Genetic control of root architectural traits under drought stress in spring barley (Hordeum vulgare L.)
Root architectural traits play pivotal roles in plant adaptation to drought stress, and hence they are considered promising targets in breeding programs. Here, we phenotyped eight root architecture traits in response to well-watered and drought stress conditions in 200 spring barley (Hordeum vulgare...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Crop Science Society of America
2024
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/159772 |
| _version_ | 1855537081395183616 |
|---|---|
| author | Siddiqui, Nurealam Jahiu, Melisa Kamruzzaman, Mohammad Sanchez-Garcia, Miguel Mason, Annaliese S. Leon, Jens Ballvora, Agim |
| author_browse | Ballvora, Agim Jahiu, Melisa Kamruzzaman, Mohammad Leon, Jens Mason, Annaliese S. Sanchez-Garcia, Miguel Siddiqui, Nurealam |
| author_facet | Siddiqui, Nurealam Jahiu, Melisa Kamruzzaman, Mohammad Sanchez-Garcia, Miguel Mason, Annaliese S. Leon, Jens Ballvora, Agim |
| author_sort | Siddiqui, Nurealam |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Root architectural traits play pivotal roles in plant adaptation to drought stress, and hence they are considered promising targets in breeding programs. Here, we phenotyped eight root architecture traits in response to well-watered and drought stress conditions in 200 spring barley (Hordeum vulgare L.) inbred lines over two consecutive field seasons. Root architecture traits were less developed under drought in both seasons when compared with control treatments. Genetic variation in root architectural traits was dissected employing a genome-wide association study (GWAS) coupled with linkage disequilibrium mapping. GWAS uncovered a total of 186 significant single nucleotide polymorphism-trait associations for eight root traits under control, drought, and drought-related indices. Of these, a few loci for root traits were detected on chromosomes 3 and 5, which co-located with QTL identified in previous studies. Interestingly, 13 loci showed simultaneou associations with multiple root traits under drought and drought-related indices. These loci harbored candidate genes, which included a wide range of drought-responsive components such as transcription factors, binding proteins, protein kinases, nutrient and ion transporters, and stress signaling factors. For instance, two candidate genes, HORVU7Hr3G0713160 and HORVU6H r3G0626550, are orthologous to AtACX3 and AtVAMPs, which have reported functions in root length-mediated drought tolerance and as a key protein in abiotic stress tolerance, respectively. Interestingly, one of these loci underlying a high-confidence candidate gene NEW ENHANCER OF ROOT DWARFISM1 (NERD1) showed involvement with root development. An allelic variation of this locus in non-coding region was significantly associated with increased root length under drought. Collectively, these results offer promising multi-trait affecting loci and candidate genes underlying root phenotypic responses to drought stress, which may provide valuable resources for genetic improvement of drought tolerance in barley. |
| format | Journal Article |
| id | CGSpace159772 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Crop Science Society of America |
| publisherStr | Crop Science Society of America |
| record_format | dspace |
| spelling | CGSpace1597722026-01-14T02:14:42Z Genetic control of root architectural traits under drought stress in spring barley (Hordeum vulgare L.) Siddiqui, Nurealam Jahiu, Melisa Kamruzzaman, Mohammad Sanchez-Garcia, Miguel Mason, Annaliese S. Leon, Jens Ballvora, Agim spring barley phenotyping linkage disequilibrium mapping single nucleotide polymorphisms spring barley genome-wide association study root architectural traits drought stress adaptation Root architectural traits play pivotal roles in plant adaptation to drought stress, and hence they are considered promising targets in breeding programs. Here, we phenotyped eight root architecture traits in response to well-watered and drought stress conditions in 200 spring barley (Hordeum vulgare L.) inbred lines over two consecutive field seasons. Root architecture traits were less developed under drought in both seasons when compared with control treatments. Genetic variation in root architectural traits was dissected employing a genome-wide association study (GWAS) coupled with linkage disequilibrium mapping. GWAS uncovered a total of 186 significant single nucleotide polymorphism-trait associations for eight root traits under control, drought, and drought-related indices. Of these, a few loci for root traits were detected on chromosomes 3 and 5, which co-located with QTL identified in previous studies. Interestingly, 13 loci showed simultaneou associations with multiple root traits under drought and drought-related indices. These loci harbored candidate genes, which included a wide range of drought-responsive components such as transcription factors, binding proteins, protein kinases, nutrient and ion transporters, and stress signaling factors. For instance, two candidate genes, HORVU7Hr3G0713160 and HORVU6H r3G0626550, are orthologous to AtACX3 and AtVAMPs, which have reported functions in root length-mediated drought tolerance and as a key protein in abiotic stress tolerance, respectively. Interestingly, one of these loci underlying a high-confidence candidate gene NEW ENHANCER OF ROOT DWARFISM1 (NERD1) showed involvement with root development. An allelic variation of this locus in non-coding region was significantly associated with increased root length under drought. Collectively, these results offer promising multi-trait affecting loci and candidate genes underlying root phenotypic responses to drought stress, which may provide valuable resources for genetic improvement of drought tolerance in barley. 2024-06-01 2024-11-14T17:25:47Z 2024-11-14T17:25:47Z Journal Article https://hdl.handle.net/10568/159772 en Open Access application/pdf Crop Science Society of America Nurealam Siddiqui, Melisa Jahiu, Mohammad Kamruzzaman, Miguel Sanchez-Garcia, Annaliese S. Mason, Jens Leon, Agim Ballvora. (1/6/2024). Genetic control of root architectural traits under drought stress in spring barley (Hordeum vulgare L. ). The Plant Genome, 17 (2). |
| spellingShingle | spring barley phenotyping linkage disequilibrium mapping single nucleotide polymorphisms spring barley genome-wide association study root architectural traits drought stress adaptation Siddiqui, Nurealam Jahiu, Melisa Kamruzzaman, Mohammad Sanchez-Garcia, Miguel Mason, Annaliese S. Leon, Jens Ballvora, Agim Genetic control of root architectural traits under drought stress in spring barley (Hordeum vulgare L.) |
| title | Genetic control of root architectural traits under drought stress in spring barley (Hordeum vulgare L.) |
| title_full | Genetic control of root architectural traits under drought stress in spring barley (Hordeum vulgare L.) |
| title_fullStr | Genetic control of root architectural traits under drought stress in spring barley (Hordeum vulgare L.) |
| title_full_unstemmed | Genetic control of root architectural traits under drought stress in spring barley (Hordeum vulgare L.) |
| title_short | Genetic control of root architectural traits under drought stress in spring barley (Hordeum vulgare L.) |
| title_sort | genetic control of root architectural traits under drought stress in spring barley hordeum vulgare l |
| topic | spring barley phenotyping linkage disequilibrium mapping single nucleotide polymorphisms spring barley genome-wide association study root architectural traits drought stress adaptation |
| url | https://hdl.handle.net/10568/159772 |
| work_keys_str_mv | AT siddiquinurealam geneticcontrolofrootarchitecturaltraitsunderdroughtstressinspringbarleyhordeumvulgarel AT jahiumelisa geneticcontrolofrootarchitecturaltraitsunderdroughtstressinspringbarleyhordeumvulgarel AT kamruzzamanmohammad geneticcontrolofrootarchitecturaltraitsunderdroughtstressinspringbarleyhordeumvulgarel AT sanchezgarciamiguel geneticcontrolofrootarchitecturaltraitsunderdroughtstressinspringbarleyhordeumvulgarel AT masonannalieses geneticcontrolofrootarchitecturaltraitsunderdroughtstressinspringbarleyhordeumvulgarel AT leonjens geneticcontrolofrootarchitecturaltraitsunderdroughtstressinspringbarleyhordeumvulgarel AT ballvoraagim geneticcontrolofrootarchitecturaltraitsunderdroughtstressinspringbarleyhordeumvulgarel |