Genome editing in apicomplexan parasites: Current status, challenges, and future possibilities
Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein (Cas) technology has revolutionized genome editing across various biological systems, including the Apicomplexa phylum. This review describes the status, challenges, and applications of CRISPR–Cas9 editing t...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Mary Ann Liebert Inc
2024
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| Acceso en línea: | https://hdl.handle.net/10568/155388 |
| _version_ | 1855522402157461504 |
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| author | Webi, Ethel Abkallo, Hussein M. Obiero, G. Ndegwa, P. Xie, S. Zhao, S. Nene, Vishvanath Steinaa, Lucilla |
| author_browse | Abkallo, Hussein M. Ndegwa, P. Nene, Vishvanath Obiero, G. Steinaa, Lucilla Webi, Ethel Xie, S. Zhao, S. |
| author_facet | Webi, Ethel Abkallo, Hussein M. Obiero, G. Ndegwa, P. Xie, S. Zhao, S. Nene, Vishvanath Steinaa, Lucilla |
| author_sort | Webi, Ethel |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein (Cas) technology has revolutionized genome editing across various biological systems, including the Apicomplexa phylum. This review describes the status, challenges, and applications of CRISPR–Cas9 editing technology in apicomplexan parasites, such as <i>Plasmodium</i>, <i>Toxoplasma</i>, <i>Theileria</i>, <i>Babesia</i>, and <i>Cryptosporidium</i>. The discussion encompasses successfully implemented CRISPR–Cas9-based techniques in these parasites, highlighting the achieved milestones, from precise gene modifications to genome-wide screening. In addition, the review addresses the challenges hampering efficient genome editing, including the parasites’ complex life cycles, multiple intracellular stages, and the lack of robust genetic tools. It further explores the ethical and policy considerations surrounding genome editing and the future perspectives of CRISPR–Cas applications in apicomplexan parasites. |
| format | Journal Article |
| id | CGSpace155388 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Mary Ann Liebert Inc |
| publisherStr | Mary Ann Liebert Inc |
| record_format | dspace |
| spelling | CGSpace1553882025-10-26T12:51:48Z Genome editing in apicomplexan parasites: Current status, challenges, and future possibilities Webi, Ethel Abkallo, Hussein M. Obiero, G. Ndegwa, P. Xie, S. Zhao, S. Nene, Vishvanath Steinaa, Lucilla crispr genomics Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein (Cas) technology has revolutionized genome editing across various biological systems, including the Apicomplexa phylum. This review describes the status, challenges, and applications of CRISPR–Cas9 editing technology in apicomplexan parasites, such as <i>Plasmodium</i>, <i>Toxoplasma</i>, <i>Theileria</i>, <i>Babesia</i>, and <i>Cryptosporidium</i>. The discussion encompasses successfully implemented CRISPR–Cas9-based techniques in these parasites, highlighting the achieved milestones, from precise gene modifications to genome-wide screening. In addition, the review addresses the challenges hampering efficient genome editing, including the parasites’ complex life cycles, multiple intracellular stages, and the lack of robust genetic tools. It further explores the ethical and policy considerations surrounding genome editing and the future perspectives of CRISPR–Cas applications in apicomplexan parasites. 2024-12-01 2024-10-17T10:28:05Z 2024-10-17T10:28:05Z Journal Article https://hdl.handle.net/10568/155388 en Open Access Mary Ann Liebert Inc Webi, E., Abkallo, H.M., Obiero, G., Ndegwa, P., Xie, S., Zhao, S., Nene, V. and Steinaa, L. 2024. Genome editing in apicomplexan parasites: Current status, challenges, and future possibilities. CRISPR Journal 7(6): 310–326. |
| spellingShingle | crispr genomics Webi, Ethel Abkallo, Hussein M. Obiero, G. Ndegwa, P. Xie, S. Zhao, S. Nene, Vishvanath Steinaa, Lucilla Genome editing in apicomplexan parasites: Current status, challenges, and future possibilities |
| title | Genome editing in apicomplexan parasites: Current status, challenges, and future possibilities |
| title_full | Genome editing in apicomplexan parasites: Current status, challenges, and future possibilities |
| title_fullStr | Genome editing in apicomplexan parasites: Current status, challenges, and future possibilities |
| title_full_unstemmed | Genome editing in apicomplexan parasites: Current status, challenges, and future possibilities |
| title_short | Genome editing in apicomplexan parasites: Current status, challenges, and future possibilities |
| title_sort | genome editing in apicomplexan parasites current status challenges and future possibilities |
| topic | crispr genomics |
| url | https://hdl.handle.net/10568/155388 |
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