Maize ZmBES1/BZR1-1 transcription factor negatively regulates drought tolerance
Drought stress is a common abiotic factor and restricts plant growth and development. Exploring maize stress-related genes and their regulatory mechanisms is crucial for ensuring agricultural productivity and food security. The BRI1-EMS1 suppressor (BES1)/brassinazole-resistant 1 (BZR1) transcriptio...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/137828 |
| _version_ | 1855531776648151040 |
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| author | Wenqi Feng Hongwanjun Zhang Yang Cao Yuan Liu Yiran Zhao Fuai Sun Qingqing Yang Xuecai Zhang Yuanyuan Zhang Yingge Wang Wanchen Li Yanli Lu Fengling Fu Haoqiang Yu |
| author_browse | Fengling Fu Fuai Sun Haoqiang Yu Hongwanjun Zhang Qingqing Yang Wanchen Li Wenqi Feng Xuecai Zhang Yang Cao Yanli Lu Yingge Wang Yiran Zhao Yuan Liu Yuanyuan Zhang |
| author_facet | Wenqi Feng Hongwanjun Zhang Yang Cao Yuan Liu Yiran Zhao Fuai Sun Qingqing Yang Xuecai Zhang Yuanyuan Zhang Yingge Wang Wanchen Li Yanli Lu Fengling Fu Haoqiang Yu |
| author_sort | Wenqi Feng |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Drought stress is a common abiotic factor and restricts plant growth and development. Exploring maize stress-related genes and their regulatory mechanisms is crucial for ensuring agricultural productivity and food security. The BRI1-EMS1 suppressor (BES1)/brassinazole-resistant 1 (BZR1) transcription factors (TFs) play important roles in plant growth, development, and stress response. However, maize ZmBES1/BZR1s are rarely reported. In the present study, the ZmBES1/BZR1-1 gene was cloned from maize B73 and functionally characterized in transgenic Arabidopsis and rice in drought stress response. The ZmBES1/BZR1-1 protein possessed a conserved bHLH domain characterized by BES1/BZR1 TFs, localized in the nucleus, and showed transcription activation activity. The expression of ZmBES1/BZR1-1 exhibited no tissue specificity but drought-inhibitory expression in maize. Under drought stress, overexpression of ZmBES1/BZR1-1 resulted in the enhancement of drought sensitivity of transgenic Arabidopsis and rice with a lower survival rate, reactive oxygen species (ROS) level and relative water content (RWC) and a higher stomatal aperture and relative electrolyte leakage (REL). The RNA-seq results showed that 56 differentially expressed genes (DEGs) were regulated by ZmBES1/BZR1-1 by binding to E-box elements in their promoters. The GO analysis showed that the DEGs were significantly annotated with response to oxidative stress and oxygen level. The study suggests that the ZmBES1/BZR1-1 gene negatively regulates drought stress, which provides insights into further underlying molecular mechanisms in the drought stress response mediated by BZR1/BES1s. |
| format | Journal Article |
| id | CGSpace137828 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1378282025-10-26T12:56:11Z Maize ZmBES1/BZR1-1 transcription factor negatively regulates drought tolerance Wenqi Feng Hongwanjun Zhang Yang Cao Yuan Liu Yiran Zhao Fuai Sun Qingqing Yang Xuecai Zhang Yuanyuan Zhang Yingge Wang Wanchen Li Yanli Lu Fengling Fu Haoqiang Yu abiotic stress drought stress maize transcription factors Drought stress is a common abiotic factor and restricts plant growth and development. Exploring maize stress-related genes and their regulatory mechanisms is crucial for ensuring agricultural productivity and food security. The BRI1-EMS1 suppressor (BES1)/brassinazole-resistant 1 (BZR1) transcription factors (TFs) play important roles in plant growth, development, and stress response. However, maize ZmBES1/BZR1s are rarely reported. In the present study, the ZmBES1/BZR1-1 gene was cloned from maize B73 and functionally characterized in transgenic Arabidopsis and rice in drought stress response. The ZmBES1/BZR1-1 protein possessed a conserved bHLH domain characterized by BES1/BZR1 TFs, localized in the nucleus, and showed transcription activation activity. The expression of ZmBES1/BZR1-1 exhibited no tissue specificity but drought-inhibitory expression in maize. Under drought stress, overexpression of ZmBES1/BZR1-1 resulted in the enhancement of drought sensitivity of transgenic Arabidopsis and rice with a lower survival rate, reactive oxygen species (ROS) level and relative water content (RWC) and a higher stomatal aperture and relative electrolyte leakage (REL). The RNA-seq results showed that 56 differentially expressed genes (DEGs) were regulated by ZmBES1/BZR1-1 by binding to E-box elements in their promoters. The GO analysis showed that the DEGs were significantly annotated with response to oxidative stress and oxygen level. The study suggests that the ZmBES1/BZR1-1 gene negatively regulates drought stress, which provides insights into further underlying molecular mechanisms in the drought stress response mediated by BZR1/BES1s. 2023-12 2024-01-16T23:09:17Z 2024-01-16T23:09:17Z Journal Article https://hdl.handle.net/10568/137828 en Limited Access Elsevier Feng, W., Zhang, H., Cao, Y., Liu, Y., Zhao, Y., Sun, F., Yang, Q., Zhang, X., Zhang, Y., Wang, Y., Li, W., Lu, Y., Fu, F., & Yu, H. (2023). Maize ZmBES1/BZR1-1 transcription factor negatively regulates drought tolerance. Plant Physiology and Biochemistry, 205, 108188. |
| spellingShingle | abiotic stress drought stress maize transcription factors Wenqi Feng Hongwanjun Zhang Yang Cao Yuan Liu Yiran Zhao Fuai Sun Qingqing Yang Xuecai Zhang Yuanyuan Zhang Yingge Wang Wanchen Li Yanli Lu Fengling Fu Haoqiang Yu Maize ZmBES1/BZR1-1 transcription factor negatively regulates drought tolerance |
| title | Maize ZmBES1/BZR1-1 transcription factor negatively regulates drought tolerance |
| title_full | Maize ZmBES1/BZR1-1 transcription factor negatively regulates drought tolerance |
| title_fullStr | Maize ZmBES1/BZR1-1 transcription factor negatively regulates drought tolerance |
| title_full_unstemmed | Maize ZmBES1/BZR1-1 transcription factor negatively regulates drought tolerance |
| title_short | Maize ZmBES1/BZR1-1 transcription factor negatively regulates drought tolerance |
| title_sort | maize zmbes1 bzr1 1 transcription factor negatively regulates drought tolerance |
| topic | abiotic stress drought stress maize transcription factors |
| url | https://hdl.handle.net/10568/137828 |
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