Individual-breed assignments in caprine populations using microsatellite DNA analysis
Genetic variability of 18 microsatellites in five Chinese goat breeds was characterized and then individual-breed assignments were implemented on the basis of microsatellite multi-genotypes. Although the low Gst value of 5.7% occurred among these breeds, the accumulated non-parental exclusion probab...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2008
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/1580 |
| _version_ | 1855520287916818432 |
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| author | Fan, B. Chen, S.L. Mburu, D.N. Hanotte, Olivier H. Chen, Q.K. Zhao, S.K. Li, K. Han Jianlin |
| author_browse | Chen, Q.K. Chen, S.L. Fan, B. Han Jianlin Hanotte, Olivier H. Li, K. Mburu, D.N. Zhao, S.K. |
| author_facet | Fan, B. Chen, S.L. Mburu, D.N. Hanotte, Olivier H. Chen, Q.K. Zhao, S.K. Li, K. Han Jianlin |
| author_sort | Fan, B. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Genetic variability of 18 microsatellites in five Chinese goat breeds was characterized and then individual-breed assignments were implemented on the basis of microsatellite multi-genotypes. Although the low Gst value of 5.7% occurred among these breeds, the accumulated non-parental exclusion probabilities of 99.99% and the correct assignment percentage of 82% demonstrated that microsatellites were feasible for paternity testing and individual-breed assignments in caprine populations. All of loci showed high polymorphism except that MAF35 had only two alleles, and INRA0132 had potential null allele in these breeds. The non-parental exclusion probability per locus varied from 0.081 to 0.534 under case I for paternity testing and ranged from 0.161 to 0.699 under case II, respectively. Both of Excl 1 and Excl 2 were significantly correlated to the genetic diversity measures including genetic heterozygosity, polymorphism information content (PIC) and gene differential coefficients, and that suggested genetic polymorphism of marker provide enough information for microsatellite selection. In addition, the Bayesian method was proven to have superiority of the correct assignment percentage in comparison to gene frequency method and genetic distance methods, with the assumptions of locus-population Hardy–Weinberg Equilibrium and no linkage between loci. The correct assignment percentage were always higher in YC and Boer than other three goat breeds, implying assignment efficiency was positively relative to populations with greater uniformity and low population genetic variability. The correct assignment percentage rose with the increase of number of loci used, and it could reach 71.5% when nine microsatellites with the highest genetic polymorphism were combined together. |
| format | Journal Article |
| id | CGSpace1580 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2008 |
| publishDateRange | 2008 |
| publishDateSort | 2008 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace15802024-05-01T08:16:14Z Individual-breed assignments in caprine populations using microsatellite DNA analysis Fan, B. Chen, S.L. Mburu, D.N. Hanotte, Olivier H. Chen, Q.K. Zhao, S.K. Li, K. Han Jianlin animal breeding genetics Genetic variability of 18 microsatellites in five Chinese goat breeds was characterized and then individual-breed assignments were implemented on the basis of microsatellite multi-genotypes. Although the low Gst value of 5.7% occurred among these breeds, the accumulated non-parental exclusion probabilities of 99.99% and the correct assignment percentage of 82% demonstrated that microsatellites were feasible for paternity testing and individual-breed assignments in caprine populations. All of loci showed high polymorphism except that MAF35 had only two alleles, and INRA0132 had potential null allele in these breeds. The non-parental exclusion probability per locus varied from 0.081 to 0.534 under case I for paternity testing and ranged from 0.161 to 0.699 under case II, respectively. Both of Excl 1 and Excl 2 were significantly correlated to the genetic diversity measures including genetic heterozygosity, polymorphism information content (PIC) and gene differential coefficients, and that suggested genetic polymorphism of marker provide enough information for microsatellite selection. In addition, the Bayesian method was proven to have superiority of the correct assignment percentage in comparison to gene frequency method and genetic distance methods, with the assumptions of locus-population Hardy–Weinberg Equilibrium and no linkage between loci. The correct assignment percentage were always higher in YC and Boer than other three goat breeds, implying assignment efficiency was positively relative to populations with greater uniformity and low population genetic variability. The correct assignment percentage rose with the increase of number of loci used, and it could reach 71.5% when nine microsatellites with the highest genetic polymorphism were combined together. 2008-03 2010-05-19T19:55:07Z 2010-05-19T19:55:07Z Journal Article https://hdl.handle.net/10568/1580 en Limited Access Elsevier Fan, B.; Jianlin, H.; Chen, S-L.; Mburu, D.N.; Hanotte, O.; Chen, Qi-K.; Zhao, Shu-K.; Li, K. 2008. Individual-breed assignments in caprine populations using microsatellite DNA analysis. Small Ruminant Research 75(2-3):154-161. |
| spellingShingle | animal breeding genetics Fan, B. Chen, S.L. Mburu, D.N. Hanotte, Olivier H. Chen, Q.K. Zhao, S.K. Li, K. Han Jianlin Individual-breed assignments in caprine populations using microsatellite DNA analysis |
| title | Individual-breed assignments in caprine populations using microsatellite DNA analysis |
| title_full | Individual-breed assignments in caprine populations using microsatellite DNA analysis |
| title_fullStr | Individual-breed assignments in caprine populations using microsatellite DNA analysis |
| title_full_unstemmed | Individual-breed assignments in caprine populations using microsatellite DNA analysis |
| title_short | Individual-breed assignments in caprine populations using microsatellite DNA analysis |
| title_sort | individual breed assignments in caprine populations using microsatellite dna analysis |
| topic | animal breeding genetics |
| url | https://hdl.handle.net/10568/1580 |
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