Brucella species circulating in smallholder dairy cattle in Tanzania

Brucellosis is a zoonosis caused by bacteria of the genus <i>Brucella</i>, which results in economic losses relating to livestock and threatens public health. A cross-sectional study was conducted to determine the molecular prevalence of <i>Brucella</i> species in smallholder dairy cattle in six reg...

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Main Authors: Mengele, Isaac J., Akoko, James M., Shirima, G.M., Bwatota, S.F., Motto, S.K., Hernandez-Castro, L.E., Komwihangilo, D.M., Lyatuu, Eliamoni, Bronsvoort, B.M. de C., Cook, Elizabeth A.J.
Format: Journal Article
Language:Inglés
Published: MDPI 2024
Subjects:
Online Access:https://hdl.handle.net/10568/152333
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author Mengele, Isaac J.
Akoko, James M.
Shirima, G.M.
Bwatota, S.F.
Motto, S.K.
Hernandez-Castro, L.E.
Komwihangilo, D.M.
Lyatuu, Eliamoni
Bronsvoort, B.M. de C.
Cook, Elizabeth A.J.
author_browse Akoko, James M.
Bronsvoort, B.M. de C.
Bwatota, S.F.
Cook, Elizabeth A.J.
Hernandez-Castro, L.E.
Komwihangilo, D.M.
Lyatuu, Eliamoni
Mengele, Isaac J.
Motto, S.K.
Shirima, G.M.
author_facet Mengele, Isaac J.
Akoko, James M.
Shirima, G.M.
Bwatota, S.F.
Motto, S.K.
Hernandez-Castro, L.E.
Komwihangilo, D.M.
Lyatuu, Eliamoni
Bronsvoort, B.M. de C.
Cook, Elizabeth A.J.
author_sort Mengele, Isaac J.
collection Repository of Agricultural Research Outputs (CGSpace)
description Brucellosis is a zoonosis caused by bacteria of the genus <i>Brucella</i>, which results in economic losses relating to livestock and threatens public health. A cross-sectional study was conducted to determine the molecular prevalence of <i>Brucella</i> species in smallholder dairy cattle in six regions of Tanzania from July 2019 to October 2020. Dairy cattle (n = 2048) were sampled from 1371 farms. DNA extracted from blood and vaginal swabs was tested for <i>Brucella</i> using qPCR targeting the IS711 gene and positives were tested for the alkB marker for <i>B. abortus</i> and BMEI1172 marker for <i>B. melitensis</i>. The molecular prevalence was 3.5% (95% CI: 2.8–4.4) with the highest prevalence 8.1% (95% CI: 4.6–13.0) in Njombe region. <i>B. melitensis</i> was the predominant species detected (66.2%). Further studies are recommended to understand the source of <i>B. melitensis</i> and its implications for veterinary public health. Livestock keepers should be informed of the risks and biosecurity practices to reduce the introduction and control of <i>Brucella</i>. Cattle and small ruminant vaccination programs could be implemented to control brucellosis in high-risk populations in the country.
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spelling CGSpace1523332025-12-08T10:29:22Z Brucella species circulating in smallholder dairy cattle in Tanzania Mengele, Isaac J. Akoko, James M. Shirima, G.M. Bwatota, S.F. Motto, S.K. Hernandez-Castro, L.E. Komwihangilo, D.M. Lyatuu, Eliamoni Bronsvoort, B.M. de C. Cook, Elizabeth A.J. brucellosis cattle dairying zoonoses Brucellosis is a zoonosis caused by bacteria of the genus <i>Brucella</i>, which results in economic losses relating to livestock and threatens public health. A cross-sectional study was conducted to determine the molecular prevalence of <i>Brucella</i> species in smallholder dairy cattle in six regions of Tanzania from July 2019 to October 2020. Dairy cattle (n = 2048) were sampled from 1371 farms. DNA extracted from blood and vaginal swabs was tested for <i>Brucella</i> using qPCR targeting the IS711 gene and positives were tested for the alkB marker for <i>B. abortus</i> and BMEI1172 marker for <i>B. melitensis</i>. The molecular prevalence was 3.5% (95% CI: 2.8–4.4) with the highest prevalence 8.1% (95% CI: 4.6–13.0) in Njombe region. <i>B. melitensis</i> was the predominant species detected (66.2%). Further studies are recommended to understand the source of <i>B. melitensis</i> and its implications for veterinary public health. Livestock keepers should be informed of the risks and biosecurity practices to reduce the introduction and control of <i>Brucella</i>. Cattle and small ruminant vaccination programs could be implemented to control brucellosis in high-risk populations in the country. 2024-09-21 2024-09-23T06:53:11Z 2024-09-23T06:53:11Z Journal Article https://hdl.handle.net/10568/152333 en Open Access MDPI Mengele, I.J., Akoko, J.M., Shirima, G.M., Bwatota, S.F., Motto, S.K., Hernandez-Castro, L.E., Komwihangilo, D.M., Lyatuu, E., Bronsvoort, B.M. de C. and Cook, E.A.J. 2024. <i>Brucella</i> species circulating in smallholder dairy cattle in Tanzania. Pathogens 13(9): 815.
spellingShingle brucellosis
cattle
dairying
zoonoses
Mengele, Isaac J.
Akoko, James M.
Shirima, G.M.
Bwatota, S.F.
Motto, S.K.
Hernandez-Castro, L.E.
Komwihangilo, D.M.
Lyatuu, Eliamoni
Bronsvoort, B.M. de C.
Cook, Elizabeth A.J.
Brucella species circulating in smallholder dairy cattle in Tanzania
title Brucella species circulating in smallholder dairy cattle in Tanzania
title_full Brucella species circulating in smallholder dairy cattle in Tanzania
title_fullStr Brucella species circulating in smallholder dairy cattle in Tanzania
title_full_unstemmed Brucella species circulating in smallholder dairy cattle in Tanzania
title_short Brucella species circulating in smallholder dairy cattle in Tanzania
title_sort brucella species circulating in smallholder dairy cattle in tanzania
topic brucellosis
cattle
dairying
zoonoses
url https://hdl.handle.net/10568/152333
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