Genomic regions involved in the control of 1,000-kernel weight in wild relative-derived populations of durum wheat
Terminal drought is one of the most common and devastating climatic stress factors affecting durum wheat (Triticum durum Desf.) production worldwide. The wild relatives of this crop are deemed a vast potential source of useful alleles to adapt to this stress. A nested association mapping (NAM) panel...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Frontiers Media
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/137787 |
| _version_ | 1855536019709886464 |
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| author | Jabbour, Yaman Shafik Hakim, Mohammad Al-Yossef, Abdallah M. Saleh, Maysoun Al-Dein Shaaban, Ahmad Kabbaj, Hafssa Zaim, Meryem Kleinermann, Charles Bassi, Filippo |
| author_browse | Al-Dein Shaaban, Ahmad Al-Yossef, Abdallah Bassi, Filippo Jabbour, Yaman Kabbaj, Hafssa Kleinermann, Charles M. Saleh, Maysoun Shafik Hakim, Mohammad Zaim, Meryem |
| author_facet | Jabbour, Yaman Shafik Hakim, Mohammad Al-Yossef, Abdallah M. Saleh, Maysoun Al-Dein Shaaban, Ahmad Kabbaj, Hafssa Zaim, Meryem Kleinermann, Charles Bassi, Filippo |
| author_sort | Jabbour, Yaman |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Terminal drought is one of the most common and devastating climatic stress factors affecting durum wheat (Triticum durum Desf.) production worldwide. The wild relatives of this crop are deemed a vast potential source of useful alleles to adapt to this stress. A nested association mapping (NAM) panel was generated using as a recurrent parent the Moroccan variety ‘Nachit’ derived from Triticum dicoccoides and known for its large grain size. This was recombined to three top-performing lines derived from T. dicoccoides, T. araraticum, and Aegilops speltoides, for a total of 426 inbred progenies. This NAM was evaluated across eight environments (Syria, Lebanon, and Morocco) experiencing different degrees of terminal moisture stress over two crop seasons. Our results showed that drought stress caused on average 41% loss in yield and that 1,000-kernel weight (TKW) was the most important trait for adaptation to it. Genotyping with the 25K TraitGenetics array resulted in a consensus map of 1,678 polymorphic SNPs, spanning 1,723 cM aligned to the reference ‘Svevo’ genome assembly. Kinship distinguished the progenies in three clades matching the parent of origin. A total of 18 stable quantitative trait loci (QTLs) were identified as controlling various traits but independent from flowering time. The most significant genomic regions were named Q.ICD.NAM-04, Q.ICD.NAM-14, and Q.ICD.NAM-16. Allelic investigation in a second germplasm panel confirmed that carrying the positive allele at all three loci produced an average TKW advantage of 25.6% when field-tested under drought conditions. The underlying SNPs were converted to Kompetitive Allele-Specific PCR (KASP) markers and successfully validated in a third germplasm set, where they explained up to 19% of phenotypic variation for TKW under moisture stress. These findings confirm the identification of critical loci for drought adaptation derived from wild relatives that can now be readily exploited via molecular breeding. |
| format | Journal Article |
| id | CGSpace137787 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1377872026-01-15T02:16:24Z Genomic regions involved in the control of 1,000-kernel weight in wild relative-derived populations of durum wheat Jabbour, Yaman Shafik Hakim, Mohammad Al-Yossef, Abdallah M. Saleh, Maysoun Al-Dein Shaaban, Ahmad Kabbaj, Hafssa Zaim, Meryem Kleinermann, Charles Bassi, Filippo drought wild relatives grain size nested association mapping nam tkw Terminal drought is one of the most common and devastating climatic stress factors affecting durum wheat (Triticum durum Desf.) production worldwide. The wild relatives of this crop are deemed a vast potential source of useful alleles to adapt to this stress. A nested association mapping (NAM) panel was generated using as a recurrent parent the Moroccan variety ‘Nachit’ derived from Triticum dicoccoides and known for its large grain size. This was recombined to three top-performing lines derived from T. dicoccoides, T. araraticum, and Aegilops speltoides, for a total of 426 inbred progenies. This NAM was evaluated across eight environments (Syria, Lebanon, and Morocco) experiencing different degrees of terminal moisture stress over two crop seasons. Our results showed that drought stress caused on average 41% loss in yield and that 1,000-kernel weight (TKW) was the most important trait for adaptation to it. Genotyping with the 25K TraitGenetics array resulted in a consensus map of 1,678 polymorphic SNPs, spanning 1,723 cM aligned to the reference ‘Svevo’ genome assembly. Kinship distinguished the progenies in three clades matching the parent of origin. A total of 18 stable quantitative trait loci (QTLs) were identified as controlling various traits but independent from flowering time. The most significant genomic regions were named Q.ICD.NAM-04, Q.ICD.NAM-14, and Q.ICD.NAM-16. Allelic investigation in a second germplasm panel confirmed that carrying the positive allele at all three loci produced an average TKW advantage of 25.6% when field-tested under drought conditions. The underlying SNPs were converted to Kompetitive Allele-Specific PCR (KASP) markers and successfully validated in a third germplasm set, where they explained up to 19% of phenotypic variation for TKW under moisture stress. These findings confirm the identification of critical loci for drought adaptation derived from wild relatives that can now be readily exploited via molecular breeding. 2023-11-30 2024-01-16T15:37:58Z 2024-01-16T15:37:58Z Journal Article https://hdl.handle.net/10568/137787 en https://www.genesys-pgr.org/wiews/SYR002 Open Access application/pdf Frontiers Media Yaman Jabbour, Mohammad Shafik Hakim, Abdallah Al-Yossef, Maysoun M. Saleh, Ahmad Al-Dein Shaaban, Hafssa Kabbaj, Meryem Zaim, Charles Kleinermann, Filippo Bassi. (30/11/2023). Genomic regions involved in the control of 1, 000-kernel weight in wild relative-derived populations of durum wheat. Frontiers in Plant Science, 14, pp. 1-15. |
| spellingShingle | drought wild relatives grain size nested association mapping nam tkw Jabbour, Yaman Shafik Hakim, Mohammad Al-Yossef, Abdallah M. Saleh, Maysoun Al-Dein Shaaban, Ahmad Kabbaj, Hafssa Zaim, Meryem Kleinermann, Charles Bassi, Filippo Genomic regions involved in the control of 1,000-kernel weight in wild relative-derived populations of durum wheat |
| title | Genomic regions involved in the control of 1,000-kernel weight in wild relative-derived populations of durum wheat |
| title_full | Genomic regions involved in the control of 1,000-kernel weight in wild relative-derived populations of durum wheat |
| title_fullStr | Genomic regions involved in the control of 1,000-kernel weight in wild relative-derived populations of durum wheat |
| title_full_unstemmed | Genomic regions involved in the control of 1,000-kernel weight in wild relative-derived populations of durum wheat |
| title_short | Genomic regions involved in the control of 1,000-kernel weight in wild relative-derived populations of durum wheat |
| title_sort | genomic regions involved in the control of 1 000 kernel weight in wild relative derived populations of durum wheat |
| topic | drought wild relatives grain size nested association mapping nam tkw |
| url | https://hdl.handle.net/10568/137787 |
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