Detection and antibiogram profile of diarrheagenic Escherichia coli isolated from two abattoir settings in northwest Ethiopia: a one health perspective
Background: Diarrheagenic <i>Escherichia coli</i> (<i>E. coli</i>) is a zoonotic pathogen that contaminates abattoir workers, slaughter environments, slaughter equipment, and carcasses during abattoir processing. Infection with <i>E. coli</i> is associated with the consumption of contaminated food a...
| Autores principales: | , , , , , , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2024
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/141786 |
| _version_ | 1855522202300973056 |
|---|---|
| author | Abey, S.L. Teka, M. Bitew, A.B. Molla, W. Ejo, M. Dagnaw, G.G. Adugna, T. Nigatu, S. Mengistu, B.A. Kinde, M.Z. Berju, A. Belete, M.A. Temesgen, Wudu Dagnachew, S. Tesema, T.S. |
| author_browse | Abey, S.L. Adugna, T. Belete, M.A. Berju, A. Bitew, A.B. Dagnachew, S. Dagnaw, G.G. Ejo, M. Kinde, M.Z. Mengistu, B.A. Molla, W. Nigatu, S. Teka, M. Temesgen, Wudu Tesema, T.S. |
| author_facet | Abey, S.L. Teka, M. Bitew, A.B. Molla, W. Ejo, M. Dagnaw, G.G. Adugna, T. Nigatu, S. Mengistu, B.A. Kinde, M.Z. Berju, A. Belete, M.A. Temesgen, Wudu Dagnachew, S. Tesema, T.S. |
| author_sort | Abey, S.L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Background: Diarrheagenic <i>Escherichia coli</i> (<i>E. coli</i>) is a zoonotic pathogen that contaminates abattoir workers, slaughter environments, slaughter equipment, and carcasses during abattoir processing. Infection with <i>E. coli</i> is associated with the consumption of contaminated food and water, and it is a potential threat to the health and welfare of both humans and animals. Hence, this study aimed to detect diarrheagenic <i>E. coli</i> and assess its antibiogram profile in two abattoir settings, in one health lens.
Methods: A cross-sectional study in one health approach was conducted from December 2020 to June 2021. A total of 384 samples from abattoir workers’ hands, carcasses, knives, cattle feces, abattoir water and effluents were collected. Bacterial culture and biochemical tests were conducted to isolate <i>E. coli</i>, while conventional polymerase chain reaction was performed to identify virulence genes. The antibiogram of diarrheagenic <i>E. coli</i> was tested against nine antimicrobials using the Kirby Bauer disk diffusion method.
Results: A total of 115 (29.95%) <i>E. coli</i> were isolated from the 384 samples, and from these isolates, about 17 (14.8%) were confirmed to be diarrheagenic <i>E. coli</i> (DEC). Among the DEC pathotypes, nine (52.94%), five (29.4%), and three (17.65%) were Shiga toxin-producing, enterohemorrhagic, and enterotoxigenic <i>E. coli</i>, respectively. While 14 (82.35%) DEC isolates harbored the <i>stx2</i>gene, five (29.41%) the <i>eae</i> gene, five (29.41%) the <i>hlyA</i> gene and three (17.65%) harbored the <i>st</i> gene. All the DEC isolates were resistant to erythromycin and vancomycin; whereas, they were susceptible to ampicillin, nalidixic acid and norfloxacin. Furthermore, 64.7% of DEC isolates showed resistance to both ceftazidime and kanamycin and 88.24% of the isolates showed multidrug resistance.
Conclusion: This study detected DEC isolates having different virulence genes, which showed single and multiple antimicrobial resistance. Given the existing poor hygienic and sanitary practices along the abattoir-to-table food chain, coupled with the habit of raw meat consumption, this result indicates a potential public and animal health risk from the pathogen and antimicrobial resistance. |
| format | Journal Article |
| id | CGSpace141786 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1417862025-12-08T10:06:44Z Detection and antibiogram profile of diarrheagenic Escherichia coli isolated from two abattoir settings in northwest Ethiopia: a one health perspective Abey, S.L. Teka, M. Bitew, A.B. Molla, W. Ejo, M. Dagnaw, G.G. Adugna, T. Nigatu, S. Mengistu, B.A. Kinde, M.Z. Berju, A. Belete, M.A. Temesgen, Wudu Dagnachew, S. Tesema, T.S. antimicrobial resistance one health approach Background: Diarrheagenic <i>Escherichia coli</i> (<i>E. coli</i>) is a zoonotic pathogen that contaminates abattoir workers, slaughter environments, slaughter equipment, and carcasses during abattoir processing. Infection with <i>E. coli</i> is associated with the consumption of contaminated food and water, and it is a potential threat to the health and welfare of both humans and animals. Hence, this study aimed to detect diarrheagenic <i>E. coli</i> and assess its antibiogram profile in two abattoir settings, in one health lens. Methods: A cross-sectional study in one health approach was conducted from December 2020 to June 2021. A total of 384 samples from abattoir workers’ hands, carcasses, knives, cattle feces, abattoir water and effluents were collected. Bacterial culture and biochemical tests were conducted to isolate <i>E. coli</i>, while conventional polymerase chain reaction was performed to identify virulence genes. The antibiogram of diarrheagenic <i>E. coli</i> was tested against nine antimicrobials using the Kirby Bauer disk diffusion method. Results: A total of 115 (29.95%) <i>E. coli</i> were isolated from the 384 samples, and from these isolates, about 17 (14.8%) were confirmed to be diarrheagenic <i>E. coli</i> (DEC). Among the DEC pathotypes, nine (52.94%), five (29.4%), and three (17.65%) were Shiga toxin-producing, enterohemorrhagic, and enterotoxigenic <i>E. coli</i>, respectively. While 14 (82.35%) DEC isolates harbored the <i>stx2</i>gene, five (29.41%) the <i>eae</i> gene, five (29.41%) the <i>hlyA</i> gene and three (17.65%) harbored the <i>st</i> gene. All the DEC isolates were resistant to erythromycin and vancomycin; whereas, they were susceptible to ampicillin, nalidixic acid and norfloxacin. Furthermore, 64.7% of DEC isolates showed resistance to both ceftazidime and kanamycin and 88.24% of the isolates showed multidrug resistance. Conclusion: This study detected DEC isolates having different virulence genes, which showed single and multiple antimicrobial resistance. Given the existing poor hygienic and sanitary practices along the abattoir-to-table food chain, coupled with the habit of raw meat consumption, this result indicates a potential public and animal health risk from the pathogen and antimicrobial resistance. 2024-05-06 2024-05-09T08:23:52Z 2024-05-09T08:23:52Z Journal Article https://hdl.handle.net/10568/141786 en Open Access Springer Abey, S.L., Teka, M., Bitew, A.B., Molla, W., Ejo, M., Dagnaw, G.G., Adugna, T., Nigatu, S., Mengistu, B.A., Kinde, M.Z., Berju, A., Belete, M.A., Temesgen, W., Dagnachew, S. and Tesema, T.S. 2024. Detection and antibiogram profile of diarrheagenic Escherichia coli isolated from two abattoir settings in northwest Ethiopia: a one health perspective. One Health Outlook 6: 8. |
| spellingShingle | antimicrobial resistance one health approach Abey, S.L. Teka, M. Bitew, A.B. Molla, W. Ejo, M. Dagnaw, G.G. Adugna, T. Nigatu, S. Mengistu, B.A. Kinde, M.Z. Berju, A. Belete, M.A. Temesgen, Wudu Dagnachew, S. Tesema, T.S. Detection and antibiogram profile of diarrheagenic Escherichia coli isolated from two abattoir settings in northwest Ethiopia: a one health perspective |
| title | Detection and antibiogram profile of diarrheagenic Escherichia coli isolated from two abattoir settings in northwest Ethiopia: a one health perspective |
| title_full | Detection and antibiogram profile of diarrheagenic Escherichia coli isolated from two abattoir settings in northwest Ethiopia: a one health perspective |
| title_fullStr | Detection and antibiogram profile of diarrheagenic Escherichia coli isolated from two abattoir settings in northwest Ethiopia: a one health perspective |
| title_full_unstemmed | Detection and antibiogram profile of diarrheagenic Escherichia coli isolated from two abattoir settings in northwest Ethiopia: a one health perspective |
| title_short | Detection and antibiogram profile of diarrheagenic Escherichia coli isolated from two abattoir settings in northwest Ethiopia: a one health perspective |
| title_sort | detection and antibiogram profile of diarrheagenic escherichia coli isolated from two abattoir settings in northwest ethiopia a one health perspective |
| topic | antimicrobial resistance one health approach |
| url | https://hdl.handle.net/10568/141786 |
| work_keys_str_mv | AT abeysl detectionandantibiogramprofileofdiarrheagenicescherichiacoliisolatedfromtwoabattoirsettingsinnorthwestethiopiaaonehealthperspective AT tekam detectionandantibiogramprofileofdiarrheagenicescherichiacoliisolatedfromtwoabattoirsettingsinnorthwestethiopiaaonehealthperspective AT bitewab detectionandantibiogramprofileofdiarrheagenicescherichiacoliisolatedfromtwoabattoirsettingsinnorthwestethiopiaaonehealthperspective AT mollaw detectionandantibiogramprofileofdiarrheagenicescherichiacoliisolatedfromtwoabattoirsettingsinnorthwestethiopiaaonehealthperspective AT ejom detectionandantibiogramprofileofdiarrheagenicescherichiacoliisolatedfromtwoabattoirsettingsinnorthwestethiopiaaonehealthperspective AT dagnawgg detectionandantibiogramprofileofdiarrheagenicescherichiacoliisolatedfromtwoabattoirsettingsinnorthwestethiopiaaonehealthperspective AT adugnat detectionandantibiogramprofileofdiarrheagenicescherichiacoliisolatedfromtwoabattoirsettingsinnorthwestethiopiaaonehealthperspective AT nigatus detectionandantibiogramprofileofdiarrheagenicescherichiacoliisolatedfromtwoabattoirsettingsinnorthwestethiopiaaonehealthperspective AT mengistuba detectionandantibiogramprofileofdiarrheagenicescherichiacoliisolatedfromtwoabattoirsettingsinnorthwestethiopiaaonehealthperspective AT kindemz detectionandantibiogramprofileofdiarrheagenicescherichiacoliisolatedfromtwoabattoirsettingsinnorthwestethiopiaaonehealthperspective AT berjua detectionandantibiogramprofileofdiarrheagenicescherichiacoliisolatedfromtwoabattoirsettingsinnorthwestethiopiaaonehealthperspective AT beletema detectionandantibiogramprofileofdiarrheagenicescherichiacoliisolatedfromtwoabattoirsettingsinnorthwestethiopiaaonehealthperspective AT temesgenwudu detectionandantibiogramprofileofdiarrheagenicescherichiacoliisolatedfromtwoabattoirsettingsinnorthwestethiopiaaonehealthperspective AT dagnachews detectionandantibiogramprofileofdiarrheagenicescherichiacoliisolatedfromtwoabattoirsettingsinnorthwestethiopiaaonehealthperspective AT tesemats detectionandantibiogramprofileofdiarrheagenicescherichiacoliisolatedfromtwoabattoirsettingsinnorthwestethiopiaaonehealthperspective |