A novel Immuno-Polymerase chain reaction protocol incorporating a highly purified Streptavidin-DNA conjugate
We have developed an immuno-polymerase chain reaction protocol which includes a highly purified streptavidin-DNA conjugate. The protocol comprises standard ELISA methodology and washing buffers together with a real-time PCR read-out system. The conjugate was employed in both indirect and capture ass...
| Main Authors: | , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Informa UK Limited
2009
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| Online Access: | https://hdl.handle.net/10568/1494 |
| _version_ | 1855537672050704384 |
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| author | Shan, J. Toye, Philip G. |
| author_browse | Shan, J. Toye, Philip G. |
| author_facet | Shan, J. Toye, Philip G. |
| author_sort | Shan, J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | We have developed an immuno-polymerase chain reaction protocol which includes a highly purified streptavidin-DNA conjugate. The protocol comprises standard ELISA methodology and washing buffers together with a real-time PCR read-out system. The conjugate was employed in both indirect and capture assay formats, which can be completed in a single day using standard laboratory equipment. The immuno-PCR is reproducible, with a larger dynamic range and a sensitivity several orders of magnitude greater than the corresponding conventional ELISA. The minimum concentration of analyte detected was of the order of 1 pg/mL These characteristics should contribute to the adoption of immuno-PCR by research and clinical laboratories. |
| format | Journal Article |
| id | CGSpace1494 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | Informa UK Limited |
| publisherStr | Informa UK Limited |
| record_format | dspace |
| spelling | CGSpace14942024-05-01T08:19:18Z A novel Immuno-Polymerase chain reaction protocol incorporating a highly purified Streptavidin-DNA conjugate Shan, J. Toye, Philip G. We have developed an immuno-polymerase chain reaction protocol which includes a highly purified streptavidin-DNA conjugate. The protocol comprises standard ELISA methodology and washing buffers together with a real-time PCR read-out system. The conjugate was employed in both indirect and capture assay formats, which can be completed in a single day using standard laboratory equipment. The immuno-PCR is reproducible, with a larger dynamic range and a sensitivity several orders of magnitude greater than the corresponding conventional ELISA. The minimum concentration of analyte detected was of the order of 1 pg/mL These characteristics should contribute to the adoption of immuno-PCR by research and clinical laboratories. 2009-06-30 2010-05-11T17:13:53Z 2010-05-11T17:13:53Z Journal Article https://hdl.handle.net/10568/1494 en Limited Access Informa UK Limited Shan, J.; Toye, P.G. 2009. A novel immuno-polymerase chain reaction protocol incorporating a highly purified streptavidin-DNA conjugate. Journal of Immunoassay and Immunochemistry 30:322-337. |
| spellingShingle | Shan, J. Toye, Philip G. A novel Immuno-Polymerase chain reaction protocol incorporating a highly purified Streptavidin-DNA conjugate |
| title | A novel Immuno-Polymerase chain reaction protocol incorporating a highly purified Streptavidin-DNA conjugate |
| title_full | A novel Immuno-Polymerase chain reaction protocol incorporating a highly purified Streptavidin-DNA conjugate |
| title_fullStr | A novel Immuno-Polymerase chain reaction protocol incorporating a highly purified Streptavidin-DNA conjugate |
| title_full_unstemmed | A novel Immuno-Polymerase chain reaction protocol incorporating a highly purified Streptavidin-DNA conjugate |
| title_short | A novel Immuno-Polymerase chain reaction protocol incorporating a highly purified Streptavidin-DNA conjugate |
| title_sort | novel immuno polymerase chain reaction protocol incorporating a highly purified streptavidin dna conjugate |
| url | https://hdl.handle.net/10568/1494 |
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