Association mapping of septoria tritici blotch resistance in bread wheat in Bale and Arsi highlands, Ethiopia

Septoria tritici blotch (STB) caused by the fungal pathogen Zymoseptoria tritici, anamorph Septoria tritici Rob. ex Desm., is an important wheat pathogen worldwide, reported to be major wheat production threating factor, posing considerable yield loss every year. Developing resistant cultivars is an...

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Main Authors: Binalf, Lakachew, Shifa, Hassen, Tadesse, Wuletaw
Format: Journal Article
Language:Inglés
Published: Elsevier 2024
Subjects:
Online Access:https://hdl.handle.net/10568/145223
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author Binalf, Lakachew
Shifa, Hassen
Tadesse, Wuletaw
author_browse Binalf, Lakachew
Shifa, Hassen
Tadesse, Wuletaw
author_facet Binalf, Lakachew
Shifa, Hassen
Tadesse, Wuletaw
author_sort Binalf, Lakachew
collection Repository of Agricultural Research Outputs (CGSpace)
description Septoria tritici blotch (STB) caused by the fungal pathogen Zymoseptoria tritici, anamorph Septoria tritici Rob. ex Desm., is an important wheat pathogen worldwide, reported to be major wheat production threating factor, posing considerable yield loss every year. Developing resistant cultivars is an efficient, economical, environmentally friendly and simple approach for managing STB. This study was carried out to evaluate spring bread wheat lines for their reaction to STB disease under field conditions; to associate phenotypic and genotypic data for identification of STB disease resistance; and to identify genomic region(s) associated with resistance to STB in spring bread wheat lines. Two hundred forty (240) spring bread wheat lines were evaluated under field conditions in non-replicated trials, using an augmented design. The trials were conducted at three locations (Kulumsa Agricultural Research Center, Madda Walabu University Research Site and Sinana Agricultural Research Center) in 2017 main cropping season (July to December). Out of these 240 wheat lines, 123 of them were genotyped with 10263 single nucleotide polymorphism (SNPs) markers and population structure and association mapping analysis was done. The wheat lines showed significant variations in percentage disease severity and area under the disease progress curve at all the three locations they were evaluated. The wheat lines were classified as resistant, moderately resistant, moderately susceptible and susceptible based on the percentage disease severity scored. Five wheat lines were found to be resistant to STB in all the three locations and are recommended for direct release by the national program and parentage purposes in wheat breeding programs. The 123 wheat lines were clustered into 3 subpopulations in which the first cluster contained 99 wheat lines; the second 17 and the last one 7. Among the polymorphic 8127 SNPs markers, 26 markers on chromosomes 7B, 1D, 3A, 2B, 6B and 3D were found to be significantly (P < 0.001) associated with STB resistance so that they can be utilized for marker assisted selection and gene pyramiding in resistance breeding programs.
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spelling CGSpace1452232026-01-15T02:03:47Z Association mapping of septoria tritici blotch resistance in bread wheat in Bale and Arsi highlands, Ethiopia Binalf, Lakachew Shifa, Hassen Tadesse, Wuletaw bread wheat association mapping soft wheat resistance septoria tritici blotch percentage disease severity area under disease progress curve Septoria tritici blotch (STB) caused by the fungal pathogen Zymoseptoria tritici, anamorph Septoria tritici Rob. ex Desm., is an important wheat pathogen worldwide, reported to be major wheat production threating factor, posing considerable yield loss every year. Developing resistant cultivars is an efficient, economical, environmentally friendly and simple approach for managing STB. This study was carried out to evaluate spring bread wheat lines for their reaction to STB disease under field conditions; to associate phenotypic and genotypic data for identification of STB disease resistance; and to identify genomic region(s) associated with resistance to STB in spring bread wheat lines. Two hundred forty (240) spring bread wheat lines were evaluated under field conditions in non-replicated trials, using an augmented design. The trials were conducted at three locations (Kulumsa Agricultural Research Center, Madda Walabu University Research Site and Sinana Agricultural Research Center) in 2017 main cropping season (July to December). Out of these 240 wheat lines, 123 of them were genotyped with 10263 single nucleotide polymorphism (SNPs) markers and population structure and association mapping analysis was done. The wheat lines showed significant variations in percentage disease severity and area under the disease progress curve at all the three locations they were evaluated. The wheat lines were classified as resistant, moderately resistant, moderately susceptible and susceptible based on the percentage disease severity scored. Five wheat lines were found to be resistant to STB in all the three locations and are recommended for direct release by the national program and parentage purposes in wheat breeding programs. The 123 wheat lines were clustered into 3 subpopulations in which the first cluster contained 99 wheat lines; the second 17 and the last one 7. Among the polymorphic 8127 SNPs markers, 26 markers on chromosomes 7B, 1D, 3A, 2B, 6B and 3D were found to be significantly (P < 0.001) associated with STB resistance so that they can be utilized for marker assisted selection and gene pyramiding in resistance breeding programs. 2024-06-13T20:02:48Z 2024-06-13T20:02:48Z Journal Article https://hdl.handle.net/10568/145223 en Open Access application/pdf Elsevier Lakachew Binalf, Hassen Shifa, Wuletaw Tadesse. (31/5/2024). Association mapping of septoria tritici blotch resistance in bread wheat in Bale and Arsi highlands, Ethiopia. Heliyon, 10 (11).
spellingShingle bread wheat
association mapping
soft wheat
resistance
septoria tritici blotch
percentage disease severity
area under disease progress curve
Binalf, Lakachew
Shifa, Hassen
Tadesse, Wuletaw
Association mapping of septoria tritici blotch resistance in bread wheat in Bale and Arsi highlands, Ethiopia
title Association mapping of septoria tritici blotch resistance in bread wheat in Bale and Arsi highlands, Ethiopia
title_full Association mapping of septoria tritici blotch resistance in bread wheat in Bale and Arsi highlands, Ethiopia
title_fullStr Association mapping of septoria tritici blotch resistance in bread wheat in Bale and Arsi highlands, Ethiopia
title_full_unstemmed Association mapping of septoria tritici blotch resistance in bread wheat in Bale and Arsi highlands, Ethiopia
title_short Association mapping of septoria tritici blotch resistance in bread wheat in Bale and Arsi highlands, Ethiopia
title_sort association mapping of septoria tritici blotch resistance in bread wheat in bale and arsi highlands ethiopia
topic bread wheat
association mapping
soft wheat
resistance
septoria tritici blotch
percentage disease severity
area under disease progress curve
url https://hdl.handle.net/10568/145223
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