High-density mapping of durable and broad-spectrum stripe rust resistance gene Yr30 in wheat
The emergence of genetically diverse and more aggressive races of Puccinia striiformis f. sp. tritici (Pst) in the past twenty years has resulted in global stripe rust outbreaks and the rapid breakdown of resistance genes. Yr30 is an adult plant resistance (APR) gene with broad-spectrum effectivenes...
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2024
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/159863 |
| _version_ | 1855516300721258496 |
|---|---|
| author | Wang, Xiaoting Xiang, Mingjie Li, Huaizhou Li, Xiaoxiao Mu, Keqing Huang, Shuo Zhang, Yibo Cheng, Xiangrui Yang, Shuqing Yuan, Xunying Singh, Ravi P. Bhavani, Sridhar Zeng, Qingdong Wu, Jianhui Kang, Zhensheng Liu, Shengjie Han, Dejun |
| author_browse | Bhavani, Sridhar Cheng, Xiangrui Han, Dejun Huang, Shuo Kang, Zhensheng Li, Huaizhou Li, Xiaoxiao Liu, Shengjie Mu, Keqing Singh, Ravi P. Wang, Xiaoting Wu, Jianhui Xiang, Mingjie Yang, Shuqing Yuan, Xunying Zeng, Qingdong Zhang, Yibo |
| author_facet | Wang, Xiaoting Xiang, Mingjie Li, Huaizhou Li, Xiaoxiao Mu, Keqing Huang, Shuo Zhang, Yibo Cheng, Xiangrui Yang, Shuqing Yuan, Xunying Singh, Ravi P. Bhavani, Sridhar Zeng, Qingdong Wu, Jianhui Kang, Zhensheng Liu, Shengjie Han, Dejun |
| author_sort | Wang, Xiaoting |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The emergence of genetically diverse and more aggressive races of Puccinia striiformis f. sp. tritici (Pst) in the past twenty years has resulted in global stripe rust outbreaks and the rapid breakdown of resistance genes. Yr30 is an adult plant resistance (APR) gene with broad-spectrum effectiveness and its durability. Here, we fine-mapped the YR30 locus to a 0.52-cM interval using 1629 individuals derived from residual heterozygous F-5:6 plants in a Yaco"S"/Mingxian169 recombinant inbred line population. This interval corresponded to a 610-kb region in the International Wheat Genome Sequencing Consortium (IWGSC) RefSeq version 2.1 on chromosome arm 3BS harboring 30 high-confidence genes. Five genes were identified as candidate genes based on functional annotation, expression analysis by RNA-seq and sequence polymorphisms between cultivars with and without Yr30 based on resequencing. Haplotype analysis of the target region identified six haplotypes (YR30_h1-YR30_h6) in a panel of 1215 wheat accessions based on the 660K feature genotyping array. Lines with YR30_h6 displayed more resistance to stripe rust than the other five haplotypes. Near-isogenic lines (NILs) with Yr30 showed a 32.94% higher grain yield than susceptible counterparts when grown in a stripe rust nursery, whereas there was no difference in grain yield under rust-free conditions. These results lay a foundation for map-based cloning Yr30. |
| format | Journal Article |
| id | CGSpace159863 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1598632025-12-08T10:11:39Z High-density mapping of durable and broad-spectrum stripe rust resistance gene Yr30 in wheat Wang, Xiaoting Xiang, Mingjie Li, Huaizhou Li, Xiaoxiao Mu, Keqing Huang, Shuo Zhang, Yibo Cheng, Xiangrui Yang, Shuqing Yuan, Xunying Singh, Ravi P. Bhavani, Sridhar Zeng, Qingdong Wu, Jianhui Kang, Zhensheng Liu, Shengjie Han, Dejun stripe rust resistance genes wheat genetic markers chromosome mapping The emergence of genetically diverse and more aggressive races of Puccinia striiformis f. sp. tritici (Pst) in the past twenty years has resulted in global stripe rust outbreaks and the rapid breakdown of resistance genes. Yr30 is an adult plant resistance (APR) gene with broad-spectrum effectiveness and its durability. Here, we fine-mapped the YR30 locus to a 0.52-cM interval using 1629 individuals derived from residual heterozygous F-5:6 plants in a Yaco"S"/Mingxian169 recombinant inbred line population. This interval corresponded to a 610-kb region in the International Wheat Genome Sequencing Consortium (IWGSC) RefSeq version 2.1 on chromosome arm 3BS harboring 30 high-confidence genes. Five genes were identified as candidate genes based on functional annotation, expression analysis by RNA-seq and sequence polymorphisms between cultivars with and without Yr30 based on resequencing. Haplotype analysis of the target region identified six haplotypes (YR30_h1-YR30_h6) in a panel of 1215 wheat accessions based on the 660K feature genotyping array. Lines with YR30_h6 displayed more resistance to stripe rust than the other five haplotypes. Near-isogenic lines (NILs) with Yr30 showed a 32.94% higher grain yield than susceptible counterparts when grown in a stripe rust nursery, whereas there was no difference in grain yield under rust-free conditions. These results lay a foundation for map-based cloning Yr30. 2024-07 2024-11-15T20:16:20Z 2024-11-15T20:16:20Z Journal Article https://hdl.handle.net/10568/159863 en Limited Access Springer Wang, X., Xiang, M., Li, H., Li, X., Mu, K., Huang, S., Zhang, Y., Cheng, X., Yang, S., Yuan, X., Singh, R. P., Bhavani, S., Zeng, Q., Wu, J., Kang, Z., Liu, S., & Han, D. (2024). High-density mapping of durable and broad-spectrum stripe rust resistance gene Yr30 in wheat. Theoretical And Applied Genetics, 137(7): 152. https://doi.org/10.1007/s00122-024-04654-5 |
| spellingShingle | stripe rust resistance genes wheat genetic markers chromosome mapping Wang, Xiaoting Xiang, Mingjie Li, Huaizhou Li, Xiaoxiao Mu, Keqing Huang, Shuo Zhang, Yibo Cheng, Xiangrui Yang, Shuqing Yuan, Xunying Singh, Ravi P. Bhavani, Sridhar Zeng, Qingdong Wu, Jianhui Kang, Zhensheng Liu, Shengjie Han, Dejun High-density mapping of durable and broad-spectrum stripe rust resistance gene Yr30 in wheat |
| title | High-density mapping of durable and broad-spectrum stripe rust resistance gene Yr30 in wheat |
| title_full | High-density mapping of durable and broad-spectrum stripe rust resistance gene Yr30 in wheat |
| title_fullStr | High-density mapping of durable and broad-spectrum stripe rust resistance gene Yr30 in wheat |
| title_full_unstemmed | High-density mapping of durable and broad-spectrum stripe rust resistance gene Yr30 in wheat |
| title_short | High-density mapping of durable and broad-spectrum stripe rust resistance gene Yr30 in wheat |
| title_sort | high density mapping of durable and broad spectrum stripe rust resistance gene yr30 in wheat |
| topic | stripe rust resistance genes wheat genetic markers chromosome mapping |
| url | https://hdl.handle.net/10568/159863 |
| work_keys_str_mv | AT wangxiaoting highdensitymappingofdurableandbroadspectrumstriperustresistancegeneyr30inwheat AT xiangmingjie highdensitymappingofdurableandbroadspectrumstriperustresistancegeneyr30inwheat AT lihuaizhou highdensitymappingofdurableandbroadspectrumstriperustresistancegeneyr30inwheat AT lixiaoxiao highdensitymappingofdurableandbroadspectrumstriperustresistancegeneyr30inwheat AT mukeqing highdensitymappingofdurableandbroadspectrumstriperustresistancegeneyr30inwheat AT huangshuo highdensitymappingofdurableandbroadspectrumstriperustresistancegeneyr30inwheat AT zhangyibo highdensitymappingofdurableandbroadspectrumstriperustresistancegeneyr30inwheat AT chengxiangrui highdensitymappingofdurableandbroadspectrumstriperustresistancegeneyr30inwheat AT yangshuqing highdensitymappingofdurableandbroadspectrumstriperustresistancegeneyr30inwheat AT yuanxunying highdensitymappingofdurableandbroadspectrumstriperustresistancegeneyr30inwheat AT singhravip highdensitymappingofdurableandbroadspectrumstriperustresistancegeneyr30inwheat AT bhavanisridhar highdensitymappingofdurableandbroadspectrumstriperustresistancegeneyr30inwheat AT zengqingdong highdensitymappingofdurableandbroadspectrumstriperustresistancegeneyr30inwheat AT wujianhui highdensitymappingofdurableandbroadspectrumstriperustresistancegeneyr30inwheat AT kangzhensheng highdensitymappingofdurableandbroadspectrumstriperustresistancegeneyr30inwheat AT liushengjie highdensitymappingofdurableandbroadspectrumstriperustresistancegeneyr30inwheat AT handejun highdensitymappingofdurableandbroadspectrumstriperustresistancegeneyr30inwheat |