Endoplasmic reticulum contains a common, abundant calcium-binding glycoprotein, endoplasmin
The most abundant protein in microsomal membrane preparations from mammalian cells has been identified as a 100 × 103Mr concanavalin A-binding glycoprotein. The glycosyl moiety of the glycoprotein is completely sensitive to endoglycosidase H, suggesting a predominantly endoplasmic reticulum localiza...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Company of Biologists
1986
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/152106 |
| _version_ | 1855539202106589184 |
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| author | Koch, G. Smith, M. Macer, D. Webster, P. Mortara, R. |
| author_browse | Koch, G. Macer, D. Mortara, R. Smith, M. Webster, P. |
| author_facet | Koch, G. Smith, M. Macer, D. Webster, P. Mortara, R. |
| author_sort | Koch, G. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The most abundant protein in microsomal membrane preparations from mammalian cells has been identified as a 100 × 103Mr concanavalin A-binding glycoprotein. The glycosyl moiety of the glycoprotein is completely sensitive to endoglycosidase H, suggesting a predominantly endoplasmic reticulum localization in the cell. Using a monospecific antibody it was shown by binding and immunofluorescence studies that the glycoprotein is intracellular. Immunoelectron microscopy showed that the glycoprotein was at least 100 times more concentrated in the endoplasmic reticulum than in any other cellular organelle. It was found to be substantially overexpressed in cells and tissues rich in endoplasmic reticulum. Since it is the major common protein component associated with the endoplasmic reticulum we refer to it as endoplasmin. Calcium-binding studies show that endoplasmin is a major calcium-binding protein in cells, suggesting that at least one of its roles might be in the calcium-storage function of the endoplasmic reticulum. The amino-terminal sequence of endoplasmin is identical to that of a 100 × 103Mr stress-related protein. |
| format | Journal Article |
| id | CGSpace152106 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1986 |
| publishDateRange | 1986 |
| publishDateSort | 1986 |
| publisher | Company of Biologists |
| publisherStr | Company of Biologists |
| record_format | dspace |
| spelling | CGSpace1521062025-12-08T10:29:22Z Endoplasmic reticulum contains a common, abundant calcium-binding glycoprotein, endoplasmin Koch, G. Smith, M. Macer, D. Webster, P. Mortara, R. stress calcium cells protein The most abundant protein in microsomal membrane preparations from mammalian cells has been identified as a 100 × 103Mr concanavalin A-binding glycoprotein. The glycosyl moiety of the glycoprotein is completely sensitive to endoglycosidase H, suggesting a predominantly endoplasmic reticulum localization in the cell. Using a monospecific antibody it was shown by binding and immunofluorescence studies that the glycoprotein is intracellular. Immunoelectron microscopy showed that the glycoprotein was at least 100 times more concentrated in the endoplasmic reticulum than in any other cellular organelle. It was found to be substantially overexpressed in cells and tissues rich in endoplasmic reticulum. Since it is the major common protein component associated with the endoplasmic reticulum we refer to it as endoplasmin. Calcium-binding studies show that endoplasmin is a major calcium-binding protein in cells, suggesting that at least one of its roles might be in the calcium-storage function of the endoplasmic reticulum. The amino-terminal sequence of endoplasmin is identical to that of a 100 × 103Mr stress-related protein. 1986-12-01 2024-09-11T09:26:00Z 2024-09-11T09:26:00Z Journal Article https://hdl.handle.net/10568/152106 en Limited Access Company of Biologists Koch, G., Smith, M., Macer, D., Webster, P., & Mortara, R. (1986). Endoplasmic reticulum contains a common, abundant calcium-binding glycoprotein, endoplasmin. Journal of Cell Science, 86(1), 217–232. https://doi.org/10.1242/jcs.86.1.217 |
| spellingShingle | stress calcium cells protein Koch, G. Smith, M. Macer, D. Webster, P. Mortara, R. Endoplasmic reticulum contains a common, abundant calcium-binding glycoprotein, endoplasmin |
| title | Endoplasmic reticulum contains a common, abundant calcium-binding glycoprotein, endoplasmin |
| title_full | Endoplasmic reticulum contains a common, abundant calcium-binding glycoprotein, endoplasmin |
| title_fullStr | Endoplasmic reticulum contains a common, abundant calcium-binding glycoprotein, endoplasmin |
| title_full_unstemmed | Endoplasmic reticulum contains a common, abundant calcium-binding glycoprotein, endoplasmin |
| title_short | Endoplasmic reticulum contains a common, abundant calcium-binding glycoprotein, endoplasmin |
| title_sort | endoplasmic reticulum contains a common abundant calcium binding glycoprotein endoplasmin |
| topic | stress calcium cells protein |
| url | https://hdl.handle.net/10568/152106 |
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