Direct in vitro propagation of avian germ cells from an embryonic gonad biorepository
Direct introduction of cryopreserved embryonic gonadal germ cells (GGC) into a sterile chicken surrogate host to reconstitute a chicken breed has been demonstrated as a feasible approach for preserving and utilizing chicken genetic resources. This method is highly efficient using male gonads; howeve...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/152042 |
| _version_ | 1855539715978035200 |
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| author | Tuanjun Hu Purdy, P.H. Blank, M.H. Muhonja, Christine Kamidi Pereira, R.J.G. Tiambo, Christian K. |
| author_browse | Blank, M.H. Muhonja, Christine Kamidi Pereira, R.J.G. Purdy, P.H. Tiambo, Christian K. Tuanjun Hu |
| author_facet | Tuanjun Hu Purdy, P.H. Blank, M.H. Muhonja, Christine Kamidi Pereira, R.J.G. Tiambo, Christian K. |
| author_sort | Tuanjun Hu |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Direct introduction of cryopreserved embryonic gonadal germ cells (GGC) into a sterile chicken surrogate host to reconstitute a chicken breed has been demonstrated as a feasible approach for preserving and utilizing chicken genetic resources. This method is highly efficient using male gonads; however, a large number of frozen female embryonic gonads is needed to provide sufficient purified GGC for the generation of fertile surrogate female hosts. Applying this method to indigenous chicken breeds and other bird species is difficult due to small flock numbers and poor egg production in each egg laying cycle. Propagating germ cells from the frozen gonadal tissues may be a solution for the biobanking of these birds. Here, we describe a simplified method for culture of GGC from frozen embryonic 9.5 d gonads. At this developmental stage, the germ cells are autonomously shed into medium, yielding hundreds to thousands of mitosis-competent germ cells. The resulting cultures of GGC have over 90% purity, uniformly express SSEA-1 and DAZL antigens and can re-colonize recipient's gonads. The GGC recovery rate from frozen gonads are 42% to 100%, depending on length of cryopreservation and the breed or line of chickens. Entire chicken embryos can also be directly cryopreserved for later gonadal isolation and culture. This storage method is a supplementary approach to safeguard local indigenous chicken breeds bearing valuable genetic traits and should be applicable to the biobanking of many bird species. |
| format | Journal Article |
| id | CGSpace152042 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1520422025-10-26T12:50:57Z Direct in vitro propagation of avian germ cells from an embryonic gonad biorepository Tuanjun Hu Purdy, P.H. Blank, M.H. Muhonja, Christine Kamidi Pereira, R.J.G. Tiambo, Christian K. indigenous breeds chickens animal breeding breeds poultry Direct introduction of cryopreserved embryonic gonadal germ cells (GGC) into a sterile chicken surrogate host to reconstitute a chicken breed has been demonstrated as a feasible approach for preserving and utilizing chicken genetic resources. This method is highly efficient using male gonads; however, a large number of frozen female embryonic gonads is needed to provide sufficient purified GGC for the generation of fertile surrogate female hosts. Applying this method to indigenous chicken breeds and other bird species is difficult due to small flock numbers and poor egg production in each egg laying cycle. Propagating germ cells from the frozen gonadal tissues may be a solution for the biobanking of these birds. Here, we describe a simplified method for culture of GGC from frozen embryonic 9.5 d gonads. At this developmental stage, the germ cells are autonomously shed into medium, yielding hundreds to thousands of mitosis-competent germ cells. The resulting cultures of GGC have over 90% purity, uniformly express SSEA-1 and DAZL antigens and can re-colonize recipient's gonads. The GGC recovery rate from frozen gonads are 42% to 100%, depending on length of cryopreservation and the breed or line of chickens. Entire chicken embryos can also be directly cryopreserved for later gonadal isolation and culture. This storage method is a supplementary approach to safeguard local indigenous chicken breeds bearing valuable genetic traits and should be applicable to the biobanking of many bird species. 2024-11 2024-09-09T07:03:22Z 2024-09-09T07:03:22Z Journal Article https://hdl.handle.net/10568/152042 en Open Access Elsevier Tuanjun Hu, Purdy, P.H., Blank, M.H., Muhonja, C.K., Pereira, R.J.G., Tiambo, C.K. and McGrew, M.J. 2024. Direct in vitro propagation of avian germ cells from an embryonic gonad biorepository. Poultry Science 103(11):104260. |
| spellingShingle | indigenous breeds chickens animal breeding breeds poultry Tuanjun Hu Purdy, P.H. Blank, M.H. Muhonja, Christine Kamidi Pereira, R.J.G. Tiambo, Christian K. Direct in vitro propagation of avian germ cells from an embryonic gonad biorepository |
| title | Direct in vitro propagation of avian germ cells from an embryonic gonad biorepository |
| title_full | Direct in vitro propagation of avian germ cells from an embryonic gonad biorepository |
| title_fullStr | Direct in vitro propagation of avian germ cells from an embryonic gonad biorepository |
| title_full_unstemmed | Direct in vitro propagation of avian germ cells from an embryonic gonad biorepository |
| title_short | Direct in vitro propagation of avian germ cells from an embryonic gonad biorepository |
| title_sort | direct in vitro propagation of avian germ cells from an embryonic gonad biorepository |
| topic | indigenous breeds chickens animal breeding breeds poultry |
| url | https://hdl.handle.net/10568/152042 |
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