Cas9-mediated genome editing. The triple mutants displayed lower FSN, GNS, grain number per spikelet (GNST), and TGW than wild-type plants. In 306 hexaploid wheat accessions, two single-nucleotide polymorphisms in TaSus-A1 contributed differently to GNS. Introgression of the two alleles into a wheat genetic background confirmed their effects. The alleles differed in geographical distribution among the accessions. 2024-02 2024-11-21T15:05:39Z 2024-11-21T15:05:39Z Journal Article https://hdl.handle.net/10568/162520 en Open Access application/pdf Elsevier Shen, L., Zhang, L., Yin, C., Xu, X., Liu, Y., Shen, K., Wu, H., Sun, Z., Wang, K., He, Z., Zhang, X., Hao, C., Hou, J., Bi, A., Zhao, X., Xu, D., Ye, B., Yu, X., Wang, Z., . . . Guo, Z. (2024). The wheat sucrose synthase gene TaSus1 is a major determinant of grain number per spike. The Crop Journal, 12(1), 295-300. https://doi.org/10.1016/j.cj.2023.11.007 |
| spellingShingle | grain haplotypes selection wheat Liping Shen Lili Zhang Changbin Yin Xiaowan Xu Yangyang Liu Kuocheng Shen He Wu Zhiwen Sun Ke Wang Zhonghu He Xueyong Zhang Chenyang Hao Jian Hou Aoyue Bi Xuebo Zhao Daxing Xu Botao Ye Xuchang Yu Ziying Wang Danni Liu Yuanfeng Hao Fei Lu Zifeng Guo The wheat sucrose synthase gene TaSus1 is a determinant of grain number per spike |
| title | The wheat sucrose synthase gene TaSus1 is a determinant of grain number per spike |
| title_full | The wheat sucrose synthase gene TaSus1 is a determinant of grain number per spike |
| title_fullStr | The wheat sucrose synthase gene TaSus1 is a determinant of grain number per spike |
| title_full_unstemmed | The wheat sucrose synthase gene TaSus1 is a determinant of grain number per spike |
| title_short | The wheat sucrose synthase gene TaSus1 is a determinant of grain number per spike |
| title_sort | wheat sucrose synthase gene tasus1 is a determinant of grain number per spike |
| topic | grain haplotypes selection wheat |
| url | https://hdl.handle.net/10568/162520 |
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