ACE2 mediates tryptophan alleviation on diarrhea by repairing intestine barrier involved mTOR pathway
The membrane-delimited receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), angiotensin-converting enzyme 2 (ACE2), which is expressed in the intestine, collaborates with broad neutral amino acid transporter 1 (B0AT1). Tryptophan (Trp) is transported into intestinal epithelial...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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BioMed Central
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/149099 |
| _version_ | 1855534812516843520 |
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| author | Jinze Li Yingli Yan Yang Fu Zhe Chen Yongjie Yang Yu Li Jie Pan Feiwu Li Cuifang Zha Kai Miao Lukuyu, Ben A. Kashif Saleemi, M. Yongwen Zhu Hui Ye Lin Yang Wence Wang |
| author_browse | Cuifang Zha Feiwu Li Hui Ye Jie Pan Jinze Li Kai Miao Kashif Saleemi, M. Lin Yang Lukuyu, Ben A. Wence Wang Yang Fu Yingli Yan Yongjie Yang Yongwen Zhu Yu Li Zhe Chen |
| author_facet | Jinze Li Yingli Yan Yang Fu Zhe Chen Yongjie Yang Yu Li Jie Pan Feiwu Li Cuifang Zha Kai Miao Lukuyu, Ben A. Kashif Saleemi, M. Yongwen Zhu Hui Ye Lin Yang Wence Wang |
| author_sort | Jinze Li |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The membrane-delimited receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), angiotensin-converting enzyme 2 (ACE2), which is expressed in the intestine, collaborates with broad neutral amino acid transporter 1 (B0AT1). Tryptophan (Trp) is transported into intestinal epithelial cells by ACE2 and B0AT1. However, whether ACE2 and its binding protein B0AT1 are involved in Trp-mediated alleviation of intestinal injury is largely unknown. Here, we used weaned piglets and IPEC-J2 cells as models and found that ACE2/B0AT1 alleviated lipopolysaccharide (LPS)-induced diarrhea and promoted intestinal barrier recovery via transport of Trp. The levels of the aryl hydrocarbon receptor (AhR) and mechanistic target of rapamycin (mTOR) pathways were altered by ACE2. Dietary Trp supplementation in LPS-treated weaned piglets revealed that Trp alleviated diarrhea by promoting ACE2/B0AT1 expression, and examination of intestinal morphology revealed that the damage to the intestinal barrier was repaired. Our study demonstrated that ACE2 accompanied by B0AT1 mediated the alleviation of diarrhea by Trp through intestinal barrier repair via the mTOR pathway. |
| format | Journal Article |
| id | CGSpace149099 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | BioMed Central |
| publisherStr | BioMed Central |
| record_format | dspace |
| spelling | CGSpace1490992024-11-15T08:52:55Z ACE2 mediates tryptophan alleviation on diarrhea by repairing intestine barrier involved mTOR pathway Jinze Li Yingli Yan Yang Fu Zhe Chen Yongjie Yang Yu Li Jie Pan Feiwu Li Cuifang Zha Kai Miao Lukuyu, Ben A. Kashif Saleemi, M. Yongwen Zhu Hui Ye Lin Yang Wence Wang tryptophan animal health COVID-19 The membrane-delimited receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), angiotensin-converting enzyme 2 (ACE2), which is expressed in the intestine, collaborates with broad neutral amino acid transporter 1 (B0AT1). Tryptophan (Trp) is transported into intestinal epithelial cells by ACE2 and B0AT1. However, whether ACE2 and its binding protein B0AT1 are involved in Trp-mediated alleviation of intestinal injury is largely unknown. Here, we used weaned piglets and IPEC-J2 cells as models and found that ACE2/B0AT1 alleviated lipopolysaccharide (LPS)-induced diarrhea and promoted intestinal barrier recovery via transport of Trp. The levels of the aryl hydrocarbon receptor (AhR) and mechanistic target of rapamycin (mTOR) pathways were altered by ACE2. Dietary Trp supplementation in LPS-treated weaned piglets revealed that Trp alleviated diarrhea by promoting ACE2/B0AT1 expression, and examination of intestinal morphology revealed that the damage to the intestinal barrier was repaired. Our study demonstrated that ACE2 accompanied by B0AT1 mediated the alleviation of diarrhea by Trp through intestinal barrier repair via the mTOR pathway. 2024 2024-07-16T06:28:57Z 2024-07-16T06:28:57Z Journal Article https://hdl.handle.net/10568/149099 en Open Access BioMed Central Jinze Li, Yingli Yan, Yang Fu, Zhe Chen, Yongjie Yang, Yu Li, Jie Pan, Feiwu Li, Cuifang Zha, Kai Miao, Lukuyu, B., Kashif Saleemi, M., Yongwen Zhu, Hui Ye, Lin Yang and Wence Wang. 2024. ACE2 mediates tryptophan alleviation on diarrhea by repairing intestine barrier involved mTOR pathway. Cellular & Molecular Biology Letters 29:90. |
| spellingShingle | tryptophan animal health COVID-19 Jinze Li Yingli Yan Yang Fu Zhe Chen Yongjie Yang Yu Li Jie Pan Feiwu Li Cuifang Zha Kai Miao Lukuyu, Ben A. Kashif Saleemi, M. Yongwen Zhu Hui Ye Lin Yang Wence Wang ACE2 mediates tryptophan alleviation on diarrhea by repairing intestine barrier involved mTOR pathway |
| title | ACE2 mediates tryptophan alleviation on diarrhea by repairing intestine barrier involved mTOR pathway |
| title_full | ACE2 mediates tryptophan alleviation on diarrhea by repairing intestine barrier involved mTOR pathway |
| title_fullStr | ACE2 mediates tryptophan alleviation on diarrhea by repairing intestine barrier involved mTOR pathway |
| title_full_unstemmed | ACE2 mediates tryptophan alleviation on diarrhea by repairing intestine barrier involved mTOR pathway |
| title_short | ACE2 mediates tryptophan alleviation on diarrhea by repairing intestine barrier involved mTOR pathway |
| title_sort | ace2 mediates tryptophan alleviation on diarrhea by repairing intestine barrier involved mtor pathway |
| topic | tryptophan animal health COVID-19 |
| url | https://hdl.handle.net/10568/149099 |
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