High prevalence of highly pathogenic avian influenza A virus in Vietnam’s live bird markets

Background: In recent years Vietnam has suffered multiple epizootics of influenza in poultry. Methods: From January 10th, 2019 to April 26th, 2021, we employed a One Health influenza surveillance approach at live bird markets (LBMs) and swine farms in Northern Vietnam. When the COVID-19 pandemic pe...

Full description

Bibliographic Details
Main Authors: Duy Tung Dao, Coleman, K.K., Vuong N. Bui, Anh N. Bui, Long H. Tran, Quy D. Nguyen, Son Than, Pulscher, L.A., Marushchak, L.V., Robie, E.R., Hung Nguyen-Viet, Phuc Duc Pham, Christy, N.C., Brooks, J.S., Huy C. Nguyen, Rubrum, A.M., Webby, R.J., Gray, G.C.
Format: Journal Article
Language:Inglés
Published: Oxford University Press 2024
Subjects:
Online Access:https://hdl.handle.net/10568/149074
_version_ 1855532728526569472
author Duy Tung Dao
Coleman, K.K.
Vuong N. Bui
Anh N. Bui
Long H. Tran
Quy D. Nguyen
Son Than
Pulscher, L.A.
Marushchak, L.V.
Robie, E.R.
Hung Nguyen-Viet
Phuc Duc Pham
Christy, N.C.
Brooks, J.S.
Huy C. Nguyen
Rubrum, A.M.
Webby, R.J.
Gray, G.C.
author_browse Anh N. Bui
Brooks, J.S.
Christy, N.C.
Coleman, K.K.
Duy Tung Dao
Gray, G.C.
Hung Nguyen-Viet
Huy C. Nguyen
Long H. Tran
Marushchak, L.V.
Phuc Duc Pham
Pulscher, L.A.
Quy D. Nguyen
Robie, E.R.
Rubrum, A.M.
Son Than
Vuong N. Bui
Webby, R.J.
author_facet Duy Tung Dao
Coleman, K.K.
Vuong N. Bui
Anh N. Bui
Long H. Tran
Quy D. Nguyen
Son Than
Pulscher, L.A.
Marushchak, L.V.
Robie, E.R.
Hung Nguyen-Viet
Phuc Duc Pham
Christy, N.C.
Brooks, J.S.
Huy C. Nguyen
Rubrum, A.M.
Webby, R.J.
Gray, G.C.
author_sort Duy Tung Dao
collection Repository of Agricultural Research Outputs (CGSpace)
description Background: In recent years Vietnam has suffered multiple epizootics of influenza in poultry. Methods: From January 10th, 2019 to April 26th, 2021, we employed a One Health influenza surveillance approach at live bird markets (LBMs) and swine farms in Northern Vietnam. When the COVID-19 pandemic permitted, each month field teams collected oral secretion samples from poultry and pigs, animal facility bioaerosol and fecal samples, and animal worker nasal washes at four LBMs and five swine farms across five sites. Initially samples were screened with molecular assays followed by culture in embryonated eggs (poultry swabs) or MDCK cells (human or swine swabs). Results: Many of the 3,493 samples collected had either molecular or culture evidence for influenza A virus including 314 (37.5%) of the 837 poultry oropharyngeal swabs, 144 (25.1%) of the 574 bioaerosol samples, 438 (34.9 %) of the 1,257 poultry fecal swab samples and 16 (1.9%) of the 828 human nasal washes. Culturing poultry samples yielded 454 influenza A isolates, 83 of which were H5 and 70 (84.3%) of these were highly pathogenic. Additionally, a positive human sample had a H9N2 avian-like PB1 gene. In contrast, the prevalence of influenza A in the swine farms was much lower with only 6 (0.4%) of the 1,700 total swine farm samples studied, having molecular evidence for influenza A virus. Conclusions: This study suggests that Vietnam’s LBMs continue to harbor high prevalences of avian influenza A viruses, including many highly pathogenic H5N6 strains, which will continue to threaten poultry and humans.
format Journal Article
id CGSpace149074
institution CGIAR Consortium
language Inglés
publishDate 2024
publishDateRange 2024
publishDateSort 2024
publisher Oxford University Press
publisherStr Oxford University Press
record_format dspace
spelling CGSpace1490742025-12-08T10:06:44Z High prevalence of highly pathogenic avian influenza A virus in Vietnam’s live bird markets Duy Tung Dao Coleman, K.K. Vuong N. Bui Anh N. Bui Long H. Tran Quy D. Nguyen Son Than Pulscher, L.A. Marushchak, L.V. Robie, E.R. Hung Nguyen-Viet Phuc Duc Pham Christy, N.C. Brooks, J.S. Huy C. Nguyen Rubrum, A.M. Webby, R.J. Gray, G.C. avian influenza poultry zoonoses Background: In recent years Vietnam has suffered multiple epizootics of influenza in poultry. Methods: From January 10th, 2019 to April 26th, 2021, we employed a One Health influenza surveillance approach at live bird markets (LBMs) and swine farms in Northern Vietnam. When the COVID-19 pandemic permitted, each month field teams collected oral secretion samples from poultry and pigs, animal facility bioaerosol and fecal samples, and animal worker nasal washes at four LBMs and five swine farms across five sites. Initially samples were screened with molecular assays followed by culture in embryonated eggs (poultry swabs) or MDCK cells (human or swine swabs). Results: Many of the 3,493 samples collected had either molecular or culture evidence for influenza A virus including 314 (37.5%) of the 837 poultry oropharyngeal swabs, 144 (25.1%) of the 574 bioaerosol samples, 438 (34.9 %) of the 1,257 poultry fecal swab samples and 16 (1.9%) of the 828 human nasal washes. Culturing poultry samples yielded 454 influenza A isolates, 83 of which were H5 and 70 (84.3%) of these were highly pathogenic. Additionally, a positive human sample had a H9N2 avian-like PB1 gene. In contrast, the prevalence of influenza A in the swine farms was much lower with only 6 (0.4%) of the 1,700 total swine farm samples studied, having molecular evidence for influenza A virus. Conclusions: This study suggests that Vietnam’s LBMs continue to harbor high prevalences of avian influenza A viruses, including many highly pathogenic H5N6 strains, which will continue to threaten poultry and humans. 2024-06-28 2024-07-15T08:07:04Z 2024-07-15T08:07:04Z Journal Article https://hdl.handle.net/10568/149074 en Open Access Oxford University Press Duy Tung Dao, Coleman, K.K., Vuong N. Bui, Anh N. Bui, Long H. Tran, Quy D. Nguyen, Son Than, Pulscher, L.A., Marushchak, L.V., Robie, E.R., Hung Nguyen-Viet, Phuc Duc Pham, Christy, N.C., Brooks, J.S., Huy C. Nguyen, Rubrum, A.M., Webby, R.J., and Gray, G.C. 2024. High prevalence of highly pathogenic avian influenza A virus in Vietnam’s live bird markets. Open Forum Infectious Diseases 11(7): ofae355.
spellingShingle avian influenza
poultry
zoonoses
Duy Tung Dao
Coleman, K.K.
Vuong N. Bui
Anh N. Bui
Long H. Tran
Quy D. Nguyen
Son Than
Pulscher, L.A.
Marushchak, L.V.
Robie, E.R.
Hung Nguyen-Viet
Phuc Duc Pham
Christy, N.C.
Brooks, J.S.
Huy C. Nguyen
Rubrum, A.M.
Webby, R.J.
Gray, G.C.
High prevalence of highly pathogenic avian influenza A virus in Vietnam’s live bird markets
title High prevalence of highly pathogenic avian influenza A virus in Vietnam’s live bird markets
title_full High prevalence of highly pathogenic avian influenza A virus in Vietnam’s live bird markets
title_fullStr High prevalence of highly pathogenic avian influenza A virus in Vietnam’s live bird markets
title_full_unstemmed High prevalence of highly pathogenic avian influenza A virus in Vietnam’s live bird markets
title_short High prevalence of highly pathogenic avian influenza A virus in Vietnam’s live bird markets
title_sort high prevalence of highly pathogenic avian influenza a virus in vietnam s live bird markets
topic avian influenza
poultry
zoonoses
url https://hdl.handle.net/10568/149074
work_keys_str_mv AT duytungdao highprevalenceofhighlypathogenicavianinfluenzaavirusinvietnamslivebirdmarkets
AT colemankk highprevalenceofhighlypathogenicavianinfluenzaavirusinvietnamslivebirdmarkets
AT vuongnbui highprevalenceofhighlypathogenicavianinfluenzaavirusinvietnamslivebirdmarkets
AT anhnbui highprevalenceofhighlypathogenicavianinfluenzaavirusinvietnamslivebirdmarkets
AT longhtran highprevalenceofhighlypathogenicavianinfluenzaavirusinvietnamslivebirdmarkets
AT quydnguyen highprevalenceofhighlypathogenicavianinfluenzaavirusinvietnamslivebirdmarkets
AT sonthan highprevalenceofhighlypathogenicavianinfluenzaavirusinvietnamslivebirdmarkets
AT pulscherla highprevalenceofhighlypathogenicavianinfluenzaavirusinvietnamslivebirdmarkets
AT marushchaklv highprevalenceofhighlypathogenicavianinfluenzaavirusinvietnamslivebirdmarkets
AT robieer highprevalenceofhighlypathogenicavianinfluenzaavirusinvietnamslivebirdmarkets
AT hungnguyenviet highprevalenceofhighlypathogenicavianinfluenzaavirusinvietnamslivebirdmarkets
AT phucducpham highprevalenceofhighlypathogenicavianinfluenzaavirusinvietnamslivebirdmarkets
AT christync highprevalenceofhighlypathogenicavianinfluenzaavirusinvietnamslivebirdmarkets
AT brooksjs highprevalenceofhighlypathogenicavianinfluenzaavirusinvietnamslivebirdmarkets
AT huycnguyen highprevalenceofhighlypathogenicavianinfluenzaavirusinvietnamslivebirdmarkets
AT rubrumam highprevalenceofhighlypathogenicavianinfluenzaavirusinvietnamslivebirdmarkets
AT webbyrj highprevalenceofhighlypathogenicavianinfluenzaavirusinvietnamslivebirdmarkets
AT graygc highprevalenceofhighlypathogenicavianinfluenzaavirusinvietnamslivebirdmarkets