Evaluation of in situ and ex situ forage germplasm collections reveals the first occurrence and seed-transmission of alfalfa mosaic virus and southern bean mosaic virus in Urochloa species
Grasses of the genus Urochloa (Urochloa species syn. Brachiaria species) are among the most important tropical forage grasses of African origin. The International Livestock Research Institute (ILRI) Forage Genebank maintains 671 Urochloa grass accessions, belonging to 28 species, of which 261 (39%)...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Taylor and Francis
2024
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/138520 |
| _version_ | 1855516985646907392 |
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| author | Dawit, Woubit Mulatu, Fikerte Eshete, Yesuf Negawo, Alemayehu T. Kumar, Alok Muchugi, Alice Jones, Christopher S. |
| author_browse | Dawit, Woubit Eshete, Yesuf Jones, Christopher S. Kumar, Alok Muchugi, Alice Mulatu, Fikerte Negawo, Alemayehu T. |
| author_facet | Dawit, Woubit Mulatu, Fikerte Eshete, Yesuf Negawo, Alemayehu T. Kumar, Alok Muchugi, Alice Jones, Christopher S. |
| author_sort | Dawit, Woubit |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Grasses of the genus Urochloa (Urochloa species syn. Brachiaria species) are among the most important tropical forage grasses of African origin. The International Livestock Research Institute (ILRI) Forage Genebank maintains 671 Urochloa grass accessions, belonging to 28 species, of which 261 (39%) accessions are Urochloa brizantha (A.Rich.) R.D.Webster. The susceptibility of Urochloa grasses to disease and insect pests is a key challenge to the sustainable production of these grasses in Africa. In this study, using a dot blot assay and RT-PCR, we report for the first time the association of Alfalfa Mosaic Virus (AMV) and Southern Bean Mosaic Virus (SBMV) with 92% of the tested accessions conserved in situ and ex situ in the ILRI forage genebank. In addition, virus transmission from seed to plant and from legume to grass was confirmed through the bioassay test. The detection of these two viruses in Urochloa species presents a new challenge to germplasm conservation, distribution, and their sustainable production in the region, and demands immediate attention towards developing an effective method of cleaning viruses from the conserved germplasm. Furthermore, there is an urgent need to assess the economic importance of diseases in the grasses, and for the selection of germplasm that shows resistance to diseases. |
| format | Journal Article |
| id | CGSpace138520 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Taylor and Francis |
| publisherStr | Taylor and Francis |
| record_format | dspace |
| spelling | CGSpace1385202025-11-13T10:38:22Z Evaluation of in situ and ex situ forage germplasm collections reveals the first occurrence and seed-transmission of alfalfa mosaic virus and southern bean mosaic virus in Urochloa species Dawit, Woubit Mulatu, Fikerte Eshete, Yesuf Negawo, Alemayehu T. Kumar, Alok Muchugi, Alice Jones, Christopher S. amv forage diseases sbmv urochloa grasses Grasses of the genus Urochloa (Urochloa species syn. Brachiaria species) are among the most important tropical forage grasses of African origin. The International Livestock Research Institute (ILRI) Forage Genebank maintains 671 Urochloa grass accessions, belonging to 28 species, of which 261 (39%) accessions are Urochloa brizantha (A.Rich.) R.D.Webster. The susceptibility of Urochloa grasses to disease and insect pests is a key challenge to the sustainable production of these grasses in Africa. In this study, using a dot blot assay and RT-PCR, we report for the first time the association of Alfalfa Mosaic Virus (AMV) and Southern Bean Mosaic Virus (SBMV) with 92% of the tested accessions conserved in situ and ex situ in the ILRI forage genebank. In addition, virus transmission from seed to plant and from legume to grass was confirmed through the bioassay test. The detection of these two viruses in Urochloa species presents a new challenge to germplasm conservation, distribution, and their sustainable production in the region, and demands immediate attention towards developing an effective method of cleaning viruses from the conserved germplasm. Furthermore, there is an urgent need to assess the economic importance of diseases in the grasses, and for the selection of germplasm that shows resistance to diseases. 2024-06-03 2024-01-25T17:37:49Z 2024-01-25T17:37:49Z Journal Article https://hdl.handle.net/10568/138520 en Open Access Taylor and Francis Dawit, W., Mulatu, F., Eshete, Y, Negawo, A.T., Kumar, A., Muchugi, A & Jones, C.S. 2024. Evaluation of in situ and ex situ forage germplasm collections reveals the first occurrence and seed-transmission of alfalfa mosaic virus and southern bean mosaic virus in Urochloa species, African Journal of Range and Forage Science |
| spellingShingle | amv forage diseases sbmv urochloa grasses Dawit, Woubit Mulatu, Fikerte Eshete, Yesuf Negawo, Alemayehu T. Kumar, Alok Muchugi, Alice Jones, Christopher S. Evaluation of in situ and ex situ forage germplasm collections reveals the first occurrence and seed-transmission of alfalfa mosaic virus and southern bean mosaic virus in Urochloa species |
| title | Evaluation of in situ and ex situ forage germplasm collections reveals the first occurrence and seed-transmission of alfalfa mosaic virus and southern bean mosaic virus in Urochloa species |
| title_full | Evaluation of in situ and ex situ forage germplasm collections reveals the first occurrence and seed-transmission of alfalfa mosaic virus and southern bean mosaic virus in Urochloa species |
| title_fullStr | Evaluation of in situ and ex situ forage germplasm collections reveals the first occurrence and seed-transmission of alfalfa mosaic virus and southern bean mosaic virus in Urochloa species |
| title_full_unstemmed | Evaluation of in situ and ex situ forage germplasm collections reveals the first occurrence and seed-transmission of alfalfa mosaic virus and southern bean mosaic virus in Urochloa species |
| title_short | Evaluation of in situ and ex situ forage germplasm collections reveals the first occurrence and seed-transmission of alfalfa mosaic virus and southern bean mosaic virus in Urochloa species |
| title_sort | evaluation of in situ and ex situ forage germplasm collections reveals the first occurrence and seed transmission of alfalfa mosaic virus and southern bean mosaic virus in urochloa species |
| topic | amv forage diseases sbmv urochloa grasses |
| url | https://hdl.handle.net/10568/138520 |
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