Morphological, pathological and phylogenetic analyses identify a diverse group of Colletotrichum spp. causing leaf, pod and flower diseases on the orphan legume African yam bean
African yam bean (AYB; Sphenostylis stenocarpa) is an underutilized legume indigenous to Africa with great potential to enhance food security and offer nutritional and medicinal opportunities. However, low grain yield caused by fungal diseases, including pod blight and leaf tip dieback, deters farme...
| Autores principales: | , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2024
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/152327 |
| _version_ | 1855542851303112704 |
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| author | Ogunsanya, O.M. Adebisi, M.A. Popoola, A.R. Afolabi, C.G. Oyatomi, O. Colgan, R. Armitage, A. Thompson, E. Abberton, M. Ortega-Beltran, A. |
| author_browse | Abberton, M. Adebisi, M.A. Afolabi, C.G. Armitage, A. Colgan, R. Ogunsanya, O.M. Ortega-Beltran, A. Oyatomi, O. Popoola, A.R. Thompson, E. |
| author_facet | Ogunsanya, O.M. Adebisi, M.A. Popoola, A.R. Afolabi, C.G. Oyatomi, O. Colgan, R. Armitage, A. Thompson, E. Abberton, M. Ortega-Beltran, A. |
| author_sort | Ogunsanya, O.M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | African yam bean (AYB; Sphenostylis stenocarpa) is an underutilized legume indigenous to Africa with great potential to enhance food security and offer nutritional and medicinal opportunities. However, low grain yield caused by fungal diseases, including pod blight and leaf tip dieback, deters farmers from large‐scale cultivation. To determine the prevalence of fungal diseases affecting leaves, pods and flowers of AYB, a survey was conducted in 2018 and 2019 in major AYB‐growing areas in Nigeria. Leaf tip dieback, flower bud rot and pod blight were the most common symptoms. Morphological and molecular assays were conducted to identify the causal agents of the observed diseases. In all the samples examined, fungi from eight genera were isolated from diseased leaves, buds and pods. Koch's postulates were fulfilled only for fungi belonging to the Colletotrichum genus. Fungi from the other seven genera did not produce disease symptoms in healthy AYB tissues. Several Colletotrichum isolates were characterized by sequencing the rDNA internal transcribed spacer (ITS), glyceraldehyde‐3‐phosphate dehydrogenase, calmodulin and ApMAT loci. A combined phylogenetic analysis revealed four Colletotrichum species: C. siamense, C. theobromicola and C. fructicola, which were recovered from diseased leaves, and C. truncatum, recovered from diseased pods and buds. Our results are useful to gear efforts to develop integrated management strategies to control diseases affecting AYB in Nigeria and elsewhere. Availability of such strategies may stimulate greater AYB cultivation, which can contribute to diet diversification, something repeatedly advocated by a range of stakeholders to increase food security and prosperity of smallholder farmers. |
| format | Journal Article |
| id | CGSpace152327 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1523272025-11-11T11:07:28Z Morphological, pathological and phylogenetic analyses identify a diverse group of Colletotrichum spp. causing leaf, pod and flower diseases on the orphan legume African yam bean Ogunsanya, O.M. Adebisi, M.A. Popoola, A.R. Afolabi, C.G. Oyatomi, O. Colgan, R. Armitage, A. Thompson, E. Abberton, M. Ortega-Beltran, A. african yam beans integrated management plant diseases smallholder farmers African yam bean (AYB; Sphenostylis stenocarpa) is an underutilized legume indigenous to Africa with great potential to enhance food security and offer nutritional and medicinal opportunities. However, low grain yield caused by fungal diseases, including pod blight and leaf tip dieback, deters farmers from large‐scale cultivation. To determine the prevalence of fungal diseases affecting leaves, pods and flowers of AYB, a survey was conducted in 2018 and 2019 in major AYB‐growing areas in Nigeria. Leaf tip dieback, flower bud rot and pod blight were the most common symptoms. Morphological and molecular assays were conducted to identify the causal agents of the observed diseases. In all the samples examined, fungi from eight genera were isolated from diseased leaves, buds and pods. Koch's postulates were fulfilled only for fungi belonging to the Colletotrichum genus. Fungi from the other seven genera did not produce disease symptoms in healthy AYB tissues. Several Colletotrichum isolates were characterized by sequencing the rDNA internal transcribed spacer (ITS), glyceraldehyde‐3‐phosphate dehydrogenase, calmodulin and ApMAT loci. A combined phylogenetic analysis revealed four Colletotrichum species: C. siamense, C. theobromicola and C. fructicola, which were recovered from diseased leaves, and C. truncatum, recovered from diseased pods and buds. Our results are useful to gear efforts to develop integrated management strategies to control diseases affecting AYB in Nigeria and elsewhere. Availability of such strategies may stimulate greater AYB cultivation, which can contribute to diet diversification, something repeatedly advocated by a range of stakeholders to increase food security and prosperity of smallholder farmers. 2024-12 2024-09-20T11:05:05Z 2024-09-20T11:05:05Z Journal Article https://hdl.handle.net/10568/152327 en Open Access application/pdf Wiley Ogunsanya, O.M., Adebisi, M.A., Popoola, A.R., Afolabi, C.G., Oyatomi, O., Colgan, R., ... & Ortega‐Beltran, A. (2024). Morphological, pathological and phylogenetic analyses identify a diverse group of Colletotrichum spp. causing leaf, pod and flower diseases on the orphan legume African yam bean. Plant Pathology, 1-15. |
| spellingShingle | african yam beans integrated management plant diseases smallholder farmers Ogunsanya, O.M. Adebisi, M.A. Popoola, A.R. Afolabi, C.G. Oyatomi, O. Colgan, R. Armitage, A. Thompson, E. Abberton, M. Ortega-Beltran, A. Morphological, pathological and phylogenetic analyses identify a diverse group of Colletotrichum spp. causing leaf, pod and flower diseases on the orphan legume African yam bean |
| title | Morphological, pathological and phylogenetic analyses identify a diverse group of Colletotrichum spp. causing leaf, pod and flower diseases on the orphan legume African yam bean |
| title_full | Morphological, pathological and phylogenetic analyses identify a diverse group of Colletotrichum spp. causing leaf, pod and flower diseases on the orphan legume African yam bean |
| title_fullStr | Morphological, pathological and phylogenetic analyses identify a diverse group of Colletotrichum spp. causing leaf, pod and flower diseases on the orphan legume African yam bean |
| title_full_unstemmed | Morphological, pathological and phylogenetic analyses identify a diverse group of Colletotrichum spp. causing leaf, pod and flower diseases on the orphan legume African yam bean |
| title_short | Morphological, pathological and phylogenetic analyses identify a diverse group of Colletotrichum spp. causing leaf, pod and flower diseases on the orphan legume African yam bean |
| title_sort | morphological pathological and phylogenetic analyses identify a diverse group of colletotrichum spp causing leaf pod and flower diseases on the orphan legume african yam bean |
| topic | african yam beans integrated management plant diseases smallholder farmers |
| url | https://hdl.handle.net/10568/152327 |
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