Tuber transcriptome analysis reveals a novel WRKY transcription factor StWRKY70 potentially involved in potato pigmentation
Tuber flesh pigmentation, conferred by the presence of secondary metabolite anthocyanins, is one of many key agronomic traits for potato tubers. Although several genes of potato anthocyanin biosynthesis have been reported, transcription factors (TFs) contributing to tuber flesh pigmentation are stil...
| Autores principales: | , , , , , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2024
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/145136 |
| _version_ | 1855534450015731712 |
|---|---|
| author | Zhang, Y. Pu, Y. Li, K. Bai, S. Wang, J. Xu, M. Liu, S. Zhou, Z. Wu, Y. Hu, R. Wu, Q. Kear, P. Du, M. Qi, J. |
| author_browse | Bai, S. Du, M. Hu, R. Kear, P. Li, K. Liu, S. Pu, Y. Qi, J. Wang, J. Wu, Q. Wu, Y. Xu, M. Zhang, Y. Zhou, Z. |
| author_facet | Zhang, Y. Pu, Y. Li, K. Bai, S. Wang, J. Xu, M. Liu, S. Zhou, Z. Wu, Y. Hu, R. Wu, Q. Kear, P. Du, M. Qi, J. |
| author_sort | Zhang, Y. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Tuber flesh pigmentation, conferred by the presence of secondary metabolite anthocyanins, is one of many key agronomic traits for potato tubers. Although several genes of potato anthocyanin biosynthesis have been reported, transcription factors (TFs) contributing to tuber flesh pigmentation are still not fully understood. In this study, transcriptomic profiling of diploid potato accessions with or without tuber flesh pigmentation was conducted and genes of the anthocyanin biosynthesis pathway were found significantly enriched within the 1435 differentially expressed genes (DEGs). Weighted Gene Co-expression Network Analysis (WGCNA) and connectivity analysis pinpointed a subset of 173 genes closely related to the key biosynthetic gene StDFR. Of the eight transcription factors in the subset, group III WRKY StWRKY70, was chosen for showing high connectivity to StDFR and ten other anthocyanin biosynthetic genes and homology to known WRKYs of anthocyanin pathway. The transient activation assay showed StWRKY70 predominantly stimulated the expression of StDFR and StANS as well as the accumulation of anthocyanins by enhancing the function of the MYB transcription factor StAN1. Furthermore, the interaction between StWRKY70 and StAN1 was verified by Y2H and BiFC. Our analysis discovered a new transcriptional activator StWRKY70 which potentially involved in tuber flesh pigmentation, thus may lay the foundation for deciphering how the WRKY-MYB-bHLH-WD40 (WRKY-MBW) complex regulate the accumulation of anthocyanins and provide new strategies to breed for more nutritious potato varieties with enhanced tuber flesh anthocyanins. |
| format | Journal Article |
| id | CGSpace145136 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1451362025-10-26T13:02:19Z Tuber transcriptome analysis reveals a novel WRKY transcription factor StWRKY70 potentially involved in potato pigmentation Zhang, Y. Pu, Y. Li, K. Bai, S. Wang, J. Xu, M. Liu, S. Zhou, Z. Wu, Y. Hu, R. Wu, Q. Kear, P. Du, M. Qi, J. anthocyanins potatoes transcriptomics breeding genetics gene expression genomics Tuber flesh pigmentation, conferred by the presence of secondary metabolite anthocyanins, is one of many key agronomic traits for potato tubers. Although several genes of potato anthocyanin biosynthesis have been reported, transcription factors (TFs) contributing to tuber flesh pigmentation are still not fully understood. In this study, transcriptomic profiling of diploid potato accessions with or without tuber flesh pigmentation was conducted and genes of the anthocyanin biosynthesis pathway were found significantly enriched within the 1435 differentially expressed genes (DEGs). Weighted Gene Co-expression Network Analysis (WGCNA) and connectivity analysis pinpointed a subset of 173 genes closely related to the key biosynthetic gene StDFR. Of the eight transcription factors in the subset, group III WRKY StWRKY70, was chosen for showing high connectivity to StDFR and ten other anthocyanin biosynthetic genes and homology to known WRKYs of anthocyanin pathway. The transient activation assay showed StWRKY70 predominantly stimulated the expression of StDFR and StANS as well as the accumulation of anthocyanins by enhancing the function of the MYB transcription factor StAN1. Furthermore, the interaction between StWRKY70 and StAN1 was verified by Y2H and BiFC. Our analysis discovered a new transcriptional activator StWRKY70 which potentially involved in tuber flesh pigmentation, thus may lay the foundation for deciphering how the WRKY-MYB-bHLH-WD40 (WRKY-MBW) complex regulate the accumulation of anthocyanins and provide new strategies to breed for more nutritious potato varieties with enhanced tuber flesh anthocyanins. 2024-08 2024-06-11T15:13:12Z 2024-06-11T15:13:12Z Journal Article https://hdl.handle.net/10568/145136 en Limited Access Elsevier Zhang, Y.; Pu, Y.; Zhang, Y.; Li, K.; Bai, S.; Wang, J.; Xu, M.; Liu, S.; Zhou, Z.; Wu, Y.; Hu, R.; Wu, Q.; Kear, P. Du, M.; Qi, J. 2024. Tuber transcriptome analysis reveals a novel WRKY transcription factor StWRKY70 potentially involved in potato pigmentation. Plant Physiology and Biochemistry. ISSN 1873-2690. 213, 108792. https://doi.org/10.1016/j.plaphy.2024.108792 |
| spellingShingle | anthocyanins potatoes transcriptomics breeding genetics gene expression genomics Zhang, Y. Pu, Y. Li, K. Bai, S. Wang, J. Xu, M. Liu, S. Zhou, Z. Wu, Y. Hu, R. Wu, Q. Kear, P. Du, M. Qi, J. Tuber transcriptome analysis reveals a novel WRKY transcription factor StWRKY70 potentially involved in potato pigmentation |
| title | Tuber transcriptome analysis reveals a novel WRKY transcription factor StWRKY70 potentially involved in potato pigmentation |
| title_full | Tuber transcriptome analysis reveals a novel WRKY transcription factor StWRKY70 potentially involved in potato pigmentation |
| title_fullStr | Tuber transcriptome analysis reveals a novel WRKY transcription factor StWRKY70 potentially involved in potato pigmentation |
| title_full_unstemmed | Tuber transcriptome analysis reveals a novel WRKY transcription factor StWRKY70 potentially involved in potato pigmentation |
| title_short | Tuber transcriptome analysis reveals a novel WRKY transcription factor StWRKY70 potentially involved in potato pigmentation |
| title_sort | tuber transcriptome analysis reveals a novel wrky transcription factor stwrky70 potentially involved in potato pigmentation |
| topic | anthocyanins potatoes transcriptomics breeding genetics gene expression genomics |
| url | https://hdl.handle.net/10568/145136 |
| work_keys_str_mv | AT zhangy tubertranscriptomeanalysisrevealsanovelwrkytranscriptionfactorstwrky70potentiallyinvolvedinpotatopigmentation AT puy tubertranscriptomeanalysisrevealsanovelwrkytranscriptionfactorstwrky70potentiallyinvolvedinpotatopigmentation AT lik tubertranscriptomeanalysisrevealsanovelwrkytranscriptionfactorstwrky70potentiallyinvolvedinpotatopigmentation AT bais tubertranscriptomeanalysisrevealsanovelwrkytranscriptionfactorstwrky70potentiallyinvolvedinpotatopigmentation AT wangj tubertranscriptomeanalysisrevealsanovelwrkytranscriptionfactorstwrky70potentiallyinvolvedinpotatopigmentation AT xum tubertranscriptomeanalysisrevealsanovelwrkytranscriptionfactorstwrky70potentiallyinvolvedinpotatopigmentation AT lius tubertranscriptomeanalysisrevealsanovelwrkytranscriptionfactorstwrky70potentiallyinvolvedinpotatopigmentation AT zhouz tubertranscriptomeanalysisrevealsanovelwrkytranscriptionfactorstwrky70potentiallyinvolvedinpotatopigmentation AT wuy tubertranscriptomeanalysisrevealsanovelwrkytranscriptionfactorstwrky70potentiallyinvolvedinpotatopigmentation AT hur tubertranscriptomeanalysisrevealsanovelwrkytranscriptionfactorstwrky70potentiallyinvolvedinpotatopigmentation AT wuq tubertranscriptomeanalysisrevealsanovelwrkytranscriptionfactorstwrky70potentiallyinvolvedinpotatopigmentation AT kearp tubertranscriptomeanalysisrevealsanovelwrkytranscriptionfactorstwrky70potentiallyinvolvedinpotatopigmentation AT dum tubertranscriptomeanalysisrevealsanovelwrkytranscriptionfactorstwrky70potentiallyinvolvedinpotatopigmentation AT qij tubertranscriptomeanalysisrevealsanovelwrkytranscriptionfactorstwrky70potentiallyinvolvedinpotatopigmentation |