A mis-splicing early flowering 3 (elf3) allele of lentil is associated with yield enhancement under terminal heat stress
There is a vast scope of area expansion of lentils after harvesting wet rice in South Asia. However, due to the photoperiod effect and terminal heat, the existing short-duration varieties failed to minimize yield loss under late-sown conditions. A mis-splicing causing A/G SNP present in the last nuc...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/160019 |
| _version_ | 1855525830708428800 |
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| author | Roy, Anirban Reddy, M. Hemakumar Sarkar, Moutushi Sagolsem, Diana Murmu, Sumit K. Das, Camellia Roy, Debarati Ganguly, Shamba Nath, Rajib Bhattacharyya, Prabir K. Sarker, Ashutosh Bhattacharyya, Somnath |
| author_browse | Bhattacharyya, Prabir K. Bhattacharyya, Somnath Das, Camellia Ganguly, Shamba Murmu, Sumit K. Nath, Rajib Reddy, M. Hemakumar Roy, Anirban Roy, Debarati Sagolsem, Diana Sarkar, Moutushi Sarker, Ashutosh |
| author_facet | Roy, Anirban Reddy, M. Hemakumar Sarkar, Moutushi Sagolsem, Diana Murmu, Sumit K. Das, Camellia Roy, Debarati Ganguly, Shamba Nath, Rajib Bhattacharyya, Prabir K. Sarker, Ashutosh Bhattacharyya, Somnath |
| author_sort | Roy, Anirban |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | There is a vast scope of area expansion of lentils after harvesting wet rice in South Asia. However, due to the photoperiod effect and terminal heat, the existing short-duration varieties failed to minimize yield loss under late-sown conditions. A mis-splicing causing A/G SNP present in the last nucleotide of exon 3 of early flowering 3 (ELF3) gene (elf3 allele) in a lentil line, L4710, is associated with the photoperiod insensitive flowering and the fast absolute growth rate (AGR). None of the Indian cultivars tested in this study, either early or late, possesses the non-functional elf3 allele. However, the A to G transition in ELF3-exon2 replaces glycine with aspartic acid at the 403rd amino acid in all the Indian varieties tested, compared to the reference sequence of Mediterranean accession, ILL5588. Therefore, targeting A/G SNP of exon 3, a PCR-based codominant marker is developed. The elf3 allele is correlated with the fast AGR and early flowering, but low yield and biomass, in an L4710 × LL56–derived RIL-population, compared to ELF3 carrying alleles when sown on 15th November. However, in a month of delayed sowing (20th December), the same elf3-RILs revealed a higher yield and biomass with slower AGR Moreover, three elf3-carrying lines, grown in delayed condition (20 December) for two consecutive years in three locations, outyielded three popular high-yielding cultivars that carry functional ELF3. Thus, elf3-carrying high-yielding lines could be the breeder’s choice to expand and enhance lentil yield in short-season environments and in vast rice fallows of south Asia, where delayed rice harvest occurs frequently. |
| format | Journal Article |
| id | CGSpace160019 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1600192026-01-13T21:22:49Z A mis-splicing early flowering 3 (elf3) allele of lentil is associated with yield enhancement under terminal heat stress Roy, Anirban Reddy, M. Hemakumar Sarkar, Moutushi Sagolsem, Diana Murmu, Sumit K. Das, Camellia Roy, Debarati Ganguly, Shamba Nath, Rajib Bhattacharyya, Prabir K. Sarker, Ashutosh Bhattacharyya, Somnath heat stress lentils yields lentil absolute growth rate early flowering 3 marker mis-splicing There is a vast scope of area expansion of lentils after harvesting wet rice in South Asia. However, due to the photoperiod effect and terminal heat, the existing short-duration varieties failed to minimize yield loss under late-sown conditions. A mis-splicing causing A/G SNP present in the last nucleotide of exon 3 of early flowering 3 (ELF3) gene (elf3 allele) in a lentil line, L4710, is associated with the photoperiod insensitive flowering and the fast absolute growth rate (AGR). None of the Indian cultivars tested in this study, either early or late, possesses the non-functional elf3 allele. However, the A to G transition in ELF3-exon2 replaces glycine with aspartic acid at the 403rd amino acid in all the Indian varieties tested, compared to the reference sequence of Mediterranean accession, ILL5588. Therefore, targeting A/G SNP of exon 3, a PCR-based codominant marker is developed. The elf3 allele is correlated with the fast AGR and early flowering, but low yield and biomass, in an L4710 × LL56–derived RIL-population, compared to ELF3 carrying alleles when sown on 15th November. However, in a month of delayed sowing (20th December), the same elf3-RILs revealed a higher yield and biomass with slower AGR Moreover, three elf3-carrying lines, grown in delayed condition (20 December) for two consecutive years in three locations, outyielded three popular high-yielding cultivars that carry functional ELF3. Thus, elf3-carrying high-yielding lines could be the breeder’s choice to expand and enhance lentil yield in short-season environments and in vast rice fallows of south Asia, where delayed rice harvest occurs frequently. 2023-02-23 2024-11-20T21:49:24Z 2024-11-20T21:49:24Z Journal Article https://hdl.handle.net/10568/160019 en Limited Access Springer Anirban Roy, M. Hemakumar Reddy, Moutushi Sarkar, Diana Sagolsem, Sumit K. Murmu, Camellia Das, Debarati Roy, Shamba Ganguly, Rajib Nath, Prabir K. Bhattacharyya, Ashutosh Sarker, Somnath Bhattacharyya. (23/2/2023). A mis-splicing early flowering 3 (elf3) allele of lentil is associated with yield enhancement under terminal heat stress. Journal of Applied Genetics, 64, pp. 265-273. |
| spellingShingle | heat stress lentils yields lentil absolute growth rate early flowering 3 marker mis-splicing Roy, Anirban Reddy, M. Hemakumar Sarkar, Moutushi Sagolsem, Diana Murmu, Sumit K. Das, Camellia Roy, Debarati Ganguly, Shamba Nath, Rajib Bhattacharyya, Prabir K. Sarker, Ashutosh Bhattacharyya, Somnath A mis-splicing early flowering 3 (elf3) allele of lentil is associated with yield enhancement under terminal heat stress |
| title | A mis-splicing early flowering 3 (elf3) allele of lentil is associated with yield enhancement under terminal heat stress |
| title_full | A mis-splicing early flowering 3 (elf3) allele of lentil is associated with yield enhancement under terminal heat stress |
| title_fullStr | A mis-splicing early flowering 3 (elf3) allele of lentil is associated with yield enhancement under terminal heat stress |
| title_full_unstemmed | A mis-splicing early flowering 3 (elf3) allele of lentil is associated with yield enhancement under terminal heat stress |
| title_short | A mis-splicing early flowering 3 (elf3) allele of lentil is associated with yield enhancement under terminal heat stress |
| title_sort | mis splicing early flowering 3 elf3 allele of lentil is associated with yield enhancement under terminal heat stress |
| topic | heat stress lentils yields lentil absolute growth rate early flowering 3 marker mis-splicing |
| url | https://hdl.handle.net/10568/160019 |
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