Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Alfalfa mosaic virus (AMV) in legume crops applied by ICARDA’s Seed Health and Virology Laboratory
Alfalfa mosaic virus (AMV, genus Alfamovirus, family Bromoviridae) is a significant plant pathogen affecting a wide range of legume crops across various regions of the world including West Asia and North Africa (WANA) countries. AMV is transmitted by aphids in a non-persistent manner and via seeds o...
| Autores principales: | , |
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| Formato: | Tool |
| Lenguaje: | Inglés |
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International Center for Agricultural Research in the Dry Areas
2024
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| Acceso en línea: | https://hdl.handle.net/10568/159753 |
| _version_ | 1855514085623332864 |
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| author | Kumari, Safaa Moukahel, Abdulrahman |
| author_browse | Kumari, Safaa Moukahel, Abdulrahman |
| author_facet | Kumari, Safaa Moukahel, Abdulrahman |
| author_sort | Kumari, Safaa |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Alfalfa mosaic virus (AMV, genus Alfamovirus, family Bromoviridae) is a significant plant pathogen affecting a wide range of legume crops across various regions of the world including West Asia and North Africa (WANA) countries. AMV is transmitted by aphids in a non-persistent manner and via seeds of several hosts (including faba bean, chickpea, and lentil). Seed transmission makes managing AMV more challenging, as infected seeds can introduce the virus into new areas and lead to infections in the next growing season, even before aphid vectors are active. Accordingly, seed testing and the use of certified seeds are key strategies to mitigate the spread of AMV as a seed-borne virus. Early detection and accurate diagnosis of viral diseases is critical for the application of appropriate control measures. However, the accuracy and reliability of molecular methods is a fact beyond dispute. This report describes a validated molecular tool for AMV detection and characterization applied by ICARDA’s Seed Health and Virology Laboratory. |
| format | Tool |
| id | CGSpace159753 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | International Center for Agricultural Research in the Dry Areas |
| publisherStr | International Center for Agricultural Research in the Dry Areas |
| record_format | dspace |
| spelling | CGSpace1597532026-01-15T02:00:54Z Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Alfalfa mosaic virus (AMV) in legume crops applied by ICARDA’s Seed Health and Virology Laboratory Kumari, Safaa Moukahel, Abdulrahman faba bean lentil chickpea alfalfa legume common bean grass pea (lathyrus sativus) medics legume viruses rt-pcr seedborne diseases ic-rt-pcr legume field pea (pisum sativum) Alfalfa mosaic virus (AMV, genus Alfamovirus, family Bromoviridae) is a significant plant pathogen affecting a wide range of legume crops across various regions of the world including West Asia and North Africa (WANA) countries. AMV is transmitted by aphids in a non-persistent manner and via seeds of several hosts (including faba bean, chickpea, and lentil). Seed transmission makes managing AMV more challenging, as infected seeds can introduce the virus into new areas and lead to infections in the next growing season, even before aphid vectors are active. Accordingly, seed testing and the use of certified seeds are key strategies to mitigate the spread of AMV as a seed-borne virus. Early detection and accurate diagnosis of viral diseases is critical for the application of appropriate control measures. However, the accuracy and reliability of molecular methods is a fact beyond dispute. This report describes a validated molecular tool for AMV detection and characterization applied by ICARDA’s Seed Health and Virology Laboratory. 2024-11-12 2024-11-14T15:21:34Z 2024-11-14T15:21:34Z Tool https://hdl.handle.net/10568/159753 en Open Access application/pdf International Center for Agricultural Research in the Dry Areas Safaa G. Kumari and Abdelrahman Moukahel. 2024. Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Alfalfa mosaic virus (AMV) in legume crops applied by ICARDA’s Seed Health and Virology Laboratory. International Center for Agricultural Research in the Dry Areas (ICARDA), Beirut, Lebanon. |
| spellingShingle | faba bean lentil chickpea alfalfa legume common bean grass pea (lathyrus sativus) medics legume viruses rt-pcr seedborne diseases ic-rt-pcr legume field pea (pisum sativum) Kumari, Safaa Moukahel, Abdulrahman Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Alfalfa mosaic virus (AMV) in legume crops applied by ICARDA’s Seed Health and Virology Laboratory |
| title | Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Alfalfa mosaic virus (AMV) in legume crops applied by ICARDA’s Seed Health and Virology Laboratory |
| title_full | Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Alfalfa mosaic virus (AMV) in legume crops applied by ICARDA’s Seed Health and Virology Laboratory |
| title_fullStr | Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Alfalfa mosaic virus (AMV) in legume crops applied by ICARDA’s Seed Health and Virology Laboratory |
| title_full_unstemmed | Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Alfalfa mosaic virus (AMV) in legume crops applied by ICARDA’s Seed Health and Virology Laboratory |
| title_short | Reverse transcription-polymerase chain reaction (RT-PCR) assay for detection and characterization of Alfalfa mosaic virus (AMV) in legume crops applied by ICARDA’s Seed Health and Virology Laboratory |
| title_sort | reverse transcription polymerase chain reaction rt pcr assay for detection and characterization of alfalfa mosaic virus amv in legume crops applied by icarda s seed health and virology laboratory |
| topic | faba bean lentil chickpea alfalfa legume common bean grass pea (lathyrus sativus) medics legume viruses rt-pcr seedborne diseases ic-rt-pcr legume field pea (pisum sativum) |
| url | https://hdl.handle.net/10568/159753 |
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