Genetic bases of resistance to the rice hoja blanca disease deciphered by a quantitative trait locus approach
Abstract Rice hoja blanca (RHB) is one of the most serious diseases in rice-growing areas in tropical Americas. Its causal agent is RHB virus (RHBV), transmitted by the planthopper Tagosodes orizicolus Müir. Genetic resistance is the most effective and environment-friendly way of controlling the dis...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Oxford University Press
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/137789 |
| _version_ | 1855535598471741440 |
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| author | Silva, Alexander Montoya Pizarro, Maria Elker Quintero, Constanza Cuasquer, Juan Tohme, Joseph M. Graterol Matute, Eduardo Jose Cruz, Maribel Lorieux, Mathias |
| author_browse | Cruz, Maribel Cuasquer, Juan Graterol Matute, Eduardo Jose Lorieux, Mathias Montoya Pizarro, Maria Elker Quintero, Constanza Silva, Alexander Tohme, Joseph M. |
| author_facet | Silva, Alexander Montoya Pizarro, Maria Elker Quintero, Constanza Cuasquer, Juan Tohme, Joseph M. Graterol Matute, Eduardo Jose Cruz, Maribel Lorieux, Mathias |
| author_sort | Silva, Alexander |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Abstract Rice hoja blanca (RHB) is one of the most serious diseases in rice-growing areas in tropical Americas. Its causal agent is RHB virus (RHBV), transmitted by the planthopper Tagosodes orizicolus Müir. Genetic resistance is the most effective and environment-friendly way of controlling the disease. So far, only 1 major quantitative trait locus (QTL) of Oryza sativa ssp. japonica origin, qHBV4.1, that alters the incidence of the virus symptoms in 2 Colombian cultivars has been reported. This resistance has already started to be broken, stressing the urgent need for diversifying the resistance sources. In the present study, we performed a search for new QTLs of O. sativa indica origin associated with RHB resistance. We used 4 F2:3-segregating populations derived from indica-resistant varieties crossed with a highly susceptible japonica pivot parent. Besides the standard method for measuring disease incidence, we developed a new method based on computer-assisted image processing to determine the affected leaf area (ALA) as a measure of symptom severity. Based on the disease severity and incidence scores in the F3 families under greenhouse conditions and SNP genotyping of the F2 individuals, we identified 4 new indica QTLs for RHB resistance on rice chromosomes 4, 6, and 11, namely, qHBV4.2WAS208, qHBV6.1PTB25, qHBV11.1, and qHBV11.2, respectively. We also confirmed the wide-range action of qHBV4.1. Among the 5 QTLs, qHBV4.1 and qHBV11.1 had the largest effects on incidence and severity, respectively. These results provide a more complete understanding of the genetic bases of RHBV resistance in the cultivated rice gene pool and can be used to develop marker-aided breeding strategies to improve RHB resistance. The power of joint- and meta-analyses allowed precise mapping and candidate gene identification, providing the basis for positional cloning of the 2 major QTLs qHBV4.1 and qHBV11.1. |
| format | Journal Article |
| id | CGSpace137789 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace1377892025-11-11T19:02:28Z Genetic bases of resistance to the rice hoja blanca disease deciphered by a quantitative trait locus approach Silva, Alexander Montoya Pizarro, Maria Elker Quintero, Constanza Cuasquer, Juan Tohme, Joseph M. Graterol Matute, Eduardo Jose Cruz, Maribel Lorieux, Mathias genomics rice plant breeding oryza sativa quantitative trait loci rice hoja blanca virus candidate genes plant genetics Abstract Rice hoja blanca (RHB) is one of the most serious diseases in rice-growing areas in tropical Americas. Its causal agent is RHB virus (RHBV), transmitted by the planthopper Tagosodes orizicolus Müir. Genetic resistance is the most effective and environment-friendly way of controlling the disease. So far, only 1 major quantitative trait locus (QTL) of Oryza sativa ssp. japonica origin, qHBV4.1, that alters the incidence of the virus symptoms in 2 Colombian cultivars has been reported. This resistance has already started to be broken, stressing the urgent need for diversifying the resistance sources. In the present study, we performed a search for new QTLs of O. sativa indica origin associated with RHB resistance. We used 4 F2:3-segregating populations derived from indica-resistant varieties crossed with a highly susceptible japonica pivot parent. Besides the standard method for measuring disease incidence, we developed a new method based on computer-assisted image processing to determine the affected leaf area (ALA) as a measure of symptom severity. Based on the disease severity and incidence scores in the F3 families under greenhouse conditions and SNP genotyping of the F2 individuals, we identified 4 new indica QTLs for RHB resistance on rice chromosomes 4, 6, and 11, namely, qHBV4.2WAS208, qHBV6.1PTB25, qHBV11.1, and qHBV11.2, respectively. We also confirmed the wide-range action of qHBV4.1. Among the 5 QTLs, qHBV4.1 and qHBV11.1 had the largest effects on incidence and severity, respectively. These results provide a more complete understanding of the genetic bases of RHBV resistance in the cultivated rice gene pool and can be used to develop marker-aided breeding strategies to improve RHB resistance. The power of joint- and meta-analyses allowed precise mapping and candidate gene identification, providing the basis for positional cloning of the 2 major QTLs qHBV4.1 and qHBV11.1. 2023-12-06 2024-01-16T15:45:32Z 2024-01-16T15:45:32Z Journal Article https://hdl.handle.net/10568/137789 en Open Access application/pdf Oxford University Press Silva, A.; Montoya, M.E.; Quintero, C.; Cuasquer, J.; Tohme, J.; Graterol, E.; Cruz, M.; Lorieux, M. (2023) Genetic bases of resistance to the rice hoja blanca disease deciphered by a quantitative trait locus approach. G3: Genes, Genomes, Genetics 13(12): jkad223. ISSN: 2160-1836 |
| spellingShingle | genomics rice plant breeding oryza sativa quantitative trait loci rice hoja blanca virus candidate genes plant genetics Silva, Alexander Montoya Pizarro, Maria Elker Quintero, Constanza Cuasquer, Juan Tohme, Joseph M. Graterol Matute, Eduardo Jose Cruz, Maribel Lorieux, Mathias Genetic bases of resistance to the rice hoja blanca disease deciphered by a quantitative trait locus approach |
| title | Genetic bases of resistance to the rice hoja blanca disease deciphered by a quantitative trait locus approach |
| title_full | Genetic bases of resistance to the rice hoja blanca disease deciphered by a quantitative trait locus approach |
| title_fullStr | Genetic bases of resistance to the rice hoja blanca disease deciphered by a quantitative trait locus approach |
| title_full_unstemmed | Genetic bases of resistance to the rice hoja blanca disease deciphered by a quantitative trait locus approach |
| title_short | Genetic bases of resistance to the rice hoja blanca disease deciphered by a quantitative trait locus approach |
| title_sort | genetic bases of resistance to the rice hoja blanca disease deciphered by a quantitative trait locus approach |
| topic | genomics rice plant breeding oryza sativa quantitative trait loci rice hoja blanca virus candidate genes plant genetics |
| url | https://hdl.handle.net/10568/137789 |
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