Isolation, genome analysis and tissue localization of ceratobasidium theobromae, a new encounter pathogen of cassava in Southeast Asia
In Southeast Asia (SEA) fastidious fungi of the Ceratobasidium genus are associated with proliferation of sprouts and vascular necrosis in cacao and cassava, crops that were introduced from the tropical Americas to this region. Here, we report the isolation and in vitro culture of a Ceratobasidium s...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Nature Portfolio
2024
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/152318 |
| _version_ | 1855529765033738240 |
|---|---|
| author | Gil-Ordóñez, Alejandra Pardo, Juan M Sheat, Samar Xaiyavong, Khamla Leiva, Ana M. Arinaitwe, Warren Winter, Stephan Newby, Jonathan Cuellar, Wilmer J. |
| author_browse | Arinaitwe, Warren Cuellar, Wilmer J. Gil-Ordóñez, Alejandra Leiva, Ana M. Newby, Jonathan Pardo, Juan M Sheat, Samar Winter, Stephan Xaiyavong, Khamla |
| author_facet | Gil-Ordóñez, Alejandra Pardo, Juan M Sheat, Samar Xaiyavong, Khamla Leiva, Ana M. Arinaitwe, Warren Winter, Stephan Newby, Jonathan Cuellar, Wilmer J. |
| author_sort | Gil-Ordóñez, Alejandra |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | In Southeast Asia (SEA) fastidious fungi of the Ceratobasidium genus are associated with proliferation of sprouts and vascular necrosis in cacao and cassava, crops that were introduced from the tropical Americas to this region. Here, we report the isolation and in vitro culture of a Ceratobasidium sp. isolated from cassava with symptoms of witches' broom disease (CWBD), a devastating disease of this crop in SEA. The genome characterization using a hybrid assembly strategy identifies the fungus as an isolate of the species C. theobromae, the causal agent of vascular streak dieback of cacao in SEA. Both fungi have a genome size > 31 Mb (G+C content 49%), share > 98% nucleotide identity of the Internal Transcribed Spacer (ITS) and > 94% in genes used for species-level identification. Using RNAscope® we traced the pathogen and confirmed its irregular distribution in the xylem and epidermis along the cassava stem, which explains the obtention of healthy planting material from symptom-free parts of a diseased plant. These results are essential for understanding the epidemiology of CWBD, as a basis for disease management including measures to prevent further spread and minimize the risk of introducing C. theobromae via long-distance movement of cassava materials to Africa and the Americas. |
| format | Journal Article |
| id | CGSpace152318 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Nature Portfolio |
| publisherStr | Nature Portfolio |
| record_format | dspace |
| spelling | CGSpace1523182025-12-08T10:06:44Z Isolation, genome analysis and tissue localization of ceratobasidium theobromae, a new encounter pathogen of cassava in Southeast Asia Gil-Ordóñez, Alejandra Pardo, Juan M Sheat, Samar Xaiyavong, Khamla Leiva, Ana M. Arinaitwe, Warren Winter, Stephan Newby, Jonathan Cuellar, Wilmer J. cassava pathogens disease management ceratobasidium genus In Southeast Asia (SEA) fastidious fungi of the Ceratobasidium genus are associated with proliferation of sprouts and vascular necrosis in cacao and cassava, crops that were introduced from the tropical Americas to this region. Here, we report the isolation and in vitro culture of a Ceratobasidium sp. isolated from cassava with symptoms of witches' broom disease (CWBD), a devastating disease of this crop in SEA. The genome characterization using a hybrid assembly strategy identifies the fungus as an isolate of the species C. theobromae, the causal agent of vascular streak dieback of cacao in SEA. Both fungi have a genome size > 31 Mb (G+C content 49%), share > 98% nucleotide identity of the Internal Transcribed Spacer (ITS) and > 94% in genes used for species-level identification. Using RNAscope® we traced the pathogen and confirmed its irregular distribution in the xylem and epidermis along the cassava stem, which explains the obtention of healthy planting material from symptom-free parts of a diseased plant. These results are essential for understanding the epidemiology of CWBD, as a basis for disease management including measures to prevent further spread and minimize the risk of introducing C. theobromae via long-distance movement of cassava materials to Africa and the Americas. 2024-08-05 2024-09-19T21:08:26Z 2024-09-19T21:08:26Z Journal Article https://hdl.handle.net/10568/152318 en Open Access application/pdf Nature Portfolio Gil-Ordóñez, A.; Pardo, J.M.; Sheat, S.; Xaiyavong, K.; Leiva, A.M.; Arinaitwe, W.; Winter, S.; Newby, J.; Cuellar, W.J. (2024) Isolation, genome analysis and tissue localization of ceratobasidium theobromae, a new encounter pathogen of cassava in Southeast Asia. Scientific Reports 14(1): 18139. ISSN: 2045-2322 |
| spellingShingle | cassava pathogens disease management ceratobasidium genus Gil-Ordóñez, Alejandra Pardo, Juan M Sheat, Samar Xaiyavong, Khamla Leiva, Ana M. Arinaitwe, Warren Winter, Stephan Newby, Jonathan Cuellar, Wilmer J. Isolation, genome analysis and tissue localization of ceratobasidium theobromae, a new encounter pathogen of cassava in Southeast Asia |
| title | Isolation, genome analysis and tissue localization of ceratobasidium theobromae, a new encounter pathogen of cassava in Southeast Asia |
| title_full | Isolation, genome analysis and tissue localization of ceratobasidium theobromae, a new encounter pathogen of cassava in Southeast Asia |
| title_fullStr | Isolation, genome analysis and tissue localization of ceratobasidium theobromae, a new encounter pathogen of cassava in Southeast Asia |
| title_full_unstemmed | Isolation, genome analysis and tissue localization of ceratobasidium theobromae, a new encounter pathogen of cassava in Southeast Asia |
| title_short | Isolation, genome analysis and tissue localization of ceratobasidium theobromae, a new encounter pathogen of cassava in Southeast Asia |
| title_sort | isolation genome analysis and tissue localization of ceratobasidium theobromae a new encounter pathogen of cassava in southeast asia |
| topic | cassava pathogens disease management ceratobasidium genus |
| url | https://hdl.handle.net/10568/152318 |
| work_keys_str_mv | AT gilordonezalejandra isolationgenomeanalysisandtissuelocalizationofceratobasidiumtheobromaeanewencounterpathogenofcassavainsoutheastasia AT pardojuanm isolationgenomeanalysisandtissuelocalizationofceratobasidiumtheobromaeanewencounterpathogenofcassavainsoutheastasia AT sheatsamar isolationgenomeanalysisandtissuelocalizationofceratobasidiumtheobromaeanewencounterpathogenofcassavainsoutheastasia AT xaiyavongkhamla isolationgenomeanalysisandtissuelocalizationofceratobasidiumtheobromaeanewencounterpathogenofcassavainsoutheastasia AT leivaanam isolationgenomeanalysisandtissuelocalizationofceratobasidiumtheobromaeanewencounterpathogenofcassavainsoutheastasia AT arinaitwewarren isolationgenomeanalysisandtissuelocalizationofceratobasidiumtheobromaeanewencounterpathogenofcassavainsoutheastasia AT winterstephan isolationgenomeanalysisandtissuelocalizationofceratobasidiumtheobromaeanewencounterpathogenofcassavainsoutheastasia AT newbyjonathan isolationgenomeanalysisandtissuelocalizationofceratobasidiumtheobromaeanewencounterpathogenofcassavainsoutheastasia AT cuellarwilmerj isolationgenomeanalysisandtissuelocalizationofceratobasidiumtheobromaeanewencounterpathogenofcassavainsoutheastasia |