Association of IL-4 and IL-10 maternal haplotypes with immune responses to P. falciparum in mothers and newborns
Particular cytokine gene polymorphisms are involved in the regulation of the antibody production. The consequences of already described IL-4, IL-10 and IL-13 gene polymorphisms on biological parameters and antibody levels were investigated among 576 mothers at delivery and their newborns in the cont...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2013
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/152968 |
| _version_ | 1855516376944345088 |
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| author | Lokossou, Adjimon Gatien Dechavanne, Célia Bouraïma, Aziz Courtin, David Le Port, Agnès Ladékpo, Rodolphe Noukpo, Julien Bonou, Désiré Ahouangninou, Claude Sabbagh, Audrey Fayomi, Benjamin Massougbodji, Achille Garcia, André Migot-Nabias, Florence |
| author_browse | Ahouangninou, Claude Bonou, Désiré Bouraïma, Aziz Courtin, David Dechavanne, Célia Fayomi, Benjamin Garcia, André Ladékpo, Rodolphe Le Port, Agnès Lokossou, Adjimon Gatien Massougbodji, Achille Migot-Nabias, Florence Noukpo, Julien Sabbagh, Audrey |
| author_facet | Lokossou, Adjimon Gatien Dechavanne, Célia Bouraïma, Aziz Courtin, David Le Port, Agnès Ladékpo, Rodolphe Noukpo, Julien Bonou, Désiré Ahouangninou, Claude Sabbagh, Audrey Fayomi, Benjamin Massougbodji, Achille Garcia, André Migot-Nabias, Florence |
| author_sort | Lokossou, Adjimon Gatien |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Particular cytokine gene polymorphisms are involved in the regulation of the antibody production. The consequences of already described IL-4, IL-10 and IL-13 gene polymorphisms on biological parameters and antibody levels were investigated among 576 mothers at delivery and their newborns in the context of P. falciparum placental malaria infection. The study took place in the semi-rural area of Tori-Bossito, in south-west Benin, where malaria is meso-endemic. Six biallelic polymorphisms were determined by quantitative PCR using TaqMan® Pre-Designed SNP Genotyping Assays, in IL-4 (rs2243250, rs2070874), IL-10 (rs1800896, rs1800871, rs1800872) and IL-13 (rs1800925) genes. Antibody responses directed to P. falciparum MSP-1, MSP-2, MSP-3, GLURP-R0, GLURP-R2 and AMA-1 recombinant proteins were determined by ELISA. The maternal IL-4 −590 *T/IL-4 +33 *T haplotype (one or two copies) was associated with favorable maternal condition at delivery (high haemoglobin levels, absence of placental parasites) and one of its component, the IL-4 −590 TT genotype, was related to low IgG levels to MSP-1, MSP-2/3D7 and MSP-2/FC27. Inversely, the maternal IL-10 −1082 AA was positively associated with P. falciparum placenta infection at delivery. As a consequence, the IL-10 −819 *T allele (in CT and TT genotypes) as well as the IL-10 −1082 *A/IL-10 −819 *T/IL-10 −592 *A haplotype (one or two copies) in which it is included, were related to an increased risk for anaemia in newborns. The maternal IL-10 −1082 AA genotype was related to high IgG levels to MSP-2/3D7 and AMA-1 in mothers and newborns, respectively. The IL-13 gene polymorphism was only involved in the newborn's antibody response to AMA-1. These data revealed that IL-4 and IL-10 maternal gene polymorphisms are likely to play a role in the regulation of biological parameters in pregnant women at delivery (anaemia, P. falciparum placenta infection) and in newborns (anaemia). Moreover, IL-4, IL-10 and IL-13 maternal gene polymorphisms were related to IgG responses to MSP-1, MSP-2/3D7 and MSP-2/FC27 in mothers as well as to AMA-1 in newborns. |
| format | Journal Article |
| id | CGSpace152968 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1529682025-09-25T13:01:37Z Association of IL-4 and IL-10 maternal haplotypes with immune responses to P. falciparum in mothers and newborns Lokossou, Adjimon Gatien Dechavanne, Célia Bouraïma, Aziz Courtin, David Le Port, Agnès Ladékpo, Rodolphe Noukpo, Julien Bonou, Désiré Ahouangninou, Claude Sabbagh, Audrey Fayomi, Benjamin Massougbodji, Achille Garcia, André Migot-Nabias, Florence malaria epidemiology children Particular cytokine gene polymorphisms are involved in the regulation of the antibody production. The consequences of already described IL-4, IL-10 and IL-13 gene polymorphisms on biological parameters and antibody levels were investigated among 576 mothers at delivery and their newborns in the context of P. falciparum placental malaria infection. The study took place in the semi-rural area of Tori-Bossito, in south-west Benin, where malaria is meso-endemic. Six biallelic polymorphisms were determined by quantitative PCR using TaqMan® Pre-Designed SNP Genotyping Assays, in IL-4 (rs2243250, rs2070874), IL-10 (rs1800896, rs1800871, rs1800872) and IL-13 (rs1800925) genes. Antibody responses directed to P. falciparum MSP-1, MSP-2, MSP-3, GLURP-R0, GLURP-R2 and AMA-1 recombinant proteins were determined by ELISA. The maternal IL-4 −590 *T/IL-4 +33 *T haplotype (one or two copies) was associated with favorable maternal condition at delivery (high haemoglobin levels, absence of placental parasites) and one of its component, the IL-4 −590 TT genotype, was related to low IgG levels to MSP-1, MSP-2/3D7 and MSP-2/FC27. Inversely, the maternal IL-10 −1082 AA was positively associated with P. falciparum placenta infection at delivery. As a consequence, the IL-10 −819 *T allele (in CT and TT genotypes) as well as the IL-10 −1082 *A/IL-10 −819 *T/IL-10 −592 *A haplotype (one or two copies) in which it is included, were related to an increased risk for anaemia in newborns. The maternal IL-10 −1082 AA genotype was related to high IgG levels to MSP-2/3D7 and AMA-1 in mothers and newborns, respectively. The IL-13 gene polymorphism was only involved in the newborn's antibody response to AMA-1. These data revealed that IL-4 and IL-10 maternal gene polymorphisms are likely to play a role in the regulation of biological parameters in pregnant women at delivery (anaemia, P. falciparum placenta infection) and in newborns (anaemia). Moreover, IL-4, IL-10 and IL-13 maternal gene polymorphisms were related to IgG responses to MSP-1, MSP-2/3D7 and MSP-2/FC27 in mothers as well as to AMA-1 in newborns. 2013-12 2024-10-01T13:55:24Z 2024-10-01T13:55:24Z Journal Article https://hdl.handle.net/10568/152968 en Open Access Springer Lokossou, Adjimon Gatien; Dechavanne, Célia; Bouraïma, Aziz; Courtin, David; Le Port, Agnès; Ladékpo, Rodolphe; Noukpo, Julien; Bonou, Désiré; Ahouangninou, Claude; Sabbagh, Audrey; Fayomi, Benjamin; Massougbodji, Achille; Garcia, André; and Migot-Nabias, Florence. 2013. Association of IL-4 and IL-10 maternal haplotypes with immune responses to P. falciparum in mothers and newborns. BMC Infectious Diseases 13: 215. https://doi.org/10.1186/1471-2334-13-215 |
| spellingShingle | malaria epidemiology children Lokossou, Adjimon Gatien Dechavanne, Célia Bouraïma, Aziz Courtin, David Le Port, Agnès Ladékpo, Rodolphe Noukpo, Julien Bonou, Désiré Ahouangninou, Claude Sabbagh, Audrey Fayomi, Benjamin Massougbodji, Achille Garcia, André Migot-Nabias, Florence Association of IL-4 and IL-10 maternal haplotypes with immune responses to P. falciparum in mothers and newborns |
| title | Association of IL-4 and IL-10 maternal haplotypes with immune responses to P. falciparum in mothers and newborns |
| title_full | Association of IL-4 and IL-10 maternal haplotypes with immune responses to P. falciparum in mothers and newborns |
| title_fullStr | Association of IL-4 and IL-10 maternal haplotypes with immune responses to P. falciparum in mothers and newborns |
| title_full_unstemmed | Association of IL-4 and IL-10 maternal haplotypes with immune responses to P. falciparum in mothers and newborns |
| title_short | Association of IL-4 and IL-10 maternal haplotypes with immune responses to P. falciparum in mothers and newborns |
| title_sort | association of il 4 and il 10 maternal haplotypes with immune responses to p falciparum in mothers and newborns |
| topic | malaria epidemiology children |
| url | https://hdl.handle.net/10568/152968 |
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