Editing cassava sweet genes for resistance to Xanthomonas. Reporting period: Second Semester 2024
Cassava (Manihot esculenta Crantz) is considered the third most important crop globally. The mitigation of diseases like cassava bacterial blight (CBB), caused by the phytopathogenic bacterium Xanthomonas phaseoli pv. manihotis (Xpm) is key for the success of the crop. CBB leads to significant yiel...
| Autores principales: | , , , , |
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| Formato: | Informe técnico |
| Lenguaje: | Inglés |
| Publicado: |
2024
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/159487 |
| _version_ | 1855517960333950976 |
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| author | Sanchez, Francisco J. Zárate, Carlos A. Szurek, Boris Díaz, Paula A. Chavarriaga, Paul |
| author_browse | Chavarriaga, Paul Díaz, Paula A. Sanchez, Francisco J. Szurek, Boris Zárate, Carlos A. |
| author_facet | Sanchez, Francisco J. Zárate, Carlos A. Szurek, Boris Díaz, Paula A. Chavarriaga, Paul |
| author_sort | Sanchez, Francisco J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava (Manihot esculenta Crantz) is considered the third most important crop globally. The mitigation of diseases like cassava bacterial blight (CBB), caused by the phytopathogenic bacterium Xanthomonas phaseoli pv. manihotis (Xpm) is key for the success of the crop. CBB leads to significant yield losses, ranging from 12% to 100%. One of the infection mechanisms employed by Xpm involves TALE-type proteins, which facilitate bacterial proliferation and the onset of disease symptoms. It has been demonstrated that virulence mechanisms activate certain gene families, including the SWEET gene family, which encodes sugar transporters (such as glucose and sucrose) that provide a carbon source for the bacteria to grow a cause infection.
The aim of our research is to assess the resistance of cassava lines edited in the MeSweet 10a and 10e genes using CRISPR/Cas9 to confer resistance to CBB in the model variety 60444, susceptible to CBB. The goal is to evaluate the impact of editions in the promoter or coding regions for resistance to Xpm infection. Lines exhibiting the most promising mutations (INDELS) in targeted regions will be identified through molecular assays, whichthen will be established under in vitro and greenhouse conditions to be infected with Xpm strains and evaluatedisease resistance. |
| format | Informe técnico |
| id | CGSpace159487 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| record_format | dspace |
| spelling | CGSpace1594872025-12-08T09:54:28Z Editing cassava sweet genes for resistance to Xanthomonas. Reporting period: Second Semester 2024 Sanchez, Francisco J. Zárate, Carlos A. Szurek, Boris Díaz, Paula A. Chavarriaga, Paul cassava gene editing crispr Cassava (Manihot esculenta Crantz) is considered the third most important crop globally. The mitigation of diseases like cassava bacterial blight (CBB), caused by the phytopathogenic bacterium Xanthomonas phaseoli pv. manihotis (Xpm) is key for the success of the crop. CBB leads to significant yield losses, ranging from 12% to 100%. One of the infection mechanisms employed by Xpm involves TALE-type proteins, which facilitate bacterial proliferation and the onset of disease symptoms. It has been demonstrated that virulence mechanisms activate certain gene families, including the SWEET gene family, which encodes sugar transporters (such as glucose and sucrose) that provide a carbon source for the bacteria to grow a cause infection. The aim of our research is to assess the resistance of cassava lines edited in the MeSweet 10a and 10e genes using CRISPR/Cas9 to confer resistance to CBB in the model variety 60444, susceptible to CBB. The goal is to evaluate the impact of editions in the promoter or coding regions for resistance to Xpm infection. Lines exhibiting the most promising mutations (INDELS) in targeted regions will be identified through molecular assays, whichthen will be established under in vitro and greenhouse conditions to be infected with Xpm strains and evaluatedisease resistance. 2024-10 2024-11-11T09:31:13Z 2024-11-11T09:31:13Z Report https://hdl.handle.net/10568/159487 en Open Access application/pdf Sanchez, F.J.; Zárate, C.A.; Szurek, B.; Díaz, P.A.; Chavarriaga, P. (2024) Editing cassava sweet genes for resistance to Xanthomonas. Reporting period: Second Semester 2024. 12 p. |
| spellingShingle | cassava gene editing crispr Sanchez, Francisco J. Zárate, Carlos A. Szurek, Boris Díaz, Paula A. Chavarriaga, Paul Editing cassava sweet genes for resistance to Xanthomonas. Reporting period: Second Semester 2024 |
| title | Editing cassava sweet genes for resistance to Xanthomonas. Reporting period: Second Semester 2024 |
| title_full | Editing cassava sweet genes for resistance to Xanthomonas. Reporting period: Second Semester 2024 |
| title_fullStr | Editing cassava sweet genes for resistance to Xanthomonas. Reporting period: Second Semester 2024 |
| title_full_unstemmed | Editing cassava sweet genes for resistance to Xanthomonas. Reporting period: Second Semester 2024 |
| title_short | Editing cassava sweet genes for resistance to Xanthomonas. Reporting period: Second Semester 2024 |
| title_sort | editing cassava sweet genes for resistance to xanthomonas reporting period second semester 2024 |
| topic | cassava gene editing crispr |
| url | https://hdl.handle.net/10568/159487 |
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