Antimicrobial use and antimicrobial resistance in Escherichia coli in semi-intensive and free-range poultry farms in Uganda
Livestock associated antimicrobial resistance (AMR) can reduce productivity and cause economic losses, threatening the livelihoods of poor farming communities in low-income settings. We investigated the practices and risk factors for increased antibiotic use, and AMR in <i>Escherichia coli</i> inclu...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2024
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/144045 |
| _version_ | 1855515054892384256 |
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| author | Mbatidde, Irene Ndoboli, Dickson Ayebare, Dreck Muloi, Dishon Roesel, Kristina Ochieng, Linnet Dione, Michel M. Tenhagen, B.-A. Biryomumaisho, S. Wampande, E. Wieland, Barbara Olsen, J.E. Moodley, Arshnee |
| author_browse | Ayebare, Dreck Biryomumaisho, S. Dione, Michel M. Mbatidde, Irene Moodley, Arshnee Muloi, Dishon Ndoboli, Dickson Ochieng, Linnet Olsen, J.E. Roesel, Kristina Tenhagen, B.-A. Wampande, E. Wieland, Barbara |
| author_facet | Mbatidde, Irene Ndoboli, Dickson Ayebare, Dreck Muloi, Dishon Roesel, Kristina Ochieng, Linnet Dione, Michel M. Tenhagen, B.-A. Biryomumaisho, S. Wampande, E. Wieland, Barbara Olsen, J.E. Moodley, Arshnee |
| author_sort | Mbatidde, Irene |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Livestock associated antimicrobial resistance (AMR) can reduce productivity and cause economic losses, threatening the livelihoods of poor farming communities in low-income settings. We investigated the practices and risk factors for increased antibiotic use, and AMR in <i>Escherichia coli</i> including resistance to human critically important antibiotics like cefotaxime and colistin in semi-intensive and free-range poultry farms in Uganda. Samples and farm management data were collected from 402 poultry farms in two districts between October 2021 to March 2022. Samples were processed to isolate <i>E. coli</i> and to quantify cefotaxime (CTX) and colistin (COL) resistant coliforms. The identification of presumptive <i>E. coli</i> isolated on MacConkey agar without antibiotics, was confirmed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and subjected to antimicrobial susceptibility testing by disk diffusion using EUCAST guidelines. Our models indicated that antibiotic use was associated with production intensity, and type of feed used. Moreover, semi-intensive farmers had better knowledge on antibiotic use compared to farmers in the free-range system. In semi-intensive farms, 52% harbored COL<sup>R</sup> and 57% CTX<sup>R</sup> coliforms. In free-range farms, 54% had COL<sup>R</sup> and 67% CTX<sup>R</sup> coliforms. Resistance to tetracycline, ampicillin and enrofloxacin were more frequent in semi-intensive farms compared to the free-range farms. Multi-drug resistant <i>E. coli</i> were identified in both poultry production systems despite different management and antibiotic use practices. There was no significant relationship between antibiotic use and resistance for the six antibiotics tested. |
| format | Journal Article |
| id | CGSpace144045 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1440452025-10-26T12:54:25Z Antimicrobial use and antimicrobial resistance in Escherichia coli in semi-intensive and free-range poultry farms in Uganda Mbatidde, Irene Ndoboli, Dickson Ayebare, Dreck Muloi, Dishon Roesel, Kristina Ochieng, Linnet Dione, Michel M. Tenhagen, B.-A. Biryomumaisho, S. Wampande, E. Wieland, Barbara Olsen, J.E. Moodley, Arshnee antimicrobial resistance poultry Livestock associated antimicrobial resistance (AMR) can reduce productivity and cause economic losses, threatening the livelihoods of poor farming communities in low-income settings. We investigated the practices and risk factors for increased antibiotic use, and AMR in <i>Escherichia coli</i> including resistance to human critically important antibiotics like cefotaxime and colistin in semi-intensive and free-range poultry farms in Uganda. Samples and farm management data were collected from 402 poultry farms in two districts between October 2021 to March 2022. Samples were processed to isolate <i>E. coli</i> and to quantify cefotaxime (CTX) and colistin (COL) resistant coliforms. The identification of presumptive <i>E. coli</i> isolated on MacConkey agar without antibiotics, was confirmed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and subjected to antimicrobial susceptibility testing by disk diffusion using EUCAST guidelines. Our models indicated that antibiotic use was associated with production intensity, and type of feed used. Moreover, semi-intensive farmers had better knowledge on antibiotic use compared to farmers in the free-range system. In semi-intensive farms, 52% harbored COL<sup>R</sup> and 57% CTX<sup>R</sup> coliforms. In free-range farms, 54% had COL<sup>R</sup> and 67% CTX<sup>R</sup> coliforms. Resistance to tetracycline, ampicillin and enrofloxacin were more frequent in semi-intensive farms compared to the free-range farms. Multi-drug resistant <i>E. coli</i> were identified in both poultry production systems despite different management and antibiotic use practices. There was no significant relationship between antibiotic use and resistance for the six antibiotics tested. 2024-06 2024-05-24T12:09:52Z 2024-05-24T12:09:52Z Journal Article https://hdl.handle.net/10568/144045 en Open Access Elsevier Mbatidde, I., Ndoboli, D., Ayebare, D., Muloi, D., Roesel, K., Ochieng, L., Dione, M., Tenhagen, B.-A., Biryomumaisho, S., Wampande, E., Wieland, B., Olsen, J.E. and Moodley, A. 2024. Antimicrobial use and antimicrobial resistance in <i>Escherichia coli</i> in semi-intensive and free-range poultry farms in Uganda. <i>One Health</i> 18: 100762. |
| spellingShingle | antimicrobial resistance poultry Mbatidde, Irene Ndoboli, Dickson Ayebare, Dreck Muloi, Dishon Roesel, Kristina Ochieng, Linnet Dione, Michel M. Tenhagen, B.-A. Biryomumaisho, S. Wampande, E. Wieland, Barbara Olsen, J.E. Moodley, Arshnee Antimicrobial use and antimicrobial resistance in Escherichia coli in semi-intensive and free-range poultry farms in Uganda |
| title | Antimicrobial use and antimicrobial resistance in Escherichia coli in semi-intensive and free-range poultry farms in Uganda |
| title_full | Antimicrobial use and antimicrobial resistance in Escherichia coli in semi-intensive and free-range poultry farms in Uganda |
| title_fullStr | Antimicrobial use and antimicrobial resistance in Escherichia coli in semi-intensive and free-range poultry farms in Uganda |
| title_full_unstemmed | Antimicrobial use and antimicrobial resistance in Escherichia coli in semi-intensive and free-range poultry farms in Uganda |
| title_short | Antimicrobial use and antimicrobial resistance in Escherichia coli in semi-intensive and free-range poultry farms in Uganda |
| title_sort | antimicrobial use and antimicrobial resistance in escherichia coli in semi intensive and free range poultry farms in uganda |
| topic | antimicrobial resistance poultry |
| url | https://hdl.handle.net/10568/144045 |
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