Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes

East Coast fever, caused by the tick-borne intracellular apicomplexan parasite Theileria parva, is a highly fatal lymphoproliferative disease of cattle. The pathogenic schizont-induced lymphocyte transformation is a unique cancer-like condition that is reversible with parasite removal. Schizont-infe...

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Autores principales: Graham, S.P., Pelle, Roger, Honda, Y., Mwangi, David Miano, Tonukari, N.J., Yamage, M., Glew, E.J., Villiers, Etienne P. de
Formato: Journal Article
Lenguaje:Inglés
Publicado: National Academy of Sciences 2006
Materias:
Acceso en línea:https://hdl.handle.net/10568/1406
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author Graham, S.P.
Pelle, Roger
Honda, Y.
Mwangi, David Miano
Tonukari, N.J.
Yamage, M.
Glew, E.J.
Villiers, Etienne P. de
author_browse Glew, E.J.
Graham, S.P.
Honda, Y.
Mwangi, David Miano
Pelle, Roger
Tonukari, N.J.
Villiers, Etienne P. de
Yamage, M.
author_facet Graham, S.P.
Pelle, Roger
Honda, Y.
Mwangi, David Miano
Tonukari, N.J.
Yamage, M.
Glew, E.J.
Villiers, Etienne P. de
author_sort Graham, S.P.
collection Repository of Agricultural Research Outputs (CGSpace)
description East Coast fever, caused by the tick-borne intracellular apicomplexan parasite Theileria parva, is a highly fatal lymphoproliferative disease of cattle. The pathogenic schizont-induced lymphocyte transformation is a unique cancer-like condition that is reversible with parasite removal. Schizont-infected cell-directed CD8+ cytotoxic T lymphocytes (CTL) constitute the dominant protective bovine immune response after a single exposure to infection. However, the schizont antigens targeted by T. parva-specific CTL are undefined. Here we show the identification of five candidate vaccine antigens that are the targets of MHC class I-restricted CD8+ CTL from immune cattle. CD8+ T cell responses to these antigens were boosted in T. parva-immune cattle resolving a challenge infection and, when used to immunize naïve cattle, induced CTL responses that significantly correlated with survival from a lethal parasite challenge. These data provide a basis for developing a CTL-targeted anti-East Coast fever subunit vaccine. In addition, orthologs of these antigens may be vaccine targets for other apicomplexan parasites.
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spelling CGSpace14062024-10-17T09:47:53Z Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes Graham, S.P. Pelle, Roger Honda, Y. Mwangi, David Miano Tonukari, N.J. Yamage, M. Glew, E.J. Villiers, Etienne P. de animal diseases disease control East Coast fever, caused by the tick-borne intracellular apicomplexan parasite Theileria parva, is a highly fatal lymphoproliferative disease of cattle. The pathogenic schizont-induced lymphocyte transformation is a unique cancer-like condition that is reversible with parasite removal. Schizont-infected cell-directed CD8+ cytotoxic T lymphocytes (CTL) constitute the dominant protective bovine immune response after a single exposure to infection. However, the schizont antigens targeted by T. parva-specific CTL are undefined. Here we show the identification of five candidate vaccine antigens that are the targets of MHC class I-restricted CD8+ CTL from immune cattle. CD8+ T cell responses to these antigens were boosted in T. parva-immune cattle resolving a challenge infection and, when used to immunize naïve cattle, induced CTL responses that significantly correlated with survival from a lethal parasite challenge. These data provide a basis for developing a CTL-targeted anti-East Coast fever subunit vaccine. In addition, orthologs of these antigens may be vaccine targets for other apicomplexan parasites. 2006-02-28 2010-05-07T11:05:01Z 2010-05-07T11:05:01Z Journal Article https://hdl.handle.net/10568/1406 en Open Access National Academy of Sciences Graham S., et al. 2006. Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes. Proceedings of the National Academy of Sciences of the United States of America (PNAS) 103(9):3286-3291.
spellingShingle animal diseases
disease control
Graham, S.P.
Pelle, Roger
Honda, Y.
Mwangi, David Miano
Tonukari, N.J.
Yamage, M.
Glew, E.J.
Villiers, Etienne P. de
Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes
title Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes
title_full Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes
title_fullStr Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes
title_full_unstemmed Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes
title_short Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes
title_sort theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic t lymphocytes
topic animal diseases
disease control
url https://hdl.handle.net/10568/1406
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