An alfalfa MYB‐like transcriptional factor MsMYBH positively regulates alfalfa seedling drought resistance and undergoes MsWAV3‐mediated degradation
Drought is a major threat to alfalfa (Medicago sativa L.) production. The discovery of important alfalfa genes regulating drought response will facilitate breeding for drought-resistant alfalfa cultivars. Here, we report a genome-wide association study of drought resistance in alfalfa. We identified...
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2024
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/139543 |
| _version_ | 1855543183919808512 |
|---|---|
| author | Kun Shi Jia Liu Huan Liang Hongbin Dong Jinli Zhang Yuanhong Wei Le Zhou Shaopeng Wang Jiahao Zhu Mingshu Cao Jones, Christopher S. Dongmei Ma Zan Wang |
| author_browse | Dongmei Ma Hongbin Dong Huan Liang Jia Liu Jiahao Zhu Jinli Zhang Jones, Christopher S. Kun Shi Le Zhou Mingshu Cao Shaopeng Wang Yuanhong Wei Zan Wang |
| author_facet | Kun Shi Jia Liu Huan Liang Hongbin Dong Jinli Zhang Yuanhong Wei Le Zhou Shaopeng Wang Jiahao Zhu Mingshu Cao Jones, Christopher S. Dongmei Ma Zan Wang |
| author_sort | Kun Shi |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Drought is a major threat to alfalfa (Medicago sativa L.) production. The discovery of important alfalfa genes regulating drought response will facilitate breeding for drought-resistant alfalfa cultivars. Here, we report a genome-wide association study of drought resistance in alfalfa. We identified and functionally characterized an MYB-like transcription factor gene (MsMYBH), which increases the drought resistance in alfalfa. Compared with the wild-types, the biomass and forage quality were enhanced in MsMYBH overexpressed plants. Combined RNA-seq, proteomics and chromatin immunoprecipitation analysis showed that MsMYBH can directly bind to the promoters of MsMCP1, MsMCP2, MsPRX1A and MsCARCAB to improve their expression. The outcomes of such interactions include better water balance, high photosynthetic efficiency and scavenge excess H2O2 in response to drought. Furthermore, an E3 ubiquitin ligase (MsWAV3) was found to induce MsMYBH degradation under long-term drought, via the 26S proteasome pathway. Furthermore, variable-number tandem repeats in MsMYBH promoter were characterized among a collection of germplasms, and the variation is associated with promoter activity. Collectively, our findings shed light on the functions of MsMYBH and provide a pivotal gene that could be leveraged for breeding drought-resistant alfalfa. This discovery also offers new insights into the mechanisms of drought resistance in alfalfa. |
| format | Journal Article |
| id | CGSpace139543 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1395432025-12-08T09:54:28Z An alfalfa MYB‐like transcriptional factor MsMYBH positively regulates alfalfa seedling drought resistance and undergoes MsWAV3‐mediated degradation Kun Shi Jia Liu Huan Liang Hongbin Dong Jinli Zhang Yuanhong Wei Le Zhou Shaopeng Wang Jiahao Zhu Mingshu Cao Jones, Christopher S. Dongmei Ma Zan Wang Medicago sativa drought resistance seedlings plant breeding Drought is a major threat to alfalfa (Medicago sativa L.) production. The discovery of important alfalfa genes regulating drought response will facilitate breeding for drought-resistant alfalfa cultivars. Here, we report a genome-wide association study of drought resistance in alfalfa. We identified and functionally characterized an MYB-like transcription factor gene (MsMYBH), which increases the drought resistance in alfalfa. Compared with the wild-types, the biomass and forage quality were enhanced in MsMYBH overexpressed plants. Combined RNA-seq, proteomics and chromatin immunoprecipitation analysis showed that MsMYBH can directly bind to the promoters of MsMCP1, MsMCP2, MsPRX1A and MsCARCAB to improve their expression. The outcomes of such interactions include better water balance, high photosynthetic efficiency and scavenge excess H2O2 in response to drought. Furthermore, an E3 ubiquitin ligase (MsWAV3) was found to induce MsMYBH degradation under long-term drought, via the 26S proteasome pathway. Furthermore, variable-number tandem repeats in MsMYBH promoter were characterized among a collection of germplasms, and the variation is associated with promoter activity. Collectively, our findings shed light on the functions of MsMYBH and provide a pivotal gene that could be leveraged for breeding drought-resistant alfalfa. This discovery also offers new insights into the mechanisms of drought resistance in alfalfa. 2024-04 2024-02-20T17:48:06Z 2024-02-20T17:48:06Z Journal Article https://hdl.handle.net/10568/139543 en Open Access Wiley Shi, K., Liu, J., Liang, H., Dong, H., Zhang, J., Wei, Y., Zhou, L., Wang, S., Zhu, J., Cao, M., Jones, C.S., Ma, D. and Wang, Z. 2024. An alfalfa MYB-like transcriptional factor MsMYBH positively regulates alfalfa seedling drought resistance and undergoes MsWAV3-mediated degradation. Journal of Integrative Plant Biology 66(4): 683-699. |
| spellingShingle | Medicago sativa drought resistance seedlings plant breeding Kun Shi Jia Liu Huan Liang Hongbin Dong Jinli Zhang Yuanhong Wei Le Zhou Shaopeng Wang Jiahao Zhu Mingshu Cao Jones, Christopher S. Dongmei Ma Zan Wang An alfalfa MYB‐like transcriptional factor MsMYBH positively regulates alfalfa seedling drought resistance and undergoes MsWAV3‐mediated degradation |
| title | An alfalfa MYB‐like transcriptional factor MsMYBH positively regulates alfalfa seedling drought resistance and undergoes MsWAV3‐mediated degradation |
| title_full | An alfalfa MYB‐like transcriptional factor MsMYBH positively regulates alfalfa seedling drought resistance and undergoes MsWAV3‐mediated degradation |
| title_fullStr | An alfalfa MYB‐like transcriptional factor MsMYBH positively regulates alfalfa seedling drought resistance and undergoes MsWAV3‐mediated degradation |
| title_full_unstemmed | An alfalfa MYB‐like transcriptional factor MsMYBH positively regulates alfalfa seedling drought resistance and undergoes MsWAV3‐mediated degradation |
| title_short | An alfalfa MYB‐like transcriptional factor MsMYBH positively regulates alfalfa seedling drought resistance and undergoes MsWAV3‐mediated degradation |
| title_sort | alfalfa myb like transcriptional factor msmybh positively regulates alfalfa seedling drought resistance and undergoes mswav3 mediated degradation |
| topic | Medicago sativa drought resistance seedlings plant breeding |
| url | https://hdl.handle.net/10568/139543 |
| work_keys_str_mv | AT kunshi analfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT jialiu analfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT huanliang analfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT hongbindong analfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT jinlizhang analfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT yuanhongwei analfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT lezhou analfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT shaopengwang analfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT jiahaozhu analfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT mingshucao analfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT joneschristophers analfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT dongmeima analfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT zanwang analfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT kunshi alfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT jialiu alfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT huanliang alfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT hongbindong alfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT jinlizhang alfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT yuanhongwei alfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT lezhou alfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT shaopengwang alfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT jiahaozhu alfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT mingshucao alfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT joneschristophers alfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT dongmeima alfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation AT zanwang alfalfamybliketranscriptionalfactormsmybhpositivelyregulatesalfalfaseedlingdroughtresistanceandundergoesmswav3mediateddegradation |