Improving virus diagnostics
A Loop Mediated isothermal Amplification (LAMP) protocol for Sweet potato feathery mottle virus (SPFMV) was successfully tested in the field in Kakamega, Kenya utilizing a rapid extraction method and partially lyophilized reagents and enabled virus detection on site within 30 minutes. A fourth itera...
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| Formato: | Brief |
| Lenguaje: | Inglés |
| Publicado: |
2016
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| Acceso en línea: | https://hdl.handle.net/10568/144185 |
| Sumario: | A Loop Mediated isothermal Amplification (LAMP) protocol for Sweet potato feathery mottle virus (SPFMV) was successfully tested in the field in Kakamega, Kenya utilizing a rapid extraction method and partially lyophilized reagents and enabled virus detection on site within 30 minutes. A fourth iteration of another diagnostic tool, ClonDiag arrays, was successfully tested at the Kenya Plant Health Inspectorate Service (KEPHIS) and a validation plan set up. |
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