Genome-wide association analysis of tan spot disease resistance in durum wheat accessions from Tunisia
Background: Tunisia harbors a rich collection of unexploited durum wheat landraces (Triticum durum ssp. durum) that have been gradually replaced by elite cultivars since the 1970s. These landraces represent an important potential source for broadening the genetic background of elite durum wheat cult...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/137922 |
| _version_ | 1855523678895210496 |
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| author | Laribi, Marwa Fredua-Agyeman, Rudolph Ben M’Barek, Sarrah Sansaloni, Carolina P. Dreisigacker, Susanne Gamba, Fernanda M. Abdedayem, Wided Nefzaoui, Meriem Araar, Chayma Sheau-Fang Hwang Yahyaoui, Amor H. Strelkov, Stephen E. |
| author_browse | Abdedayem, Wided Araar, Chayma Ben M’Barek, Sarrah Dreisigacker, Susanne Fredua-Agyeman, Rudolph Gamba, Fernanda M. Laribi, Marwa Nefzaoui, Meriem Sansaloni, Carolina P. Sheau-Fang Hwang Strelkov, Stephen E. Yahyaoui, Amor H. |
| author_facet | Laribi, Marwa Fredua-Agyeman, Rudolph Ben M’Barek, Sarrah Sansaloni, Carolina P. Dreisigacker, Susanne Gamba, Fernanda M. Abdedayem, Wided Nefzaoui, Meriem Araar, Chayma Sheau-Fang Hwang Yahyaoui, Amor H. Strelkov, Stephen E. |
| author_sort | Laribi, Marwa |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Background: Tunisia harbors a rich collection of unexploited durum wheat landraces (Triticum durum ssp. durum) that have been gradually replaced by elite cultivars since the 1970s. These landraces represent an important potential source for broadening the genetic background of elite durum wheat cultivars and for the introgression of novel genes for key traits, including disease resistance, into these cultivars. Methods: In this study, single nucleotide polymorphism (SNP) markers were used to investigate the genetic diversity and population structure of a core collection of 235 durum wheat accessions consisting mainly of landraces. The high phenotypic and genetic diversity of the fungal pathogen Pyrenophora tritici-repentis (cause of tan spot disease of wheat) in Tunisia allowed the assessment of the accessions for tan spot resistance at the adult plant stage under field conditions over three cropping seasons. A genome-wide association study (GWAS) was performed using a 90k SNP array. Results: Bayesian population structure analysis with 9191 polymorphic SNP markers classified the accessions into two groups, where groups 1 and 2 included 49.79% and 31.49% of the accessions, respectively, while the remaining 18.72% were admixtures. Principal coordinate analysis, the unweighted pair group method with arithmetic mean and the neighbor-joining method clustered the accessions into three to five groups. Analysis of molecular variance indicated that 76% of the genetic variation was among individuals and 23% was between individuals. Genome-wide association analyses identified 26 SNPs associated with tan spot resistance and explained between 8.1% to 20.2% of the phenotypic variation. The SNPs were located on chromosomes 1B (1 SNP), 2B (4 SNPs), 3A (2 SNPs), 3B (2 SNPs), 4A (2 SNPs), 4B (1 SNP), 5A (2 SNPs), 5B (4 SNPs), 6A (5 SNPs), 6B (2 SNPs), and 7B (1 SNP). Four markers, one on each of chromosomes 1B, and 5A, and two on 5B, coincided with previously reported SNPs for tan spot resistance, while the remaining SNPs were either novel markers or closely related to previously reported SNPs. Eight durum wheat accessions were identified as possible novel sources of tan spot resistance that could be introgressed into elite cultivars. Conclusion: The results highlighted the significance of chromosomes 2B, 5B, and 6A as genomic regions associated with tan spot resistance. |
| format | Journal Article |
| id | CGSpace137922 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1379222025-12-08T10:29:22Z Genome-wide association analysis of tan spot disease resistance in durum wheat accessions from Tunisia Laribi, Marwa Fredua-Agyeman, Rudolph Ben M’Barek, Sarrah Sansaloni, Carolina P. Dreisigacker, Susanne Gamba, Fernanda M. Abdedayem, Wided Nefzaoui, Meriem Araar, Chayma Sheau-Fang Hwang Yahyaoui, Amor H. Strelkov, Stephen E. disease resistance hard wheat genetic diversity genome-wide association studies landraces population structure Background: Tunisia harbors a rich collection of unexploited durum wheat landraces (Triticum durum ssp. durum) that have been gradually replaced by elite cultivars since the 1970s. These landraces represent an important potential source for broadening the genetic background of elite durum wheat cultivars and for the introgression of novel genes for key traits, including disease resistance, into these cultivars. Methods: In this study, single nucleotide polymorphism (SNP) markers were used to investigate the genetic diversity and population structure of a core collection of 235 durum wheat accessions consisting mainly of landraces. The high phenotypic and genetic diversity of the fungal pathogen Pyrenophora tritici-repentis (cause of tan spot disease of wheat) in Tunisia allowed the assessment of the accessions for tan spot resistance at the adult plant stage under field conditions over three cropping seasons. A genome-wide association study (GWAS) was performed using a 90k SNP array. Results: Bayesian population structure analysis with 9191 polymorphic SNP markers classified the accessions into two groups, where groups 1 and 2 included 49.79% and 31.49% of the accessions, respectively, while the remaining 18.72% were admixtures. Principal coordinate analysis, the unweighted pair group method with arithmetic mean and the neighbor-joining method clustered the accessions into three to five groups. Analysis of molecular variance indicated that 76% of the genetic variation was among individuals and 23% was between individuals. Genome-wide association analyses identified 26 SNPs associated with tan spot resistance and explained between 8.1% to 20.2% of the phenotypic variation. The SNPs were located on chromosomes 1B (1 SNP), 2B (4 SNPs), 3A (2 SNPs), 3B (2 SNPs), 4A (2 SNPs), 4B (1 SNP), 5A (2 SNPs), 5B (4 SNPs), 6A (5 SNPs), 6B (2 SNPs), and 7B (1 SNP). Four markers, one on each of chromosomes 1B, and 5A, and two on 5B, coincided with previously reported SNPs for tan spot resistance, while the remaining SNPs were either novel markers or closely related to previously reported SNPs. Eight durum wheat accessions were identified as possible novel sources of tan spot resistance that could be introgressed into elite cultivars. Conclusion: The results highlighted the significance of chromosomes 2B, 5B, and 6A as genomic regions associated with tan spot resistance. 2023 2024-01-17T21:29:15Z 2024-01-17T21:29:15Z Journal Article https://hdl.handle.net/10568/137922 en Open Access application/pdf Frontiers Media Laribi, M., Fredua-Agyeman, R., Ben M’Barek, S., Sansaloni, C. P., Dreisigacker, S., Gamba, F. M., Abdedayem, W., Nefzaoui, M., Araar, C., Hwang, S.-F., Yahyaoui, A. H., & Strelkov, S. E. (2023). Genome-wide association analysis of tan spot disease resistance in durum wheat accessions from Tunisia. Frontiers in Genetics, 14. https://doi.org/10.3389/fgene.2023.1231027 |
| spellingShingle | disease resistance hard wheat genetic diversity genome-wide association studies landraces population structure Laribi, Marwa Fredua-Agyeman, Rudolph Ben M’Barek, Sarrah Sansaloni, Carolina P. Dreisigacker, Susanne Gamba, Fernanda M. Abdedayem, Wided Nefzaoui, Meriem Araar, Chayma Sheau-Fang Hwang Yahyaoui, Amor H. Strelkov, Stephen E. Genome-wide association analysis of tan spot disease resistance in durum wheat accessions from Tunisia |
| title | Genome-wide association analysis of tan spot disease resistance in durum wheat accessions from Tunisia |
| title_full | Genome-wide association analysis of tan spot disease resistance in durum wheat accessions from Tunisia |
| title_fullStr | Genome-wide association analysis of tan spot disease resistance in durum wheat accessions from Tunisia |
| title_full_unstemmed | Genome-wide association analysis of tan spot disease resistance in durum wheat accessions from Tunisia |
| title_short | Genome-wide association analysis of tan spot disease resistance in durum wheat accessions from Tunisia |
| title_sort | genome wide association analysis of tan spot disease resistance in durum wheat accessions from tunisia |
| topic | disease resistance hard wheat genetic diversity genome-wide association studies landraces population structure |
| url | https://hdl.handle.net/10568/137922 |
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