Influence of genotypes and crop growth stage at time of infection on seed transmission of Maize chlorotic mottle virus

Understanding the effect of seed transmission of Maize chlorotic mottle virus (MCMV) is important in managing the spread of the disease especially secondary transmission caused by vectors. This study was set out to investigate the effect of different genotypes and crop growth stage at the time of in...

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Autores principales: Kimani, Esther, Cyrus Mugambi Micheni, Laureen Gatwiri Muriki, Samuel Mwaura Kiarie, Miano, Douglas Watuku, Macharia, Isaac, Muiru, William Maina, Prasanna, Boddupalli M., Wangai, Anne
Formato: Journal Article
Lenguaje:Inglés
Publicado: Informa UK Limited 2024
Materias:
Acceso en línea:https://hdl.handle.net/10568/141578
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author Kimani, Esther
Cyrus Mugambi Micheni
Laureen Gatwiri Muriki
Samuel Mwaura Kiarie
Miano, Douglas Watuku
Macharia, Isaac
Muiru, William Maina
Prasanna, Boddupalli M.
Wangai, Anne
author_browse Cyrus Mugambi Micheni
Kimani, Esther
Laureen Gatwiri Muriki
Macharia, Isaac
Miano, Douglas Watuku
Muiru, William Maina
Prasanna, Boddupalli M.
Samuel Mwaura Kiarie
Wangai, Anne
author_facet Kimani, Esther
Cyrus Mugambi Micheni
Laureen Gatwiri Muriki
Samuel Mwaura Kiarie
Miano, Douglas Watuku
Macharia, Isaac
Muiru, William Maina
Prasanna, Boddupalli M.
Wangai, Anne
author_sort Kimani, Esther
collection Repository of Agricultural Research Outputs (CGSpace)
description Understanding the effect of seed transmission of Maize chlorotic mottle virus (MCMV) is important in managing the spread of the disease especially secondary transmission caused by vectors. This study was set out to investigate the effect of different genotypes and crop growth stage at the time of infection to rate of MCMV transmission from seeds to seedlings. The experiments were laid out in randomised complete block design with three replications. Detection of MCMV in the grow outs was determined using double antibody sandwich enzyme-linked immunosorbent assay and real time reverse transcription polymerase chain reaction. The results showed low rates of seed transmission from the genotypes in which their was detection of MCMV in their samples (0.08-1%). Similarly, crop growth stage at time of infection also showed low transmission rate of up to 0.1%, with highest transmission rate at the four-leaf stage.
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spelling CGSpace1415782025-10-26T12:50:57Z Influence of genotypes and crop growth stage at time of infection on seed transmission of Maize chlorotic mottle virus Kimani, Esther Cyrus Mugambi Micheni Laureen Gatwiri Muriki Samuel Mwaura Kiarie Miano, Douglas Watuku Macharia, Isaac Muiru, William Maina Prasanna, Boddupalli M. Wangai, Anne maize chlorotic mottle virus maize necrosis seed genotypes crop growth rate Understanding the effect of seed transmission of Maize chlorotic mottle virus (MCMV) is important in managing the spread of the disease especially secondary transmission caused by vectors. This study was set out to investigate the effect of different genotypes and crop growth stage at the time of infection to rate of MCMV transmission from seeds to seedlings. The experiments were laid out in randomised complete block design with three replications. Detection of MCMV in the grow outs was determined using double antibody sandwich enzyme-linked immunosorbent assay and real time reverse transcription polymerase chain reaction. The results showed low rates of seed transmission from the genotypes in which their was detection of MCMV in their samples (0.08-1%). Similarly, crop growth stage at time of infection also showed low transmission rate of up to 0.1%, with highest transmission rate at the four-leaf stage. 2024-01-20 2024-04-23T15:14:47Z 2024-04-23T15:14:47Z Journal Article https://hdl.handle.net/10568/141578 en Open Access application/pdf Informa UK Limited Kimani, E. N., Micheni, C. M., Muriki, L. G., Kiarie, S. M., Miano, D. W., Macharia, I., Muiru, W. M., Prasanna, B., & Wangai, A. (2024). Influence of genotypes and crop growth stage at time of infection on seed transmission of Maize chlorotic mottle virus. Archives of Phytopathology and Plant Protection, 1–17. https://doi.org/10.1080/03235408.2024.2331040
spellingShingle maize chlorotic mottle virus
maize
necrosis
seed
genotypes
crop growth rate
Kimani, Esther
Cyrus Mugambi Micheni
Laureen Gatwiri Muriki
Samuel Mwaura Kiarie
Miano, Douglas Watuku
Macharia, Isaac
Muiru, William Maina
Prasanna, Boddupalli M.
Wangai, Anne
Influence of genotypes and crop growth stage at time of infection on seed transmission of Maize chlorotic mottle virus
title Influence of genotypes and crop growth stage at time of infection on seed transmission of Maize chlorotic mottle virus
title_full Influence of genotypes and crop growth stage at time of infection on seed transmission of Maize chlorotic mottle virus
title_fullStr Influence of genotypes and crop growth stage at time of infection on seed transmission of Maize chlorotic mottle virus
title_full_unstemmed Influence of genotypes and crop growth stage at time of infection on seed transmission of Maize chlorotic mottle virus
title_short Influence of genotypes and crop growth stage at time of infection on seed transmission of Maize chlorotic mottle virus
title_sort influence of genotypes and crop growth stage at time of infection on seed transmission of maize chlorotic mottle virus
topic maize chlorotic mottle virus
maize
necrosis
seed
genotypes
crop growth rate
url https://hdl.handle.net/10568/141578
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