Genome-wide association studies reveals new candidate genes associated with resistance to Striga gesneroides in Cowpea [Vigna unguiculata (L.) Walp.] accessions from sub-Saharan Africa
Parasitic plant Striga gesneroides reduces cowpea productivity in sub-Saharan Africa, leading to substantial yield losses. This weed is the major reason for land abandonment by many farmers. While various methods have been proposed for managing Striga invasion and parasitism, host-plant resistance r...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
2024
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/151959 |
| _version_ | 1855517740146622464 |
|---|---|
| author | Koura, A.A. Kena, A.W. Annor, B. Adejumobi, I.I. Sayadi Maazou, A.R. Awuku, F.J. Attamah, P. Boukar, O. Akromah, R. |
| author_browse | Adejumobi, I.I. Akromah, R. Annor, B. Attamah, P. Awuku, F.J. Boukar, O. Kena, A.W. Koura, A.A. Sayadi Maazou, A.R. |
| author_facet | Koura, A.A. Kena, A.W. Annor, B. Adejumobi, I.I. Sayadi Maazou, A.R. Awuku, F.J. Attamah, P. Boukar, O. Akromah, R. |
| author_sort | Koura, A.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Parasitic plant Striga gesneroides reduces cowpea productivity in sub-Saharan Africa, leading to substantial yield losses. This weed is the major reason for land abandonment by many farmers. While various methods have been proposed for managing Striga invasion and parasitism, host-plant resistance remains the most effective and affordable option for small-scale farmers in the sub-region. This study aims to expedite the improvement of Striga resistance breeding through marker-assisted selection by identifying genomic regions and candidate genes associated with Striga resistance indicator traits using association mapping. A panel of 188 cowpea accessions assembled from five gene banks in West Africa were phenotyped for two years under Striga-infested and non-infested research conditions. These accessions were equally genotyped using the medium-density genotyping of the Diversity Arrays Technology sequencing (DArTseq) platform to obtain marker information. High broad-sense heritability estimates were observed for Striga resistance status (SRS), number of Striga per plot (NSPlot), and Pod weight (PW) while low heritability estimates were observed for fodder weight (FW) and Plant Aspect (PASP). Twenty-four SNP markers were identified across SRS, NSPlot, FW, PASP, and PW under Striga-infested conditions. Under non-infested conditions, 17 SNPs were identified across FW, PASP, and PW. Gene annotation of the significant SNPs revealed candidate genes such as ubiquitin ligase activity, cell wall reinforcement protein, and pathogenesis-related protein. These genes function in plant growth regulation, development, and defense mechanisms. This study offers valuable insights for marker selection, validation, and deployment in cowpea improvement for Striga resistance in SSA. |
| format | Journal Article |
| id | CGSpace151959 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1519592025-12-08T10:11:39Z Genome-wide association studies reveals new candidate genes associated with resistance to Striga gesneroides in Cowpea [Vigna unguiculata (L.) Walp.] accessions from sub-Saharan Africa Koura, A.A. Kena, A.W. Annor, B. Adejumobi, I.I. Sayadi Maazou, A.R. Awuku, F.J. Attamah, P. Boukar, O. Akromah, R. cowpeas weeds marker-assisted selection genes single nucleotide polymorphisms striga hermonthica Parasitic plant Striga gesneroides reduces cowpea productivity in sub-Saharan Africa, leading to substantial yield losses. This weed is the major reason for land abandonment by many farmers. While various methods have been proposed for managing Striga invasion and parasitism, host-plant resistance remains the most effective and affordable option for small-scale farmers in the sub-region. This study aims to expedite the improvement of Striga resistance breeding through marker-assisted selection by identifying genomic regions and candidate genes associated with Striga resistance indicator traits using association mapping. A panel of 188 cowpea accessions assembled from five gene banks in West Africa were phenotyped for two years under Striga-infested and non-infested research conditions. These accessions were equally genotyped using the medium-density genotyping of the Diversity Arrays Technology sequencing (DArTseq) platform to obtain marker information. High broad-sense heritability estimates were observed for Striga resistance status (SRS), number of Striga per plot (NSPlot), and Pod weight (PW) while low heritability estimates were observed for fodder weight (FW) and Plant Aspect (PASP). Twenty-four SNP markers were identified across SRS, NSPlot, FW, PASP, and PW under Striga-infested conditions. Under non-infested conditions, 17 SNPs were identified across FW, PASP, and PW. Gene annotation of the significant SNPs revealed candidate genes such as ubiquitin ligase activity, cell wall reinforcement protein, and pathogenesis-related protein. These genes function in plant growth regulation, development, and defense mechanisms. This study offers valuable insights for marker selection, validation, and deployment in cowpea improvement for Striga resistance in SSA. 2024-09 2024-09-03T07:33:18Z 2024-09-03T07:33:18Z Journal Article https://hdl.handle.net/10568/151959 en Limited Access Elsevier Koura, A.A., Kena, A.W., Annor, B., Adejumobi, I.I., Sayadi Maazou, A.R., Awuku, F.J., ... & Akromah, R. (2024). Genome-wide association studies reveals new candidate genes associated with resistance to striga gesneroides in cowpea [Vigna unguiculata (L.) Walp.] Accessions from sub-Saharan Africa. Ecological Genetics and Genomics, 32: 100267, 1-8. |
| spellingShingle | cowpeas weeds marker-assisted selection genes single nucleotide polymorphisms striga hermonthica Koura, A.A. Kena, A.W. Annor, B. Adejumobi, I.I. Sayadi Maazou, A.R. Awuku, F.J. Attamah, P. Boukar, O. Akromah, R. Genome-wide association studies reveals new candidate genes associated with resistance to Striga gesneroides in Cowpea [Vigna unguiculata (L.) Walp.] accessions from sub-Saharan Africa |
| title | Genome-wide association studies reveals new candidate genes associated with resistance to Striga gesneroides in Cowpea [Vigna unguiculata (L.) Walp.] accessions from sub-Saharan Africa |
| title_full | Genome-wide association studies reveals new candidate genes associated with resistance to Striga gesneroides in Cowpea [Vigna unguiculata (L.) Walp.] accessions from sub-Saharan Africa |
| title_fullStr | Genome-wide association studies reveals new candidate genes associated with resistance to Striga gesneroides in Cowpea [Vigna unguiculata (L.) Walp.] accessions from sub-Saharan Africa |
| title_full_unstemmed | Genome-wide association studies reveals new candidate genes associated with resistance to Striga gesneroides in Cowpea [Vigna unguiculata (L.) Walp.] accessions from sub-Saharan Africa |
| title_short | Genome-wide association studies reveals new candidate genes associated with resistance to Striga gesneroides in Cowpea [Vigna unguiculata (L.) Walp.] accessions from sub-Saharan Africa |
| title_sort | genome wide association studies reveals new candidate genes associated with resistance to striga gesneroides in cowpea vigna unguiculata l walp accessions from sub saharan africa |
| topic | cowpeas weeds marker-assisted selection genes single nucleotide polymorphisms striga hermonthica |
| url | https://hdl.handle.net/10568/151959 |
| work_keys_str_mv | AT kouraaa genomewideassociationstudiesrevealsnewcandidategenesassociatedwithresistancetostrigagesneroidesincowpeavignaunguiculatalwalpaccessionsfromsubsaharanafrica AT kenaaw genomewideassociationstudiesrevealsnewcandidategenesassociatedwithresistancetostrigagesneroidesincowpeavignaunguiculatalwalpaccessionsfromsubsaharanafrica AT annorb genomewideassociationstudiesrevealsnewcandidategenesassociatedwithresistancetostrigagesneroidesincowpeavignaunguiculatalwalpaccessionsfromsubsaharanafrica AT adejumobiii genomewideassociationstudiesrevealsnewcandidategenesassociatedwithresistancetostrigagesneroidesincowpeavignaunguiculatalwalpaccessionsfromsubsaharanafrica AT sayadimaazouar genomewideassociationstudiesrevealsnewcandidategenesassociatedwithresistancetostrigagesneroidesincowpeavignaunguiculatalwalpaccessionsfromsubsaharanafrica AT awukufj genomewideassociationstudiesrevealsnewcandidategenesassociatedwithresistancetostrigagesneroidesincowpeavignaunguiculatalwalpaccessionsfromsubsaharanafrica AT attamahp genomewideassociationstudiesrevealsnewcandidategenesassociatedwithresistancetostrigagesneroidesincowpeavignaunguiculatalwalpaccessionsfromsubsaharanafrica AT boukaro genomewideassociationstudiesrevealsnewcandidategenesassociatedwithresistancetostrigagesneroidesincowpeavignaunguiculatalwalpaccessionsfromsubsaharanafrica AT akromahr genomewideassociationstudiesrevealsnewcandidategenesassociatedwithresistancetostrigagesneroidesincowpeavignaunguiculatalwalpaccessionsfromsubsaharanafrica |