Assessment of in vitro interferon-gamma responses from peripheral blood mononuclear cells of cattle infected with Mycoplasma mycoides ssp. mycoides small colony type
Contagious bovine pleuropneumonia (CBPP) is a lung disease caused by the bacterial pathogen Mycoplasma mycoides ssp. mycoides small colony type (MmmSC). It has been spreading due to a number of factors including poor vaccine efficacy and poor sensitivity of current diagnostic tests. The purpose of t...
| Autores principales: | , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Elsevier
2008
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| Acceso en línea: | https://hdl.handle.net/10568/1573 |
| _version_ | 1855530707355435008 |
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| author | Jores, Joerg Nkando, I. Sterner-Kock, Anja Haider, W. Poole, Elizabeth J. Unger, H. Muriuki, C. Wesonga, H.O. Taracha, E.L.N. |
| author_browse | Haider, W. Jores, Joerg Muriuki, C. Nkando, I. Poole, Elizabeth J. Sterner-Kock, Anja Taracha, E.L.N. Unger, H. Wesonga, H.O. |
| author_facet | Jores, Joerg Nkando, I. Sterner-Kock, Anja Haider, W. Poole, Elizabeth J. Unger, H. Muriuki, C. Wesonga, H.O. Taracha, E.L.N. |
| author_sort | Jores, Joerg |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Contagious bovine pleuropneumonia (CBPP) is a lung disease caused by the bacterial pathogen Mycoplasma mycoides ssp. mycoides small colony type (MmmSC). It has been spreading due to a number of factors including poor vaccine efficacy and poor sensitivity of current diagnostic tests. The purpose of this study was to assess interferon gamma (IFN-gamma) release after stimulation of peripheral blood mononuclear cells (PBMC) from experimentally infected cattle. PBMC collected from 15 artificially infected animals were incubated with different concentrations of total MmmSC antigen. After 72h of incubation the IFN-gamma release was measured and found to be elevated in 11 animals. We did not observe a correlation between IFN-gamma release of animals with and without pathomorphological gross lesions. Therefore, our data do not confirm a role for CD4 T-lymphocytes in protection, since there is no correlation between IFN-g secretion (supposed to be mainly derived from CD4 T-cells) and disease severity. Additionally, we applied immunocytochemistry on affected lung tissue and detected no build up of T-lymphocytes (CD4 T-cells, CD8 T-cells) but a high presence of myeloid cells. |
| format | Journal Article |
| id | CGSpace1573 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2008 |
| publishDateRange | 2008 |
| publishDateSort | 2008 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace15732024-05-01T08:18:13Z Assessment of in vitro interferon-gamma responses from peripheral blood mononuclear cells of cattle infected with Mycoplasma mycoides ssp. mycoides small colony type Jores, Joerg Nkando, I. Sterner-Kock, Anja Haider, W. Poole, Elizabeth J. Unger, H. Muriuki, C. Wesonga, H.O. Taracha, E.L.N. animal diseases disease control Contagious bovine pleuropneumonia (CBPP) is a lung disease caused by the bacterial pathogen Mycoplasma mycoides ssp. mycoides small colony type (MmmSC). It has been spreading due to a number of factors including poor vaccine efficacy and poor sensitivity of current diagnostic tests. The purpose of this study was to assess interferon gamma (IFN-gamma) release after stimulation of peripheral blood mononuclear cells (PBMC) from experimentally infected cattle. PBMC collected from 15 artificially infected animals were incubated with different concentrations of total MmmSC antigen. After 72h of incubation the IFN-gamma release was measured and found to be elevated in 11 animals. We did not observe a correlation between IFN-gamma release of animals with and without pathomorphological gross lesions. Therefore, our data do not confirm a role for CD4 T-lymphocytes in protection, since there is no correlation between IFN-g secretion (supposed to be mainly derived from CD4 T-cells) and disease severity. Additionally, we applied immunocytochemistry on affected lung tissue and detected no build up of T-lymphocytes (CD4 T-cells, CD8 T-cells) but a high presence of myeloid cells. 2008-07 2010-05-18T21:15:37Z 2010-05-18T21:15:37Z Journal Article https://hdl.handle.net/10568/1573 en Limited Access Elsevier Jores, J.; Nkando, I.; Sterner-Kock, A.; Haider, W.; Poole, J.; Unger, H.; Muruiki, C.; Wesonga, H.; Taracha, E.L.N. 2008. Assessment of in vitro interferon-y responses from peripheral blood mononuclear cells of cattle infected with Mycoplasma mycoides ssp. mycoides small colony type. Veterinary Immunology and Immunopathology 124(1-2):192-197. |
| spellingShingle | animal diseases disease control Jores, Joerg Nkando, I. Sterner-Kock, Anja Haider, W. Poole, Elizabeth J. Unger, H. Muriuki, C. Wesonga, H.O. Taracha, E.L.N. Assessment of in vitro interferon-gamma responses from peripheral blood mononuclear cells of cattle infected with Mycoplasma mycoides ssp. mycoides small colony type |
| title | Assessment of in vitro interferon-gamma responses from peripheral blood mononuclear cells of cattle infected with Mycoplasma mycoides ssp. mycoides small colony type |
| title_full | Assessment of in vitro interferon-gamma responses from peripheral blood mononuclear cells of cattle infected with Mycoplasma mycoides ssp. mycoides small colony type |
| title_fullStr | Assessment of in vitro interferon-gamma responses from peripheral blood mononuclear cells of cattle infected with Mycoplasma mycoides ssp. mycoides small colony type |
| title_full_unstemmed | Assessment of in vitro interferon-gamma responses from peripheral blood mononuclear cells of cattle infected with Mycoplasma mycoides ssp. mycoides small colony type |
| title_short | Assessment of in vitro interferon-gamma responses from peripheral blood mononuclear cells of cattle infected with Mycoplasma mycoides ssp. mycoides small colony type |
| title_sort | assessment of in vitro interferon gamma responses from peripheral blood mononuclear cells of cattle infected with mycoplasma mycoides ssp mycoides small colony type |
| topic | animal diseases disease control |
| url | https://hdl.handle.net/10568/1573 |
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