Targeted knockout of early nodulin-like 3 (MusaENODL3) gene in banana reveals its function in resistance to Xanthomonas wilt disease
Nodulins and nodulin-like proteins play an essential role in the symbiotic associations between legumes and Rhizobium bacteria. Their role extends beyond the leguminous species, as numerous nodulin-like proteins, including early nodulin-like proteins (ENODL), have been identified in various non-legu...
| Main Authors: | , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2024
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/135185 |
| _version_ | 1855534160660135936 |
|---|---|
| author | Ntui, V.O. Tripathi, J. Shah, T. Tripathi, L. |
| author_browse | Ntui, V.O. Shah, T. Tripathi, J. Tripathi, L. |
| author_facet | Ntui, V.O. Tripathi, J. Shah, T. Tripathi, L. |
| author_sort | Ntui, V.O. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Nodulins and nodulin-like proteins play an essential role in the symbiotic associations between legumes and Rhizobium bacteria. Their role extends beyond the leguminous species, as numerous nodulin-like proteins, including early nodulin-like proteins (ENODL), have been identified in various non-leguminous plants, implying their involvement in functions beyond nodulation, such as nutrient transport and growth modulation. Some ENODL proteins have been associated with plant defense against pathogens, as evident in banana infected with Xanthomonas campestris pv. musacearum (Xcm) causing banana Xanthomonas wilt (BXW) disease. Nonetheless, the specific role of ENODL in plant defense remains to be fully elucidated. The MusaENODL3 gene was found to be repressed in BXW-resistant banana progenitor 'Musa balbisiana' and 20-fold upregulated in BXW-susceptible cultivar 'Gonja Manjaya' upon early infection with Xcm. To further unravel the role of the ENODL gene in disease resistance, the CRISPR/Cas9 system was employed to disrupt the MusaENODL3 gene in 'Gonja Manjaya' precisely. Analysis of the enodl3 edited events confirmed the accurate manipulation of the MusaENODL3 gene. Disease resistance and gene expression analysis demonstrated that editing the MusaENODL3 gene resulted in resistance to BXW disease, with 50% of the edited plants remaining asymptomatic. The identification and manipulation of the MusaENODL3 gene highlight its potential as a critical player in plant-pathogen interactions, offering new opportunities for enhancing disease resistance in crops like banana, an important staple food crop and source of income for resource-poor farmers in the tropics. This study provides the first evidence of the direct role of the ENODL3 gene in developing disease-resistant plants. |
| format | Journal Article |
| id | CGSpace135185 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1351852025-12-08T10:11:39Z Targeted knockout of early nodulin-like 3 (MusaENODL3) gene in banana reveals its function in resistance to Xanthomonas wilt disease Ntui, V.O. Tripathi, J. Shah, T. Tripathi, L. bananas musa xanthomonas campestris muscearum Nodulins and nodulin-like proteins play an essential role in the symbiotic associations between legumes and Rhizobium bacteria. Their role extends beyond the leguminous species, as numerous nodulin-like proteins, including early nodulin-like proteins (ENODL), have been identified in various non-leguminous plants, implying their involvement in functions beyond nodulation, such as nutrient transport and growth modulation. Some ENODL proteins have been associated with plant defense against pathogens, as evident in banana infected with Xanthomonas campestris pv. musacearum (Xcm) causing banana Xanthomonas wilt (BXW) disease. Nonetheless, the specific role of ENODL in plant defense remains to be fully elucidated. The MusaENODL3 gene was found to be repressed in BXW-resistant banana progenitor 'Musa balbisiana' and 20-fold upregulated in BXW-susceptible cultivar 'Gonja Manjaya' upon early infection with Xcm. To further unravel the role of the ENODL gene in disease resistance, the CRISPR/Cas9 system was employed to disrupt the MusaENODL3 gene in 'Gonja Manjaya' precisely. Analysis of the enodl3 edited events confirmed the accurate manipulation of the MusaENODL3 gene. Disease resistance and gene expression analysis demonstrated that editing the MusaENODL3 gene resulted in resistance to BXW disease, with 50% of the edited plants remaining asymptomatic. The identification and manipulation of the MusaENODL3 gene highlight its potential as a critical player in plant-pathogen interactions, offering new opportunities for enhancing disease resistance in crops like banana, an important staple food crop and source of income for resource-poor farmers in the tropics. This study provides the first evidence of the direct role of the ENODL3 gene in developing disease-resistant plants. 2024-05 2023-12-11T10:35:59Z 2023-12-11T10:35:59Z Journal Article https://hdl.handle.net/10568/135185 en Open Access application/pdf Wiley Ntui, V.O., Tripathi, J., Shah, T. & Tripathi, L. (2023). Targeted knockout of early nodulin-like 3 (MusaENODL3) gene in banana reveals its function in resistance to Xanthomonas wilt disease. Plant Biotechnology Journal, 1-12. |
| spellingShingle | bananas musa xanthomonas campestris muscearum Ntui, V.O. Tripathi, J. Shah, T. Tripathi, L. Targeted knockout of early nodulin-like 3 (MusaENODL3) gene in banana reveals its function in resistance to Xanthomonas wilt disease |
| title | Targeted knockout of early nodulin-like 3 (MusaENODL3) gene in banana reveals its function in resistance to Xanthomonas wilt disease |
| title_full | Targeted knockout of early nodulin-like 3 (MusaENODL3) gene in banana reveals its function in resistance to Xanthomonas wilt disease |
| title_fullStr | Targeted knockout of early nodulin-like 3 (MusaENODL3) gene in banana reveals its function in resistance to Xanthomonas wilt disease |
| title_full_unstemmed | Targeted knockout of early nodulin-like 3 (MusaENODL3) gene in banana reveals its function in resistance to Xanthomonas wilt disease |
| title_short | Targeted knockout of early nodulin-like 3 (MusaENODL3) gene in banana reveals its function in resistance to Xanthomonas wilt disease |
| title_sort | targeted knockout of early nodulin like 3 musaenodl3 gene in banana reveals its function in resistance to xanthomonas wilt disease |
| topic | bananas musa xanthomonas campestris muscearum |
| url | https://hdl.handle.net/10568/135185 |
| work_keys_str_mv | AT ntuivo targetedknockoutofearlynodulinlike3musaenodl3geneinbananarevealsitsfunctioninresistancetoxanthomonaswiltdisease AT tripathij targetedknockoutofearlynodulinlike3musaenodl3geneinbananarevealsitsfunctioninresistancetoxanthomonaswiltdisease AT shaht targetedknockoutofearlynodulinlike3musaenodl3geneinbananarevealsitsfunctioninresistancetoxanthomonaswiltdisease AT tripathil targetedknockoutofearlynodulinlike3musaenodl3geneinbananarevealsitsfunctioninresistancetoxanthomonaswiltdisease |