HarvestPlus handbook for carotenoid analysis

Carotenoid analysis is inherently difficult and error prone. Despite substantial improvements and refinements in the methods for analyzing these fascinating but complicated compounds, discrepancies in analytical data can still be perceived in the literature. Analysts must be well informed about the...

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Detalles Bibliográficos
Autores principales: Rodriguez-Amaya, Delia B., Kimura, Mihoko
Formato: Libro
Lenguaje:Inglés
Publicado: International Food Policy Research Institute 2004
Materias:
Acceso en línea:https://hdl.handle.net/10568/157384
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author Rodriguez-Amaya, Delia B.
Kimura, Mihoko
author_browse Kimura, Mihoko
Rodriguez-Amaya, Delia B.
author_facet Rodriguez-Amaya, Delia B.
Kimura, Mihoko
author_sort Rodriguez-Amaya, Delia B.
collection Repository of Agricultural Research Outputs (CGSpace)
description Carotenoid analysis is inherently difficult and error prone. Despite substantial improvements and refinements in the methods for analyzing these fascinating but complicated compounds, discrepancies in analytical data can still be perceived in the literature. Analysts must be well informed about the nature and properties of carotenoids and the problems associated with their identification and quantification. Carotenoid analysis consists of several steps that can be carried out efficiently in various ways. Thus, in this handbook several procedures are given for some steps to enable the analyst to choose the procedure that best suits his/her laboratory conditions and experience. These procedures have been put together and evaluated for HarvestPlus crops in our laboratory, but we recognize that other procedures may also produce reliable results. It is important that analysts test the methods in their own laboratory prior to performing the actual analyses. Knowledge of the purpose and possible sources of error in each step will assist the analyst in appraising the performances of the methods. Indeed, the key element in the accurate determination of carotenoids is undoubtedly the analyst. Even a well-validated method may perform badly in the hands of an analyst who does not seek the appropriate information and does not pay attention to the many important details that are necessary to ensure the accuracy of the analytical results.
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spelling CGSpace1573842025-01-10T06:35:53Z HarvestPlus handbook for carotenoid analysis Rodriguez-Amaya, Delia B. Kimura, Mihoko sweet potatoes cassava carotenoids retinol plant breeding xanthophylls proximate composition maize chromatography analytical methods processed foods health Carotenoid analysis is inherently difficult and error prone. Despite substantial improvements and refinements in the methods for analyzing these fascinating but complicated compounds, discrepancies in analytical data can still be perceived in the literature. Analysts must be well informed about the nature and properties of carotenoids and the problems associated with their identification and quantification. Carotenoid analysis consists of several steps that can be carried out efficiently in various ways. Thus, in this handbook several procedures are given for some steps to enable the analyst to choose the procedure that best suits his/her laboratory conditions and experience. These procedures have been put together and evaluated for HarvestPlus crops in our laboratory, but we recognize that other procedures may also produce reliable results. It is important that analysts test the methods in their own laboratory prior to performing the actual analyses. Knowledge of the purpose and possible sources of error in each step will assist the analyst in appraising the performances of the methods. Indeed, the key element in the accurate determination of carotenoids is undoubtedly the analyst. Even a well-validated method may perform badly in the hands of an analyst who does not seek the appropriate information and does not pay attention to the many important details that are necessary to ensure the accuracy of the analytical results. 2004 2024-10-24T12:49:23Z 2024-10-24T12:49:23Z Book https://hdl.handle.net/10568/157384 en Open Access application/pdf International Food Policy Research Institute International Center for Tropical Agriculture Rodriguez-Amaya, Delia B.; Kimura, Mihoko. 2004. HarvestPlus handbook for carotenoid analysis. HarvestPlus Technical Monographs. 2. https://hdl.handle.net/10568/157384
spellingShingle sweet potatoes
cassava
carotenoids
retinol
plant breeding
xanthophylls
proximate composition
maize
chromatography
analytical methods
processed foods
health
Rodriguez-Amaya, Delia B.
Kimura, Mihoko
HarvestPlus handbook for carotenoid analysis
title HarvestPlus handbook for carotenoid analysis
title_full HarvestPlus handbook for carotenoid analysis
title_fullStr HarvestPlus handbook for carotenoid analysis
title_full_unstemmed HarvestPlus handbook for carotenoid analysis
title_short HarvestPlus handbook for carotenoid analysis
title_sort harvestplus handbook for carotenoid analysis
topic sweet potatoes
cassava
carotenoids
retinol
plant breeding
xanthophylls
proximate composition
maize
chromatography
analytical methods
processed foods
health
url https://hdl.handle.net/10568/157384
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